Diversification and transposition of avirulence genes in Magnaporthe oryzae prompted by the instability of subtelomeres
亚端粒不稳定性导致的稻瘟病菌无毒基因的多样化和转位
基本信息
- 批准号:18380034
- 负责人:
- 金额:$ 11.04万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
AVR-Pita is an avirulence gene corresponding-to Pita, a rice gene for resistance to the rice blast fungus, Magnaporthe oryzae. Homologues of this gene are present not only in Oryza isolates, but also in Setaria isolates, Panicum isolates, Digitazia isolates, etc. We cloned these homologues with their flanking regions. A homologue from a Cenchrus isolate was not flanked by any known transposable elements. On the other hand, those from Digitaria isolates were located between two copies of a retrotransposon, Inago 1. Those from Panicum miliaceum and Setaria viridis isolates were flanked by a copy of Inago 1. Those from Oryza sativa and Setana italica isolates were accompanied by several copies of solo LTR of retrotransposons. When a dendrogram was constructed from the DNA sequences of these homologues, the homologue from the Cenchrus isolate was located at the root of the others, while those from Oryza isolates were located at a distal position.To examine the function of these homologues, they were introduced into P2-b, an Oryza Slate virulent on rice cultivar Yashiromochi with Pita. Infection assays with these transformants revealed that the homologues from Digitazia, Panicurn and Setaria Slates maintained their function as avirulence genes. We suggest that this is because these homologues have not had a chance to interact with Pita.
AVR-PITA是一个与水稻抗稻瘟病基因PITA相对应的无毒基因。该基因的同源物不仅存在于水稻分离物中,还存在于狗尾草分离物、Panicum分离物、马唐分离物等中。来自一株虎尾草分离物的同源基因侧翼没有任何已知的转座元件。来自马唐菌株的基因位于反转录转座子Inago 1的两个拷贝之间,来自Panicum miliaceum和Setaria viridis菌株的基因位于Inago 1的两侧,来自水稻和狗尾草的基因伴随有多个单独的反转录转座子的LTR拷贝。根据这些同源物的DNA序列构建的树状图显示,来自Cichrus分离物的同源物位于其他同源物的根部,而来自Oryza分离物的同源物位于远端,为了检测这些同源物的功能,将它们导入水稻品种Yashiromochi和Pita的致病菌株P2-b中。对这些转化子的侵染分析表明,来自马唐、Panicurn和狗尾草的同源物保持了它们作为无毒基因的功能。我们认为这是因为这些同系物还没有机会与PITA互动。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
いもち病菌の非病原力遺伝子AVR-Pitaホモログのタイピング、マッピングおよび機能の比較解析
稻瘟病菌无毒基因AVR-Pita同源物的分型、作图及比较功能分析
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:西村一馬;佐々英徳;中馬いづみ
- 通讯作者:中馬いづみ
Dynamics of an avirulence gene locus, AVR-Pita, in Pyricularia spp
Pyrularia spp 无毒基因座 AVR-Pita 的动态
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Izumi;Chuma;Chihiro;Isobe;Hiotshi;Nakayashiki;Shigeyuki;Mayama;Yukio;Tosa
- 通讯作者:Tosa
イネ科植物いもち病菌(Pyricularia spp.)における非病原力遺伝子AVR-Pitaの座乗染色体変動機構
梨锈菌无毒基因AVR-Pita位点介导的染色体变异机制。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Matsubara;K.;K. Shimamura;H. Kodama;H. Watanabe;H. Kokubun;T. Ando;中馬いづみ
- 通讯作者:中馬いづみ
いもち病菌非病原力遺伝子AVR-Pitaの座乗染色体変動過程とその機構
稻瘟菌无毒基因AVR-Pita位点染色体变异过程及其机制
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Izumi;Chuma;Chihiro;Isobe;Hiotshi;Nakayashiki;Shigeyuki;Mayama;Yukio;Tosa;中馬いづみ
- 通讯作者:中馬いづみ
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TOSA Yukio其他文献
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{{ truncateString('TOSA Yukio', 18)}}的其他基金
Mechanisms of evolution of hypervirulent isolates of the wheat blast fungus and identification of resistance genes against it
麦瘟菌高毒力菌株的进化机制及其抗性基因鉴定
- 批准号:
17H01462 - 财政年份:2017
- 资助金额:
$ 11.04万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Mechanisms of host jump and evolution of Pyricularia oryzae Triticum pathotype
稻瘟病菌致病型的宿主跳跃与进化机制
- 批准号:
26292025 - 财政年份:2014
- 资助金额:
$ 11.04万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Why do avirulence genes of the rice blast fungus undergo multiple translocation?-A hypothesis that crabgrass plays a role as their repository
为什么稻瘟病菌的无毒基因会发生多次易位?-马唐充当其储存库的假设
- 批准号:
22658014 - 财政年份:2010
- 资助金额:
$ 11.04万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Cloning of avirulence genes in Pyricularia spp. using super SAGE and comparative analysis of their instability
梨孢霉属无毒基因的克隆。
- 批准号:
20248005 - 财政年份:2008
- 资助金额:
$ 11.04万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Identification of an avirulence gene island in Pyriculazia chromosomes and analyses of its transposition and disorganization
Pyriculazia染色体无毒基因岛的鉴定及其转座和解体分析
- 批准号:
16380036 - 财政年份:2004
- 资助金额:
$ 11.04万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular analysis of species-specific parasitism in Magnaporthe grisea
稻瘟病菌物种特异性寄生的分子分析
- 批准号:
10460022 - 财政年份:1998
- 资助金额:
$ 11.04万 - 项目类别:
Grant-in-Aid for Scientific Research (B).














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