Neuroanatomy/Histology/Brain Injection Core

神经解剖学/组织学/脑注射核心

• 批准号: 10677758
• 负责人: Laurent Gautron
• 金额: $ 22.91万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-20 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10677758
• 关键词: Biological Assay; Brain; Cannulations; Cerebrospinal Fluid; Collection; Energy Metabolism; Ensure; Equipment; Exercise; Frozen Sections; Genes; Histologic; Histology; Human Resources; Immunohistochemistry; In Situ Hybridization; Injections; Light Microscope; Link; Metabolic; Microdissection; Microscopy; Neuroanatomy; Neurons; Operative Surgical Procedures; Paraffin; Periodicals; Population; Protocols documentation; RNA; Reagent; Research; Research Personnel; Resources; Role; Services; Slide; Technical Expertise; Tissues; Training; Validation; cost effective; energy balance; exercise training; experimental study; fluorescence microscope; histological stains; histological studies; laser capture microdissection; member; mouse model; prevent; programs

ABSTRACT The Neuroanatomy/Histology/Brain Injection Core (Core C) will facilitate neuroanatomical and histological studies for PPG Members. It will be used regularly by investigators from all 3 projects described in the PPG. Dr. Laurent Gautron will coordinate these services and resources, and will provide periodic training of personnel on the use of the equipment. Core C will also be an essential component of the characterization and validation of gene specific deletion mouse models proposed by all 3 projects. Core C is equipped with the equipment and expertise to provide the following services: Core C will provide the following services:  Routine histology  Immunolabeling  RNA in situ hybridization  Stereotaxic surgeriesMicroscopy  Brain microdissection  RNA isolation and qPCR analysis. The technical expertise in Cores C will provide multiple benefits to project investigators:  Centralized Core services are more cost effective and prevent the duplication of reagents across Program investigators.  Uniform standards and protocols will ensure that results from all Projects can directly be compared  Provides technical expertise for protocols that are not routinely performed by Program investigators

摘要 神经解剖/组织学/脑注射核心(核心C)将促进神经解剖和组织学 针对PPG成员的研究。它将由PPG中描述的所有3个项目的调查人员定期使用。Dr。 Laurent Gautron将协调这些服务和资源，并将提供定期人员培训 关于设备的使用。核心C也将是表征和验证的重要组成部分 所有3个项目提出的基因特异性缺失小鼠模型。核心C配备了设备和 提供以下服务的专业知识： 核心C将提供以下服务： 常规组织学 免疫标记 核糖核酸原位杂交 立体定向手术显微镜 脑显微解剖 核糖核酸提取及定量聚合酶链式反应分析。 C核心的技术专长将给项目调查员带来多重好处： 集中化核心服务更具成本效益，并防止试剂在 项目调查员。 统一的标准和协议将确保所有项目的结果可以直接进行比较 为不是由计划调查人员常规执行的协议提供技术专业知识