Electron paramagnetic resonance spectrometer for rapid scan, freeze quench and kinetic measurements
用于快速扫描、冷冻淬灭和动力学测量的电子顺磁共振波谱仪
基本信息
- 批准号:500203554
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Major Research Instrumentation
- 财政年份:2022
- 资助国家:德国
- 起止时间:2021-12-31 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
In our interdisciplinary mechanistic-synthetic research that paves the path for the control of redox (electron transfer) processes in living systems, biomimetic catalysis and chemical energy conversion, almost all investigated compounds and their catalytic or stoichiometric reactions are paramagnetic or implicate the formation/decay of paramagnetic species, respectively. Thus, the application of electron paramagnetic resonance (EPR) as a direct and non-invasive technique is an essential tool to characterise compounds of interest (complexes with redox-active metal centres and/or ligands and free radical species such as superoxide, nitric oxide, hydroxyl radical etc.) and clarify the mechanism of their transformation. In particular, it will be crucial for kinetic studies, trapping/characterizing of reactive intermediates and real-time monitoring of fast (catalytic) processes when UV/Vis and NMR methods exhibit their limitations, which is often the case in processes investigated by us that involve multicomponent paramagnetic reaction mixtures (e.g. when reactants, intermediates and products are all paramagnetic) and two or more parallel-running (catalytic) reactions. Thus, the nature or our research requires a rather unconventional EPR device capable of rapid scan (RS), freeze quench (FQ) and stopped-flow (SF) measurements (RS/FQ/SF-EPR). Such a multifunctional, high-performance EPR instrument with unique rapid-mixing and rapid-scan measurement capability does not exist at LMU and to best of our knowledge not even in Germany. For the objectives of our kinetic/mechanistic approaches, the required EPR device, apart from standard EPR measurements (CW, X-band mode), should be able to meet the following requirements: (i) to operate at Q-band microwaves, (ii) to utilise two cooling systems using liquid helium and liquid nitrogen, (iii) to easily handle freeze-quench experiments to catch and characterize short-lived radical intermediates, (iv) to be compatible with the home-built, dielectric ring resonator that is essential to construct the EPR stopped-flow equipment, (v) to operate in rapid-scan mode using the RS-resonator to carry out time-resolved measurements of very short-lived radical/paramagnetic species and (vi) to operate with a mixing (flow) resonator to study short-lived radicals generated in biological medium. Such instrumentation will allow broad application (within our collaborations or service measurements) of the EPR technique for interested groups in different fields and will be indispensable for further scientific achievements of our ongoing and planned projects, contributing to the development of efficient catalysts, redox-modulators, therapeutics or diagnostic tools. It will also allow us to push the boundaries of possible EPR methodology by constructing a rather unique rapid-scan EPR stopped-flow device of improved resolution that would currently be operative in the field of bioinorganic chemistry and chemical energy conversion.
在我们的跨学科机械合成研究中,为生命系统中的氧化还原(电子转移)过程的控制,仿生催化和化学能量转换铺平了道路,几乎所有研究的化合物及其催化或化学计量反应都是顺磁性的,或者分别涉及顺磁性物质的形成/衰变。因此,电子顺磁共振(EPR)作为一种直接和非侵入性技术的应用是鉴定感兴趣的化合物(具有氧化还原活性金属中心和/或配体和自由基物质如超氧化物、一氧化氮、羟基自由基等的络合物)的基本工具。并阐明其转化机制。特别是,它对于动力学研究、活性中间体的捕获/表征和快速反应的实时监测将是至关重要的。当UV/维斯和NMR方法显示出其局限性时,通常在我们研究的涉及多组分顺磁反应混合物的过程中就是这种情况(例如,当反应物、中间体和产物都是顺磁性时)和两个或更多个平行运行的(催化)反应。因此,自然界或我们的研究需要一个相当非常规的EPR设备能够快速扫描(RS),冷冻淬火(FQ)和停流(SF)测量(RS/FQ/SF-EPR)。这种具有独特的快速混合和快速扫描测量能力的多功能、高性能EPR仪器在LMU并不存在,据我们所知,甚至在德国也不存在。对于我们的动力学/机械方法的目标,除了标准EPR测量外,(CW、X波段模式),应能满足以下要求:(i)在Q波段微波下操作,(ii)利用使用液氦和液氮的两个冷却系统,(iii)轻松处理冷冻猝灭实验以捕获和表征短寿命自由基中间体,(iv)与自制的介电环形谐振器兼容,这对于构建EPR停流设备至关重要,(v)使用RS谐振器在快速扫描模式下操作,以进行非常短寿命的自由基/顺磁物质的时间分辨测量,以及(vi)与混合(流动)谐振器一起操作,以研究生物介质中产生的短寿命自由基。这种仪器将允许EPR技术在不同领域的感兴趣群体中的广泛应用(在我们的合作或服务测量范围内),并且对于我们正在进行和计划的项目的进一步科学成就是不可或缺的,有助于开发有效的催化剂,氧化还原调节剂,治疗或诊断工具。它还将使我们能够通过构建一个相当独特的快速扫描EPR停流装置来推动可能的EPR方法的界限,该装置具有更高的分辨率,目前可用于生物无机化学和化学能量转换领域。
项目成果
期刊论文数量(0)
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其他文献
吉治仁志 他: "トランスジェニックマウスによるTIMP-1の線維化促進機序"最新医学. 55. 1781-1787 (2000)
Hitoshi Yoshiji 等:“转基因小鼠中 TIMP-1 的促纤维化机制”现代医学 55. 1781-1787 (2000)。
- DOI:
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LiDAR Implementations for Autonomous Vehicle Applications
- DOI:
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2021 - 期刊:
- 影响因子:0
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吉治仁志 他: "イラスト医学&サイエンスシリーズ血管の分子医学"羊土社(渋谷正史編). 125 (2000)
Hitoshi Yoshiji 等人:“血管医学与科学系列分子医学图解”Yodosha(涉谷正志编辑)125(2000)。
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Effect of manidipine hydrochloride,a calcium antagonist,on isoproterenol-induced left ventricular hypertrophy: "Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,K.,Teragaki,M.,Iwao,H.and Yoshikawa,J." Jpn Circ J. 62(1). 47-52 (1998)
钙拮抗剂盐酸马尼地平对异丙肾上腺素引起的左心室肥厚的影响:“Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,
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