STAR family function and interaction with RBM20 for intervening alternative splicing (A07)

STAR 家族功能以及与 RBM20 的相互作用以干预选择性剪接 (A07)

• 批准号: 508084636
• 金额: --
• 依托单位: Klinik für Kardiologie, Angiologie und Pulmologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/508084636?language=en
• 关键词: STAR; family; function; interaction; RBM20

In this project, we aim to in detail characterise the novel cardiac splice factor SLM2. This includes the fine mapping of the splice targets in the human heart, the deciphering of co-factors and regulator elements and the in-vitro and in-vivo functional characterisation e.g. in conditional knock-out mice. Since we propose an partially antagonistic role of SLM2 and RBM20, we will study combined transgenic iPSC and mouse models. Together, the obtained results will provide mechanistic insights into the novel cardiac splice regulator SLM2 and proof-of-concept of therapeutic manipulations.

在这个项目中，我们的目标是详细描述新的心脏剪接因子SLM2。这包括人类心脏中剪接靶标的精细定位、辅助因子和调节元件的破译以及体外和体内功能表征，例如在条件性敲除小鼠中。由于我们提出了SLM2和RBM20的部分拮抗作用，我们将研究组合的转基因iPSC和小鼠模型。总之，所获得的结果将提供新的心脏剪接调节剂SLM2和治疗操作的概念验证机制的见解。