Triple Quadrupole/Linear ion TRAP Mass Spectrometer with HPLC System

带 HPLC 系统的三重四极杆/线性离子 TRAP 质谱仪

• 批准号: 7595521
• 负责人: Joseph DiDonato
• 金额: $ 50万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595521
• 关键词: Affect; Amino Acid Sequence; Biological Markers; Clinic; Clinical; Clinical and Translational Science Awards; Commit; Custom; Development; Disease; Distant; Early Diagnosis; Evaluation; Foundations; Funding; Grant; Health; High Pressure Liquid Chromatography; Human; Human Resources; Hybrids; Institution; Ions; Laboratories; Maintenance; Mass Spectrum Analysis; Ohio; Operative Surgical Procedures; Outcome; Peptide Sequence Determination; Productivity; Program Research Project Grants; Research; Research Personnel; Research Project Grants; Resources; Sampling; Schedule; Sensitivity and Specificity; Services; System; Technology; Time; Travel; Universities; analytical tool; base; instrument; instrumentation; mass spectrometer; member; prognostic; programs; public health relevance; translational study; treatment program

DESCRIPTION (provided by applicant): The purpose of this application is to seek funds for the purchase of a hybrid triple quadrupole/linear ion trap (ABI 4000 QTRAP) mass spectrometry system with multiplex HPLC interface. This instrument will expand and upgrade mass spectrometry capabilities within the Northeastern Ohio region, advancing research programs within the Cleveland Clinic Foundation (CCF) and its partner institution, Case Western Reserve University (CWRU), other Cleveland Clinical and Translational Science Award (CTSA) member institutions and Cleveland State University (CSU). Major Users all have NIH-funded research grants, including recent multiple Program Project Grants and SCCOR programs, all of which have projects that are currently conducted using mass spectrometry to ask, investigate and answer key questions concerning major diseases that affect human health. Due to the full utilization of available instrumentation in the region, many investigators now have to schedule time many months in advance to obtain access to this technology and/or travel to distant (>60 miles) institutions. As detailed in the application, supported projects primarily include biomarker discovery and clinical/translational studies nearly all of which also utilize the NCRR-funded Cleveland CTSA. The requested mass spectrometer provides the high through-put capabilities that will assure timely access to this type of instrumentation, while not sacrificing versatility, sensitivity and specificity required for these projects. This analytical activity will be conducted within our existing Mass Spectrometry Core II, which provides support to investigators at CCF, CWRU, CSU and neighboring institutions by performing structural elucidation studies, biomarker quantification, protein sequencing/identification, and related services. Mass spectrometry is increasingly being used as an analytical tool by CCF and partner institution investigators, with demand greatly outpacing availability, resulting in significant delays in access, and hampered productivity. The acquisition of the requested mass spectrometer is essential to alleviate the backlog of samples waiting to be analyzed. CCF has committed substantial resources to the development of mass spectrometry in the region, including custom renovation of laboratory space for Mass Spectrometry Core II, personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term. PUBLIC HEALTH RELEVANCE: The scientific investigators, who comprise the major user group for this shared mass spectrometry instrumentation application, all have NIH-funded research programs including being part of multiple, very large clinical sample-based grants. These investigators have projects that are currently conducted, in part, using the available but overburdened mass spectroscopy instrumentation to ask, investigate and answer key questions concerning major diseases that affect human health. These projects, which would benefit greatly from use of the requested instrumentation, primarily include disease biomarker discovery and clinical/translational studies that will facilitate early detection of disease states and/or provide prognostic evaluation of the disease state or treatment program based upon clinical sample outcomes.

描述（由申请人提供）：本申请的目的是寻求资金购买具有多重 HPLC 接口的混合三重四极杆/线性离子阱 (ABI 4000 QTRAP) 质谱系统。该仪器将扩展和升级俄亥俄州东北部地区的质谱能力，推进克利夫兰诊所基金会 (CCF) 及其合作机构凯斯西储大学 (CWRU)、其他克利夫兰临床和转化科学奖 (CTSA) 成员机构和克利夫兰州立大学 (CSU) 内的研究项目。主要用户均拥有 NIH 资助的研究补助金，包括最近的多项计划项目补助金和 SCCOR 计划，所有这些项目目前都使用质谱法来提出、调查和回答有关影响人类健康的重大疾病的关键问题。由于该地区现有仪器的充分利用，许多研究人员现在必须提前几个月安排时间，以获得这项技术和/或前往遥远（> 60 英里）的机构。正如申请中详细说明的，支持的项目主要包括生物标志物发现和临床/转化研究，几乎所有这些项目也利用 NCRR 资助的克利夫兰 CTSA。所需的质谱仪提供高通量能力，确保及时访问此类仪器，同时不牺牲这些项目所需的多功能性、灵敏度和特异性。这项分析活动将在我们现有的质谱核心 II 内进行，该核心通过进行结构阐明研究、生物标志物定量、蛋白质测序/鉴定和相关服务，为 CCF、CWRU、CSU 和邻近机构的研究人员提供支持。质谱法越来越多地被 CCF 和合作机构研究人员用作分析工具，但需求远远超过了可用性，导致获取严重延迟，并影响了生产力。获取所需的质谱仪对于缓解等待分析的样品积压至关重要。 CCF 已投入大量资源用于该地区质谱技术的发展，包括质谱核心 II 实验室空间的定制改造、监督其运营的人员以及确保其长期运营和维护的财政计划。公共健康相关性：构成该共享质谱仪器应用程序主要用户组的科学研究人员都拥有 NIH 资助的研究项目，包括参与多个基于临床样本的大型资助项目。这些研究人员目前正在进行的项目部分使用现有但负担过重的质谱仪器来询问、调查和回答有关影响人类健康的重大疾病的关键问题。这些项目将大大受益于所需仪器的使用，主要包括疾病生物标志物发现和临床/转化研究，这些研究将有助于疾病状态的早期检测和/或根据临床样本结果提供疾病状态或治疗方案的预后评估。