Diversification and transposition of avirulence genes in Magnaporthe oryzae prompted by the instability of subtelomeres

亚端粒不稳定性导致的稻瘟病菌无毒基因的多样化和转位

基本信息

  • 批准号:
    18380034
  • 负责人:
  • 金额:
    $ 11.04万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

AVR-Pita is an avirulence gene corresponding-to Pita, a rice gene for resistance to the rice blast fungus, Magnaporthe oryzae. Homologues of this gene are present not only in Oryza isolates, but also in Setaria isolates, Panicum isolates, Digitazia isolates, etc. We cloned these homologues with their flanking regions. A homologue from a Cenchrus isolate was not flanked by any known transposable elements. On the other hand, those from Digitaria isolates were located between two copies of a retrotransposon, Inago 1. Those from Panicum miliaceum and Setaria viridis isolates were flanked by a copy of Inago 1. Those from Oryza sativa and Setana italica isolates were accompanied by several copies of solo LTR of retrotransposons. When a dendrogram was constructed from the DNA sequences of these homologues, the homologue from the Cenchrus isolate was located at the root of the others, while those from Oryza isolates were located at a distal position.To examine the function of these homologues, they were introduced into P2-b, an Oryza Slate virulent on rice cultivar Yashiromochi with Pita. Infection assays with these transformants revealed that the homologues from Digitazia, Panicurn and Setaria Slates maintained their function as avirulence genes. We suggest that this is because these homologues have not had a chance to interact with Pita.
AVR-Pita 是与 Pita 相对应的无毒基因,Pita 是一种抗稻瘟病菌 Magnaporthe oryzae 的水稻基因。该基因的同源物不仅存在于稻分离株中,还存在于狗尾草分离株、黍分离株、马唐分离株等中。我们克隆了这些同源物及其侧翼区域。来自 Cenchrus 分离物的同源物侧翼没有任何已知的转座元件。另一方面,来自马唐属分离株的那些位于反转录转座子 Inago 1 的两个拷贝之间。来自黍和狗尾草分离株的那些分离株的两侧是一个 Inago 1 的拷贝。来自稻和 Setana italica 分离株的那些则伴随有几个反转录转座子的单独 LTR 拷贝。当从这些同源物的 DNA 序列构建树状图时,来自 Cenchrus 分离物的同源物位于其他分离物的根部,而来自 Oryza 分离物的同源物位于远端位置。为了检查这些同源物的功能,将它们引入 P2-b(一种对水稻品种 Yashiromochi with Pita 具有毒力的 Oryza Slate)。对这些转化体的感染测定表明,来自马唐属、黍属和狗尾草的同源物保持了它们作为无毒基因的功能。我们认为这是因为这些同系物没有机会与皮塔相互作用。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
いもち病菌の非病原力遺伝子AVR-Pitaホモログのタイピング、マッピングおよび機能の比較解析
稻瘟病菌无毒基因AVR-Pita同源物的分型、作图及比较功能分析
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    西村一馬;佐々英徳;中馬いづみ
  • 通讯作者:
    中馬いづみ
Dynamics of an avirulence gene locus, AVR-Pita, in Pyricularia spp
Pyrularia spp 无毒基因座 AVR-Pita 的动态
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Izumi;Chuma;Chihiro;Isobe;Hiotshi;Nakayashiki;Shigeyuki;Mayama;Yukio;Tosa
  • 通讯作者:
    Tosa
イネ科植物いもち病菌(Pyricularia spp.)における非病原力遺伝子AVR-Pitaの座乗染色体変動機構
梨锈菌无毒基因AVR-Pita位点介导的染色体变异机制。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Matsubara;K.;K. Shimamura;H. Kodama;H. Watanabe;H. Kokubun;T. Ando;中馬いづみ
  • 通讯作者:
    中馬いづみ
いもち病菌非病原力遺伝子AVR-Pitaの座乗染色体変動過程とその機構
稻瘟菌无毒基因AVR-Pita位点染色体变异过程及其机制
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Izumi;Chuma;Chihiro;Isobe;Hiotshi;Nakayashiki;Shigeyuki;Mayama;Yukio;Tosa;中馬いづみ
  • 通讯作者:
    中馬いづみ
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TOSA Yukio其他文献

TOSA Yukio的其他文献

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{{ truncateString('TOSA Yukio', 18)}}的其他基金

Mechanisms of evolution of hypervirulent isolates of the wheat blast fungus and identification of resistance genes against it
麦瘟菌高毒力菌株的进化机制及其抗性基因鉴定
  • 批准号:
    17H01462
  • 财政年份:
    2017
  • 资助金额:
    $ 11.04万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Mechanisms of host jump and evolution of Pyricularia oryzae Triticum pathotype
稻瘟病菌致病型的宿主跳跃与进化机制
  • 批准号:
    26292025
  • 财政年份:
    2014
  • 资助金额:
    $ 11.04万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Why do avirulence genes of the rice blast fungus undergo multiple translocation?-A hypothesis that crabgrass plays a role as their repository
为什么稻瘟病菌的无毒基因会发生多次易位?-马唐充当其储存库的假设
  • 批准号:
    22658014
  • 财政年份:
    2010
  • 资助金额:
    $ 11.04万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Cloning of avirulence genes in Pyricularia spp. using super SAGE and comparative analysis of their instability
梨孢霉属无毒基因的克隆。
  • 批准号:
    20248005
  • 财政年份:
    2008
  • 资助金额:
    $ 11.04万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Identification of an avirulence gene island in Pyriculazia chromosomes and analyses of its transposition and disorganization
Pyriculazia染色体无毒基因岛的鉴定及其转座和解体分析
  • 批准号:
    16380036
  • 财政年份:
    2004
  • 资助金额:
    $ 11.04万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular analysis of species-specific parasitism in Magnaporthe grisea
稻瘟病菌物种特异性寄生的分子分析
  • 批准号:
    10460022
  • 财政年份:
    1998
  • 资助金额:
    $ 11.04万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
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