Escherichia coli pan-genome analysis using next-generation DNA sequencing technologies

我们收集了大量不同致病类型和来源的大肠杆菌菌株，并使用下一代测序仪进行了大规模的基因组测序。这些测序菌株与已测序菌株的基因组比较提供了大肠杆菌的全基因组信息，并揭示了肠出血性大肠杆菌或其他致病类型的一些基因组特征。此外，我们还发现在分离的肠出血性大肠杆菌或致病性大肠杆菌(EPEC)中包含了相当数量的阿尔伯蒂埃希氏菌，并获得了具有代表性的阿尔伯蒂埃希氏菌的序列草案。

We collected numerous Escherichia coli strains of various pathotypes and origins and performed a large scale genome sequencing using next-generation sequencers. Genomic comparison of these sequenced strains and already sequenced strains provided the pan-genome information of E. coli and revealed several genomic features characteristic to enterohemorrhagic E. coli (EHEC) or other pathotypes. Furthermore, we found a significant number of E. arbertii strains are included in the strains isolated as EHEC or enteropathogenic E. coli (EPEC) and obtained draft sequences of representative E. arbertii strains.