Module 2: Histology and Imaging

模块 2：组织学和成像

• 批准号: 10700856
• 负责人: Xingjun Fan
• 金额: $ 24.61万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10700856
• 关键词: Blindness; Cells; Center Core Grants; DNA Sequence Alteration; Data; Detection; Disease; Doctor of Philosophy; Electron Microscopy; Electrons; Funding; Genetic Diseases; Goals; Histologic; Histology; Image; Image Analysis; Individual; Laboratories; Laser Scanning Confocal Microscopy; Location; Methods; Microscope; Microscopic; Morphology; Patients; Process; Production; Proteins; Publications; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Scientist; Services; Stains; Technical Expertise; Technology; Tissues; Training; Vision; Vision Disorders; Vision research; Visual System; Visualization; experimental study; instrument; instrumentation; laser capture microdissection; member; multi-photon; multiphoton microscopy; protein protein interaction; sample fixation; skills; superresolution microscopy; tissue processing

The histology and imaging module allows investigators to understand visual function and disease at a cellular/subcellular level. It offers outstanding technical expertise, training and state-of-the-art instrumentation needed to conduct rigorous histologic/morphologic studies and image analyses. The expertise and instrumentation available in this module far exceed resources of individual investigators’ laboratories. Histologic services offered by a highly skilled technician include guidance in isolation/orientation/fixation/embedding of ocular tissues (or cells), routine and specialized sectioning and staining, as well as training for immunodetection studies. A PhD level scientist with extensive expertise in the visual system is available for electron microscopic studies permitting ultrastructural analysis of ocular tissue as well as protein-protein interactions at the subcellular level. This module features state-of-the-art imaging instrumentation including access to super resolution microscopy, multiple confocal microscopes (upright, inverted, multiphoton) and laser capture microdissection. A highly skilled PhD level scientist, with excellent background in vision research, oversees these instruments and will assist investigators in generating high quality, reliable, reproducible data. She will assist users with production of publication-quality images. The module will provide technical expertise and instrumentation to achieve the following three aims: Aim 1: Provide core investigators with expertise for isolation and orientation of ocular tissues/cells, appropriate histologic processing to meet specific experimental needs, guidance in immunodetection of proteins, including EM immunodetection methods. Aim 2: Provide expert technical assistance and training in the use of laser scanning confocal microscopy, multiphoton microscopy, super resolution microscopy and laser capture microdissection. Aim 3: Provide expert technical assistance in the use of electron microscopy and image capture. Support of this module will provide resources needed by nearly all of our vision researchers to engage in high impact research and discovery for patients suffering from blindness and visual disorders.

组织学和成像模块使研究者能够在一个特定的时间内了解视觉功能和疾病。 细胞/亚细胞水平。它提供卓越的技术专长、培训和最先进的仪器 需要进行严格的组织学/形态学研究和图像分析。的专门知识和 该单元中可用的仪器远远超过了单个研究人员实验室的资源。组织学 由高度熟练的技术人员提供的服务包括隔离/定向/固定/嵌入的指导， 眼组织（或细胞），常规和专门切片和染色，以及免疫检测培训 问题研究一位在视觉系统方面具有丰富专业知识的博士级科学家可用于电子显微镜检查。 研究允许眼组织的超微结构分析以及亚细胞中蛋白质-蛋白质相互作用， 水平该模块具有最先进的成像仪器，包括超分辨率 显微镜，多个共聚焦显微镜（直立，倒置，多光子）和激光捕获显微切割。一 高技能博士水平的科学家，具有良好的视觉研究背景，监督这些仪器， 将协助研究者生成高质量、可靠、可重复的数据。她将帮助用户 制作出版质量的图像。该单元将提供技术专门知识和仪器， 实现以下三个目标：目标1：为核心研究人员提供隔离和定向的专业知识 眼组织/细胞，满足特定实验需要的适当组织学处理， 蛋白质的免疫检测，包括EM免疫检测方法。目标2：提供专业技术 激光扫描共聚焦显微镜、多光子显微镜、超声波显微镜、 分辨率显微镜和激光捕获显微切割。目标3：提供使用方面的专家技术援助 电子显微镜和图像捕捉的技术。对该模块的支持将为几乎所有 我们的视力研究人员为失明患者进行高影响力的研究和发现， 视觉障碍