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Diversification and transposition of avirulence genes in Magnaporthe oryzae prompted by the instability of subtelomeres

亚端粒不稳定性导致的稻瘟病菌无毒基因的多样化和转位

基本信息

批准号：
18380034
负责人：
TOSA Yukio
金额：
$ 11.04万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380034/
关键词：
Mapnaporthe oryzae Pyricularia orvzae Avirulence gene

项目摘要

AVR-Pita is an avirulence gene corresponding-to Pita, a rice gene for resistance to the rice blast fungus, Magnaporthe oryzae. Homologues of this gene are present not only in Oryza isolates, but also in Setaria isolates, Panicum isolates, Digitazia isolates, etc. We cloned these homologues with their flanking regions. A homologue from a Cenchrus isolate was not flanked by any known transposable elements. On the other hand, those from Digitaria isolates were located between two copies of a retrotransposon, Inago 1. Those from Panicum miliaceum and Setaria viridis isolates were flanked by a copy of Inago 1. Those from Oryza sativa and Setana italica isolates were accompanied by several copies of solo LTR of retrotransposons. When a dendrogram was constructed from the DNA sequences of these homologues, the homologue from the Cenchrus isolate was located at the root of the others, while those from Oryza isolates were located at a distal position.To examine the function of these homologues, they were introduced into P2-b, an Oryza Slate virulent on rice cultivar Yashiromochi with Pita. Infection assays with these transformants revealed that the homologues from Digitazia, Panicurn and Setaria Slates maintained their function as avirulence genes. We suggest that this is because these homologues have not had a chance to interact with Pita.

AVR-Pita 是与 Pita 相对应的无毒基因，Pita 是一种抗稻瘟病菌 Magnaporthe oryzae 的水稻基因。该基因的同源物不仅存在于稻分离株中，还存在于狗尾草分离株、黍分离株、马唐分离株等中。我们克隆了这些同源物及其侧翼区域。来自 Cenchrus 分离物的同源物侧翼没有任何已知的转座元件。另一方面，来自马唐属分离株的那些位于反转录转座子 Inago 1 的两个拷贝之间。来自黍和狗尾草分离株的那些分离株的两侧是一个 Inago 1 的拷贝。来自稻和 Setana italica 分离株的那些则伴随有几个反转录转座子的单独 LTR 拷贝。当从这些同源物的 DNA 序列构建树状图时，来自 Cenchrus 分离物的同源物位于其他分离物的根部，而来自 Oryza 分离物的同源物位于远端位置。为了检查这些同源物的功能，将它们引入 P2-b（一种对水稻品种 Yashiromochi with Pita 具有毒力的 Oryza Slate）。对这些转化体的感染测定表明，来自马唐属、黍属和狗尾草的同源物保持了它们作为无毒基因的功能。我们认为这是因为这些同系物没有机会与皮塔相互作用。