300kV Cryo-TEM

300kV 冷冻透射电镜

• 批准号: 506518771
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/506518771?language=en
• 关键词: 300kV; Cryo; TEM

This proposal aims to set-up a high-end cryo-electron microscopy (cryo-EM) facility that is equipped with instrumentation for the major current techniques of cryo-EM, namely single-particle analysis, cryo-electron tomography (cryo-ET) and electron diffraction as a university core facility. This will allow the University of Freiburg to be competitive in these seminal tech-niques of current structural and cellular biology. To realize this, a high-end 300 kV transmis-sion electron microscope with a fast-counting detector and an energy filter is necessary. For sample preparation in cryo-ET and further analysis, a cryo-focussed ion beam - scanning electron microscope in combination with a correlative light-and electron microscopy set-up is also required. Finally, as these instruments produce exceptionally large amounts of data, a dedicated server and storage arry for preprocessing and data analysis are essential.

该提案旨在建立一个高端冷冻电子显微镜（cryo-EM）设施，配备用于冷冻电子显微镜当前主要技术的仪器，即单粒子分析，冷冻电子断层扫描（cryo-ET）和电子衍射作为大学核心设施。这将使弗赖堡大学在当前结构和细胞生物学的这些开创性技术方面具有竞争力。为了实现这一点，高端300 kV透射电子显微镜与快速计数探测器和能量过滤器是必要的。对于冷冻ET中的样品制备和进一步分析，还需要冷冻聚焦离子束扫描电子显微镜与相关的光学和电子显微镜设置相结合。最后，由于这些仪器会产生非常大量的数据，因此用于预处理和数据分析的专用服务器和存储阵列至关重要。