UHR-Mass Spectrometry System for Metabolomics and Mass Spectrometry Imaging

用于代谢组学和质谱成像的 UHR 质谱系统

• 批准号: 503287583
• 金额: --
• 依托单位: Universität Bielefeld
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/503287583?language=en
• 关键词: UHR; Mass; Spectrometry; System; Metabolomics

The Center for Biotechnology (CeBiTec) at Bielefeld University operates the “Genomics” technology platform (TP). This TP makes "omics techniques" of genome, transcriptome, proteome and metabolome research available to a wide group of users. In addition to laboratory research, data storage and data analysis are centrally supported by locally developed and commercially available software offers. This is closely linked to the support provided by the German Network for Bioinformatics Infrastructure (de.NBI), which is coordinated in Bielefeld. In addition, extensive training courses on data evaluation are offered. With the establishment of the new "Faculty of Medicine", existing TPs are to be transferred to cross-faculty "core facilities (CFs)" and expanded. This application is part of the planned restructuring and expansion.For many working groups, metabolomic research has been an integral part of the molecular biological processing of various questions for many years. The biotechnological optimization of unicellular production organisms is a focus of CeBiTec, which is essentially dependent on the methods of metabolomic research. Part of this work is the analysis and optimization of metabolic pathways for the production of amino acids, amines, pharmacological molecules, terpenoids and polysaccharides. Another focus is the metabolic physiology of the plant, whereby regulatory mechanisms of secondary metabolite production and stress adaptation are analyzed. Finally, animal and human samples and cell cultures are analyzed in order to better understand physiological (mal) functions in the context of diseases. With the establishment of the new Faculty of Medicine, this area will become increasingly important.This technology was established as part of the DFG initiative “Imaging Mass Spectrometry (MSI)”. MSI, also in combination with multimodal imaging, is gaining increasing interest among many users of the TP. Newly appointed colleagues from the medical faculty show particular interest in MSI.Several GC-MS systems are available for metabolomic analyzes. For LC-MS analyzes, however, there is only one very old and, due to its age, not very sensitive device. In the case of isoform metabolites, there is no possibility of differentiation.The device applied for here is intended to serve both UHPLC-based metabolomic analysis and MSI analysis. In an increasing number of projects, the separation of isobaric and isomeric molecules and a very sensitive, imaging, detection of metabolites is important. High mass accuracy and MS / MS capability are required for identification. The device applied for is to be used across the university as a central device for LC-MS analysis of metabolites in the core facility "Omics", which is currently being set up.

比勒费尔德大学的生物技术中心（CeBiTec）运营着“基因组学”技术平台（TP）。该TP使基因组、转录组、蛋白质组和代谢组研究的“组学技术”可供广大用户使用。除了实验室研究外，数据存储和数据分析还得到当地开发和商业软件的集中支持。这与德国生物信息基础设施网络提供的支持密切相关，该网络在比勒费尔德进行协调。此外，还提供关于数据评价的广泛培训课程。随着新的“医学院”的成立，现有的TP将被转移到跨学院的“核心设施（CF）”和扩大。这项应用是计划中的重组和扩展的一部分。对于许多工作组来说，代谢组学研究多年来一直是各种问题的分子生物学处理的一个组成部分。单细胞生产生物的生物技术优化是CeBiTec的重点，这基本上取决于代谢组学研究的方法。这项工作的一部分是分析和优化生产氨基酸，胺，药理学分子，萜类化合物和多糖的代谢途径。另一个重点是植物的代谢生理学，分析次生代谢产物产生和胁迫适应的调节机制。最后，分析动物和人类样本和细胞培养物，以更好地了解疾病背景下的生理（异常）功能。随着新医学院的成立，这一领域将变得越来越重要。这项技术是DFG倡议“成像质谱（MSI）"的一部分。MSI也与多模态成像相结合，在TP的许多用户中获得了越来越多的兴趣。新上任的医学院同事对MSI表现出特别的兴趣。几种GC-MS系统可用于代谢组学分析。然而，对于LC-MS分析，只有一个非常旧的设备，并且由于其年龄，不是非常敏感。对于异构体代谢物，不存在区分的可能性，此处申请的设备预期用于基于超高效液相色谱的代谢组学分析和MSI分析。在越来越多的项目中，同量异位素和异构体分子的分离以及非常灵敏的代谢物的成像检测是重要的。鉴别需要高质量准确度和MS / MS能力。申请的设备将在整个大学中用作目前正在建立的核心设施“组学”中代谢物LC-MS分析的中心设备。