UHR-Mass Spectrometry System for Metabolomics and Mass Spectrometry Imaging

Bielefeld大学的生物技术中心（CeBiTec）运营着“基因组学”技术平台（TP）。该TP使基因组、转录组、蛋白质组和代谢组研究的“组学技术”可供广泛的用户使用。除了实验室研究外，数据存储和数据分析由本地开发和商业软件提供集中支持。这与在比勒费尔德协调的德国生物信息学基础设施网络（de.NBI）提供的支持密切相关。此外，还提供了关于数据评价的广泛培训班。随着新“医学院”的成立，现有的教学计划将被转移到跨学院的“核心设施”，并进行扩展。该应用程序是计划中的重组和扩展的一部分。对于许多工作组来说，代谢组学研究多年来一直是分子生物学处理各种问题的一个组成部分。单细胞生产生物的生物技术优化是CeBiTec的一个重点，它本质上依赖于代谢组学研究的方法。这项工作的一部分是分析和优化代谢途径的生产氨基酸，胺，药理分子，萜类化合物和多糖。另一个重点是植物的代谢生理，由此分析了次生代谢物的产生和胁迫适应的调节机制。最后，分析动物和人类样本和细胞培养物，以便更好地了解疾病背景下的生理（不良）功能。随着新医学院的成立，这一领域将变得越来越重要。这项技术是作为DFG倡议“成像质谱（MSI）”的一部分而建立的。MSI，也与多模态成像相结合，在TP的许多用户中引起越来越多的兴趣。医学院新任命的同事对MSI表现出特别的兴趣。几种GC-MS系统可用于代谢组学分析。然而，对于LC-MS分析，只有一个非常古老的，由于它的年龄，不是很敏感的设备。在同型代谢物的情况下，没有分化的可能性。本装置用于uhplc代谢组学分析和MSI分析。在越来越多的项目中，等压和同分异构体分子的分离和非常灵敏的成像、代谢物的检测是很重要的。鉴定需要高质量精度和MS / MS能力。申请的设备将作为核心设施“组学”中代谢物LC-MS分析的中心设备在整个大学中使用，该设施目前正在建立中。

The Center for Biotechnology (CeBiTec) at Bielefeld University operates the “Genomics” technology platform (TP). This TP makes "omics techniques" of genome, transcriptome, proteome and metabolome research available to a wide group of users. In addition to laboratory research, data storage and data analysis are centrally supported by locally developed and commercially available software offers. This is closely linked to the support provided by the German Network for Bioinformatics Infrastructure (de.NBI), which is coordinated in Bielefeld. In addition, extensive training courses on data evaluation are offered. With the establishment of the new "Faculty of Medicine", existing TPs are to be transferred to cross-faculty "core facilities (CFs)" and expanded. This application is part of the planned restructuring and expansion.For many working groups, metabolomic research has been an integral part of the molecular biological processing of various questions for many years. The biotechnological optimization of unicellular production organisms is a focus of CeBiTec, which is essentially dependent on the methods of metabolomic research. Part of this work is the analysis and optimization of metabolic pathways for the production of amino acids, amines, pharmacological molecules, terpenoids and polysaccharides. Another focus is the metabolic physiology of the plant, whereby regulatory mechanisms of secondary metabolite production and stress adaptation are analyzed. Finally, animal and human samples and cell cultures are analyzed in order to better understand physiological (mal) functions in the context of diseases. With the establishment of the new Faculty of Medicine, this area will become increasingly important.This technology was established as part of the DFG initiative “Imaging Mass Spectrometry (MSI)”. MSI, also in combination with multimodal imaging, is gaining increasing interest among many users of the TP. Newly appointed colleagues from the medical faculty show particular interest in MSI.Several GC-MS systems are available for metabolomic analyzes. For LC-MS analyzes, however, there is only one very old and, due to its age, not very sensitive device. In the case of isoform metabolites, there is no possibility of differentiation.The device applied for here is intended to serve both UHPLC-based metabolomic analysis and MSI analysis. In an increasing number of projects, the separation of isobaric and isomeric molecules and a very sensitive, imaging, detection of metabolites is important. High mass accuracy and MS / MS capability are required for identification. The device applied for is to be used across the university as a central device for LC-MS analysis of metabolites in the core facility "Omics", which is currently being set up.