Diversification and transposition of avirulence genes in Magnaporthe oryzae prompted by the instability of subtelomeres

亚端粒不稳定性导致的稻瘟病菌无毒基因的多样化和转位

• 批准号: 18380034
• 负责人: TOSA Yukio
• 金额: $ 11.04万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380034/
• 关键词: Mapnaporthe oryzae; Pyricularia orvzae; Avirulence gene

AVR-Pita is an avirulence gene corresponding-to Pita, a rice gene for resistance to the rice blast fungus, Magnaporthe oryzae. Homologues of this gene are present not only in Oryza isolates, but also in Setaria isolates, Panicum isolates, Digitazia isolates, etc. We cloned these homologues with their flanking regions. A homologue from a Cenchrus isolate was not flanked by any known transposable elements. On the other hand, those from Digitaria isolates were located between two copies of a retrotransposon, Inago 1. Those from Panicum miliaceum and Setaria viridis isolates were flanked by a copy of Inago 1. Those from Oryza sativa and Setana italica isolates were accompanied by several copies of solo LTR of retrotransposons. When a dendrogram was constructed from the DNA sequences of these homologues, the homologue from the Cenchrus isolate was located at the root of the others, while those from Oryza isolates were located at a distal position.To examine the function of these homologues, they were introduced into P2-b, an Oryza Slate virulent on rice cultivar Yashiromochi with Pita. Infection assays with these transformants revealed that the homologues from Digitazia, Panicurn and Setaria Slates maintained their function as avirulence genes. We suggest that this is because these homologues have not had a chance to interact with Pita.

AVR-Pita是一个无毒基因，对应于Pita，一个抗稻瘟病菌的水稻基因。该基因的同源序列不仅存在于稻属分离物中，也存在于狗尾草属分离物、黍属分离物、马唐属分离物等中。一个同源物从蒺藜隔离两侧没有任何已知的转座因子。另一方面，来自马唐分离物的那些位于反转录转座子Inago 1的两个拷贝之间。来自黍和狗尾草分离物的那些侧翼是稻瘟1的拷贝。来自水稻和谷子分离物的那些伴随有几个拷贝的反转录转座子的单独LTR。将这些同源基因导入对水稻品种八代持具Pita毒性的水稻Slate菌株P2-B中，分析其功能。对这些转化体的感染试验表明，来自洋地黄、Panicurn和狗尾草的同源物保持了其无毒基因的功能。我们认为这是因为这些同源物没有机会与皮塔相互作用。

项目成果

いもち病菌の非病原力遺伝子AVR-Pitaホモログのタイピング、マッピングおよび機能の比較解析

稻瘟病菌无毒基因AVR-Pita同源物的分型、作图及比较功能分析

• 发表时间: 2007
• 作者: 西村一馬;佐々英徳;中馬いづみ
• 通讯作者: 中馬いづみ

Dynamics of an avirulence gene locus, AVR-Pita, in Pyricularia spp

Pyrularia spp 无毒基因座 AVR-Pita 的动态

• 发表时间: 2006
• 作者: Izumi;Chuma;Chihiro;Isobe;Hiotshi;Nakayashiki;Shigeyuki;Mayama;Yukio;Tosa
• 通讯作者: Tosa

イネ科植物いもち病菌(Pyricularia spp.)における非病原力遺伝子AVR-Pitaの座乗染色体変動機構

梨锈菌无毒基因AVR-Pita位点介导的染色体变异机制。

• 发表时间: 2006
• 作者: Matsubara;K.;K. Shimamura;H. Kodama;H. Watanabe;H. Kokubun;T. Ando;中馬いづみ
• 通讯作者: 中馬いづみ

いもち病菌非病原力遺伝子AVR-Pitaの座乗染色体変動過程とその機構

稻瘟菌无毒基因AVR-Pita位点染色体变异过程及其机制

• 发表时间: 2006
• 作者: Izumi;Chuma;Chihiro;Isobe;Hiotshi;Nakayashiki;Shigeyuki;Mayama;Yukio;Tosa;中馬いづみ
• 通讯作者: 中馬いづみ