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体内時計ニューロンにおける機能成熟プロセスの解明

阐明生物钟神经元的功能成熟过程

基本信息

批准号：
18021012
负责人：
池田真行
金额：
$ 4.61万
依托单位：
University of Toyama
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

本研究は、体内時計細胞が網膜からの光入力を完成させる前後において、発生学的にどのように機能成熟を果たすかという問題に取り組んできた。これまでの成果をまとめると(1)網膜と視交叉上核(SCN)の共培養モデルを試み、in vitroでの回路形成を試みた。また、視交叉上核のモデルとしてガン化プロジェニター細胞(SCN2.2細胞)の分化誘導実験や、Yellow Cameleonセンサー遺伝子の安定発現株の作成を行なった。(2)グリシン作動性網膜アマクリン細胞においてコレシストキニンA受容体が発現し、この受容体欠損が体内時計への光入力を著しく減弱させることを明らかにした。一方で、網膜からの入力をうけるグルタミン酸作動性のSCNニューロンには、コレシストキニンA受容体が発現していないこともあわせて突き止めた。近年、光受容が可能な網膜神経節細胞から体内時計への光入力経路が注目を集めているが、われわれの実験から、クラシカルフォトレセプター-双極細胞-アマクリン細胞-神経節細胞といった別の光入力経路も重要であることが示唆された(FASEB Jにコレスポンディングオーサーとして発表)。(3)ショウジョウバエを用いて光入力と体内時計細胞が共存する培養モデルの作製に成功した。また、体内時計と光入力経路を単離した条件で培養することにも成功した。Per-luciferase発現系統やYellow Cameleon発現系統を用いての培養にも成功しており、per遺伝子発現リズムや細胞内カルシウム濃度リズムの画像化も可能となっている。この結果、ショウジョウバエの体内時計細胞の振動は、中枢神経系を介した光入力が重要な役割を果たしていることを突き止めた(論文投稿中)。

In this study, in vivo, the cell network monitoring system was used to complete the monitoring system before and after the test, and the physiology test machine was able to improve the performance of the computer system. The main results are as follows: (1) the upper nucleus of the network video chiasma (SCN) is co-cultured to form a test circuit for in vitro transmission. In the nucleus of the visual crossover, the differentiation and differentiation of the cells (SCN2.2 cells) lead to the differentiation of the cells, and the cells of the Yellow Cameleon cells are stable and stable. (2) the activity of the network, the cell, the cell, the recipient, the recipient and the recipient. On the one hand, the network is responsible for the action of the SCN. The recipient is responsible for the failure of the recipient. In recent years, light tolerance may be used in the network sensor system to monitor the performance of the network sensor system. In recent years, the light tolerance device has been used to monitor the performance of the network. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, light tolerance may be used in the network environment. In recent years, the light tolerance device has been used in the network. In recent years, the light tolerance device has been used in the network environment. In recent years, the light tolerance device has been used in the network environment. In recent years, the light tolerance device has been used in the network environment. In recent years, the light tolerance device has been used in the network environment. In recent years, the light tolerance device (3) the co-existence of cell culture was successfully performed by using the light to enter the body. In vivo and in vivo, the light entry force is measured and the conditions are tested to determine the success of the test. The Per-luciferase system Yellow Cameleon shows that the system system is successfully tested with the system, and the per system is used to detect the intracellular temperature in the system. It is possible that the image will be affected. The results of the experiment, the timing of cell vibration in vivo, and the central nervous system were used to introduce the important operation of the system (in the article contribution).