Basic and applied genomics of enterohemorrhagic Escherichia coli and related enteropathogens

通过对肠出血性大肠杆菌(EHEC)和相关肠道病原体进行一系列基因组测序和功能分析。我们成功地建立了O157 EHEC菌株的快速分型系统，获得了三种主要非O157 EHEC的全基因组序列，并加深了我们对这些病原体III型分泌机制和其他毒力系统的功能和进化机制的理解。此外，我们通过比较元基因组学分析，成功地阐明了成人和婴儿肠道微生物区系的遗传和功能差异，并获得了3个肠道细菌物种(4个菌株，包括1个不可培养的细菌)的全基因组序列。

By performing a series of genome sequencing and functional analyses of enterohemorrhagic Ecsherichia coli (EHEC) and related enteropathogens. we succeeded in developing a rapid O157 EHEC strain typing system, obtaining the complete genome sequences of three major non-O157 EHECs, and improving our understanding of functions and evolution mechanisms of type III secretion mechanisms and other virulence systems of these pathogens. In addition, we succeeded in clarifying the genetic and functional differences of adult and infant gut microbiota by comparative metagenomic analysis and obtaining the complete genome sequences of 3 bacterial species (4 strains, including an unculturable bacterium) residing in intestine.