Triple Quadrupole/Linear ion TRAP Mass Spectrometer with HPLC System

带 HPLC 系统的三重四极杆/线性离子 TRAP 质谱仪

• 批准号: 7595521
• 负责人: Joseph DiDonato
• 金额: $ 50万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595521
• 关键词: Affect; Amino Acid Sequence; Biological Markers; Clinic; Clinical; Clinical and Translational Science Awards; Commit; Custom; Development; Disease; Distant; Early Diagnosis; Evaluation; Foundations; Funding; Grant; Health; High Pressure Liquid Chromatography; Human; Human Resources; Hybrids; Institution; Ions; Laboratories; Maintenance; Mass Spectrum Analysis; Ohio; Operative Surgical Procedures; Outcome; Peptide Sequence Determination; Productivity; Program Research Project Grants; Research; Research Personnel; Research Project Grants; Resources; Sampling; Schedule; Sensitivity and Specificity; Services; System; Technology; Time; Travel; Universities; analytical tool; base; instrument; instrumentation; mass spectrometer; member; prognostic; programs; public health relevance; translational study; treatment program

DESCRIPTION (provided by applicant): The purpose of this application is to seek funds for the purchase of a hybrid triple quadrupole/linear ion trap (ABI 4000 QTRAP) mass spectrometry system with multiplex HPLC interface. This instrument will expand and upgrade mass spectrometry capabilities within the Northeastern Ohio region, advancing research programs within the Cleveland Clinic Foundation (CCF) and its partner institution, Case Western Reserve University (CWRU), other Cleveland Clinical and Translational Science Award (CTSA) member institutions and Cleveland State University (CSU). Major Users all have NIH-funded research grants, including recent multiple Program Project Grants and SCCOR programs, all of which have projects that are currently conducted using mass spectrometry to ask, investigate and answer key questions concerning major diseases that affect human health. Due to the full utilization of available instrumentation in the region, many investigators now have to schedule time many months in advance to obtain access to this technology and/or travel to distant (>60 miles) institutions. As detailed in the application, supported projects primarily include biomarker discovery and clinical/translational studies nearly all of which also utilize the NCRR-funded Cleveland CTSA. The requested mass spectrometer provides the high through-put capabilities that will assure timely access to this type of instrumentation, while not sacrificing versatility, sensitivity and specificity required for these projects. This analytical activity will be conducted within our existing Mass Spectrometry Core II, which provides support to investigators at CCF, CWRU, CSU and neighboring institutions by performing structural elucidation studies, biomarker quantification, protein sequencing/identification, and related services. Mass spectrometry is increasingly being used as an analytical tool by CCF and partner institution investigators, with demand greatly outpacing availability, resulting in significant delays in access, and hampered productivity. The acquisition of the requested mass spectrometer is essential to alleviate the backlog of samples waiting to be analyzed. CCF has committed substantial resources to the development of mass spectrometry in the region, including custom renovation of laboratory space for Mass Spectrometry Core II, personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term. PUBLIC HEALTH RELEVANCE: The scientific investigators, who comprise the major user group for this shared mass spectrometry instrumentation application, all have NIH-funded research programs including being part of multiple, very large clinical sample-based grants. These investigators have projects that are currently conducted, in part, using the available but overburdened mass spectroscopy instrumentation to ask, investigate and answer key questions concerning major diseases that affect human health. These projects, which would benefit greatly from use of the requested instrumentation, primarily include disease biomarker discovery and clinical/translational studies that will facilitate early detection of disease states and/or provide prognostic evaluation of the disease state or treatment program based upon clinical sample outcomes.

描述（由申请人提供）：本申请的目的是为购买具有多路复用HPLC接口的质谱系统寻求资金以购买混合三倍四倍/线性离子陷阱（ABI 4000 QTRAP）质谱系统。该工具将扩大和升级俄亥俄州东北部地区的质谱能力，推进克利夫兰诊所基金会（CCF）及其合作伙伴机构Case Western Reserve University（CWRU），克利夫兰其他克利夫兰临床和转化科学奖（CTSA）成员机构和克利夫兰州立大学（CSU）的研究计划。主要用户都拥有NIH资助的研究补助金，其中包括最近的多个计划项目赠款和SCCOR计划，所有这些都有目前使用质谱法进行的项目，以询问，调查和回答有关影响人类健康的主要疾病的关键问题。由于该地区可用的仪器充分利用，现在许多调查人员必须提前安排时间数月以获取该技术和/或前往遥远（> 60英里）机构的访问。如应用程序中所述，受支持的项目主要包括生物标志物发现和临床/翻译研究，几乎所有这些研究都使用了NCRR资助的Cleveland CTSA。请求的质谱仪提供了很高的贯穿能力，可以确保及时访问此类仪器，同时不牺牲这些项目所需的多功能性，敏感性和特异性。这种分析活性将在我们现有的质谱核心II中进行，该核心核心II通过进行结构性阐明研究，生物标志物量化，蛋白质测序/识别和相关服务来为CCF，CWRU，CSU和邻近机构的研究人员提供支持。 CCF和合作伙伴机构研究人员越来越多地将质谱法用作分析工具，需求大大超过了可用性，从而导致访问的延迟和妨碍生产率。获得所需的质谱仪的获取对于减轻等待分析的样品积压至关重要。 CCF已为该地区的质谱开发提供了大量资源，包括对质谱核心II的实验室空间进行定制翻新，监督其运营的人员以及一项财政计划，以确保其长期运营和维护。公共卫生相关性：该共享质谱仪器应用的主要用户组的科学研究人员都有NIH资助的研究计划，包括成为多个非常大的基于临床样本的赠款的一部分。这些调查人员目前使用可用但负担重大的质谱工具来询问，调查和回答有关影响人类健康的主要疾病的关键问题的部分项目。这些项目将通过使用要求的仪器受益匪浅，主要包括疾病生物标志物发现和临床/转化研究，这些研究将促进疾病状态的早期发现和/或基于临床样本结果对疾病状态或治疗计划的预后评估。