植物細胞の分裂を制御するMyb転写因子

Myb转录因子控制植物细胞分裂

• 批准号: 17027015
• 负责人: 伊藤 正樹
• 金额: $ 2.69万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17027015/
• 关键词: 植物細胞; 細胞分裂; 細胞増殖; 転写因子; シロイヌナズナ; Myb; 細胞質分裂

シロイヌナズナの3R-Myb遺伝子は、A-type(MYB3R1とMYB3R4)、B-type(MYB3R2)、およびC-type(MYB3R3とMYB3R5)に構造上分類される。本年度はC-type 3R-Mybの機能解析を中心に行った。C-type 3R-Mybの破壊株では一群のG2/M期遺伝子が野生型よりも強く発現することから、転写抑制因子である可能性が示された。C-type 3R-Myb遺伝子の破壊株に見られるG2/M期遺伝子のmRNAレベルの上昇は、発生初期の若い葉よりも、発生の進んだ葉において顕著にみられること、またA-type 3R-MybとC-type 3R-Mybを同時に破壊してもA-type 3R-Myb単独の破壊株に見られるサイトキネシスの異常が抑圧されないことから、C-type 3R-Mybは増殖中の細胞では転写抑制因子としての働きをもたず、その主要な働きは増殖を停止した(あるいは停止しつつある)組織におけるG2/M期遺伝子の転写抑制であると考えられた。さらにC-type 3R-Myb破壊株において見られるG2/M期遺伝子の発現の上昇は、A-type 3R-Mybを同時に破壊することにより強調された。一方、標的遺伝子の一つであるKNOLLEのプロモーター中に存在するMSAエレメントに変異を導入すると、根端などの増殖の盛んな組織ではGUSレポーターの発現が減少するが、逆に発生の進んだ葉などでは顕著にGUSの発現を上昇させた。これらの結果から、A-type 3R-Mybは増殖の盛んな組織ではG2/M期遺伝子の転写活性化因子として働くが、逆に発生の進んだ組織ではC-type 3R-Mybと共同して転写抑制因子として働くのではないかと考えられた。今後この仮説をGtype 3R-Mybの破壊株に見られる表現型と標的遺伝子の発現との関連から検証したいと考えている。

Please classify 3R-Myb (MYB3R1MYB3R4), B-type (MYB3R2), and C-type (MYB3R3MYB3R5). This year's C-type 3R-Myb machine will be able to analyze the central bank account. A group of C-type 3R-Myb isolates were isolated from G2BG M stage, and the wild type was strongly detected. The possibility of writing inhibitors was not detected. During the period of C-type 3R-Myb, there is no significant difference between the two parts of the system. During the period of the first two years of life, there are some problems in the mRNA system. In the early stages of life, if you are in the early stages of your life, and if you are in the early stages of your life, you will not be able to do this at the same time. At the same time, you will not be able to break the A-type 3R-Myb of the whole company. In C-type 3R-Myb colonization, there is no significant difference between the two groups in terms of writing inhibitors and major inhibitors of colonization. Tissue tissue is responsible for the inhibition of G _ 2. In the same time that the C-type 3R-Myb was broken, the G2cyanM phase was overloaded, and at the same time, the A-type 3R-Myb was overloaded. On the one hand, there is a significant increase in the number of KNOLLE cells in the system. In this case, there is a significant increase in the growth rate of the tissue, which is the main cause of the disease. On the other hand, there is a significant difference between the two parts of the body, the first part of the system, the first part of the system, the first part of the system. In the first place, there is a significant difference between the two parts of the KNOLLE system. In this case, there is a significant difference between the two parts of the system, and the results show that there is a significant difference between the GUS and the GUS. The results of this study showed that A-type 3R-Myb colonization was successful in tissue culture, and the activation factor was written in the G2cyr M phase. The activation factor was expressed in the reverse transcriptional tissue, and the expression of the inhibitory factor was detected in the tissue tissue. The inhibition factor was expressed in the C-type 3R-Myb gene. In the future, there will be a lot of damage to the Gtype 3R-Myb plant. The table type will be broken.

项目成果

The alc-GR system : a modifild alc gene switch designed for use in plant tissue culture.

alc-GR 系统：一种修饰的 alc 基因开关，设计用于植物组织培养。

• 发表时间: 2005
• 期刊: Plant Physiol. 138
• 作者: Roberts;G.K.
• 通讯作者: G.K.

Transcriptional activation of tobacco E2F is repressed by co-transfection with the retinoblastoma-related protein: cyclin D expression overcomes this repressor activity

• DOI: 10.1007/s11103-004-6601-x
• 发表时间: 2004
• 期刊: Plant Molecular Biology
• 影响因子: 5.1
• 作者: Kenji Uemukai;H. Iwakawa;S. Kosugi;Sarah de Uemukai;K. Kato;E. Kondorosi;J. Murray;Masaki Ito;A. Shinmyō;M. Sekine

Conservation and diversification of three-repeat Myb transcription factors in plants

• DOI: 10.1007/s10265-005-0192-8
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF PLANT RESEARCH
• 影响因子: 2.8
• 作者: Ito, M

R1R2R3-Myb proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana

• DOI: 10.1242/dev.02801
• 发表时间: 2007-03-15
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Haga, Nozomi;Kato, Kiichi;Ito, Masaki
• 通讯作者: Ito, Masaki

Elicitor-induced down-reguiation of cell cycle-related genes in tobacco cells.

诱导子诱导的烟草细胞中细胞周期相关基因的下调。

• 发表时间: 2006
• 期刊: Plant Cell Environ. 29
• 作者: Suzeki;K.
• 通讯作者: K.