Cloning of avirulence genes in Pyricularia spp. using super SAGE and comparative analysis of their instability

梨孢霉属无毒基因的克隆。

• 批准号: 20248005
• 负责人: TOSA Yukio
• 金额: $ 29.79万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20248005/
• 关键词: いもち病; 非病原力遺伝子; Magnaporthe oryzae; Pyricularia oryzae; avirulence gene; Manarthe orzae; Pricularia orzae; Avirulence gene; Rice; Blast; blast

We found that AVR-Pita, AVR-Pia, AVR-Pii, AVR-Pik, avirulence genes of the rice blast fungus, are highly variable in its genome location, occurring in chromosomes 1 through 7 and supernumerary chromosomes. They were flanked by retrotransposons, which presumably contributed to their "multiple translocation" across the genome during the course of evolution in response to corresponding resistance genes. We also found an avirulence gene that has not undergone multiple translocation. We suggest that the stability of avirulence genes is predictable on the basis of the their chromosomal locations

我们发现稻瘟病菌的无毒基因AVR-PITA、AVR-Pia、AVR-PII、AVR-Pik的基因组位置具有很高的变异性，发生在1~7号染色体和多生染色体上。它们的侧翼是反转录转座子，据推测，在进化过程中，这些反转录转座子可能导致了它们在基因组中对相应的抗性基因做出反应的“多次易位”。我们还发现了一个没有经历多次易位的无毒基因。我们认为，根据无毒基因的染色体位置可以预测无毒基因的稳定性。

项目成果

Genetic analyses of host species specificity of Mangaporthe oryzae subgroups

稻瘟病菌亚群宿主物种特异性的遗传分析

• 发表时间: 2010
• 作者: Tosa;Y. and Chuma I.
• 通讯作者: Y. and Chuma I.

PWT1, an Avirulence Gene of Magnaporthe oryzae Tightly Linked to the rDNA Locus, Is Recognized by Two Staple Crops, Common Wheat and Barley

• DOI: 10.1094/phyto-100-5-0436
• 发表时间: 2010-05-01
• 期刊: PHYTOPATHOLOGY
• 影响因子: 3.2
• 作者: Chuma, Izumi;Zhan, Su-Wen;Tosa, Yukio
• 通讯作者: Tosa, Yukio

PWT1, an avirulence gene of Magnaporthe oryzae tightly linked to the rDNA locus, is recognized by two staple crops

PWT1 是稻瘟病菌的一个无毒基因，与 rDNA 位点紧密相连，可被两种主要作物识别

• 发表时间: 2010
• 期刊: common wheat and barley. Phytopathology
• 作者: Chuma;I.;Zhan;S. W.;Asano;S.;Nga;N. T. T.;Vy;T. T. P.;Shirai;M.;Ibaragi;K.;and Tosa;Y.
• 通讯作者: Y.

Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species.

• DOI: 10.1371/journal.ppat.1002147
• 发表时间: 2011-07
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Chuma I;Isobe C;Hotta Y;Ibaragi K;Futamata N;Kusaba M;Yoshida K;Terauchi R;Fujita Y;Nakayashiki H;Valent B;Tosa Y
• 通讯作者: Tosa Y

Roles of avirulence genes of Pyricularia species in defining host and nonhost specificity

梨虫无毒基因在宿主和非宿主特异性中的作用

• 发表时间: 2011
• 作者: Tosa;Y.
• 通讯作者: Y.