Glacios 200 kV cryogenic transmission electron microscope (cryo-TEM)

Glacios 200 kV 低温透射电子显微镜 (cryo-TEM)

• 批准号: 10176876
• 负责人: THOMAS B THOMPSON
• 金额: $ 200万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176876
• 关键词: Address; Architecture; Area; Biological; Biology; Cardiovascular system; Collaborations; Communicable Diseases; Complex; Cryoelectron Microscopy; Crystallization; Electron Microscope; Electron Microscopy; Environment; Funding; Institution; Investments; Kentucky; Learning; Life; Malignant Neoplasms; Medical center; Microscope; Modernization; Molecular; Negative Staining; Neurosciences; Ohio; Pediatric Hospitals; Play; Postdoctoral Fellow; Protein Analysis; Research; Research Personnel; Resolution; Sampling; Structural Biologist; Structure; Techniques; Technology; Training; United States National Institutes of Health; Universities; X-Ray Crystallography; cryogenics; detector; graduate student; insight; instrument; instrumentation; macromolecule; programs; structural biology; transmission process

PROJECT SUMMARY Structural biology has played a key role in defining the molecular architecture of life, leading to fundamental insights into the function and biology of macromolecules. Within our Ohio Valley consortium, consisting of the University of Cincinnati, Cincinnati's Children's Hospital Medical Center, Miami University, and University of Kentucky, all have well-established programs in structural biology, with a major focus on X-ray crystallography. This has provided an outstanding training environment for graduate students and postdoctoral fellows to learn modern structural biology approaches to address fundamental questions around biomedically important macromolecules. However, in recent years, cryogenic electron microscopy (cryo-EM) has emerged as an indispensable technique for structural analysis of proteins and molecular complexes due to the fact that it can produce atom-resolution structures without drawbacks inherent to crystallization. Thus, our consortium structural biologists have been actively working to incorporate this technology into their research programs, primarily through collaboration and attempts to access instruments at other institutions. As a result of these efforts, we now have a critical mass of investigators that strongly justifies bringing this technology to our region. The University of Cincinnati has committed to laying the groundwork for this initiative by purchasing a Talos L120C instrument for negative stain analysis and a Vitrobot to prepare grids with a vitrified sample. These investments will allow users the ability to ascertain the quality of biological samples for further cryo-EM analysis. To take us to the next level, we are requesting NIH funding for a Glacios 200 kV microscope with Falcon 4 detector and autoloader, which will be housed in a newly developing cryo-EM facility at the University of Cincinnati. The Glacios will allow us to further optimize samples for structural analysis in house, or provide the justification for use of advanced cryo-EM instrumentation available at other institutions or NIH consortia for higher resolution studies. Collectively, the acquisition of the Glacios will directly benefit over 30 NIH funded investigators working in the focus areas of Cardiovascular, Cancer, Infectious Disease, and Neuroscience.

项目摘要 结构生物学在定义生命的分子体系结构方面发挥了关键作用，导致了基本 对大分子的功能和生物学的见解。在我们的俄亥俄河谷财团内，由 辛辛那提大学，辛辛那提的儿童医院医学中心，迈阿密大学和大学 肯塔基州的结构生物学方面都有良好的计划，重点是X射线晶体学。 这为研究生和博士后研究员提供了出色的培训环境 现代结构生物学方法，以解决有关生物医学重要的基本问题 大分子。然而，近年来，低温电子显微镜（Cryo-EM）已成为 不可或缺的技术用于蛋白质和分子复合物的结构分析，因为它可以 产生原子分辨率结构，而没有结晶固有的缺点。因此，我们的财团 结构生物学家一直在积极努力将这项技术纳入其研究计划， 主要是通过协作和尝试在其他机构访问工具的尝试。结果 努力，我们现在拥有大量的调查人员，这些调查人员有力地证明将这项技术带入我们的地区。 辛辛那提大学致力于通过购买TALOS来为该计划奠定基础 L120C仪器进行阴性染色分析和用玻璃化样品制备网格的玻璃体。这些 投资将使用户能够确定进一步的Cryo-EM的生物样品质量 分析。要将我们带到一个新的水平，我们要求使用NIH资助200 kV显微镜 Falcon 4探测器和自动加载器，该探测器和自动加载器将安置在大学的新开发的低温EM设施中 辛辛那提。 Glacios将使我们能够进一步优化样本以进行内部结构分析，或提供 使用其他机构或NIH财团的使用高级冷冻仪器的理由 高分辨率研究。总体而言，收购冰川将直接受益于30多家NIH资助 在心血管，癌症，传染病和神经科学的重点领域工作的研究人员。