Functional genomics using transgenic zebrafish

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We successfully developed novel genetic methods by using the Tol2 transposon system such as gene trapping, enhancer trapping, the Gal4-UAS system and in vivo transposition system. By utilizing these methods, we created more than 600 of transgenic fish lines that express GFP or Gal4 in specific tissues, cells and organs. By analyzing these transgenic fish, we studied the function of important developmental genes. Further, we constructed a database of the transgenic fish resource and facilitated collaboration with researchers all over the world. Thus, our achievements greatly contributed advances of the study of vertebrate genes.