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Diversification and transposition of avirulence genes in Magnaporthe oryzae prompted by the instability of subtelomeres

亚端粒不稳定性导致的稻瘟病菌无毒基因的多样化和转位

基本信息

批准号：
18380034
负责人：
TOSA Yukio
金额：
$ 11.04万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380034/
关键词：
Mapnaporthe oryzae Pyricularia orvzae Avirulence gene

项目摘要

AVR-Pita is an avirulence gene corresponding-to Pita, a rice gene for resistance to the rice blast fungus, Magnaporthe oryzae. Homologues of this gene are present not only in Oryza isolates, but also in Setaria isolates, Panicum isolates, Digitazia isolates, etc. We cloned these homologues with their flanking regions. A homologue from a Cenchrus isolate was not flanked by any known transposable elements. On the other hand, those from Digitaria isolates were located between two copies of a retrotransposon, Inago 1. Those from Panicum miliaceum and Setaria viridis isolates were flanked by a copy of Inago 1. Those from Oryza sativa and Setana italica isolates were accompanied by several copies of solo LTR of retrotransposons. When a dendrogram was constructed from the DNA sequences of these homologues, the homologue from the Cenchrus isolate was located at the root of the others, while those from Oryza isolates were located at a distal position.To examine the function of these homologues, they were introduced into P2-b, an Oryza Slate virulent on rice cultivar Yashiromochi with Pita. Infection assays with these transformants revealed that the homologues from Digitazia, Panicurn and Setaria Slates maintained their function as avirulence genes. We suggest that this is because these homologues have not had a chance to interact with Pita.

AVR-PITA是一个与水稻抗稻瘟病基因PITA相对应的无毒基因。该基因的同源物不仅存在于水稻分离物中，还存在于狗尾草分离物、Panicum分离物、马唐分离物等中。来自一株虎尾草分离物的同源基因侧翼没有任何已知的转座元件。来自马唐菌株的基因位于反转录转座子Inago 1的两个拷贝之间，来自Panicum miliaceum和Setaria viridis菌株的基因位于Inago 1的两侧，来自水稻和狗尾草的基因伴随有多个单独的反转录转座子的LTR拷贝。根据这些同源物的DNA序列构建的树状图显示，来自Cichrus分离物的同源物位于其他同源物的根部，而来自Oryza分离物的同源物位于远端，为了检测这些同源物的功能，将它们导入水稻品种Yashiromochi和Pita的致病菌株P2-b中。对这些转化子的侵染分析表明，来自马唐、Panicurn和狗尾草的同源物保持了它们作为无毒基因的功能。我们认为这是因为这些同系物还没有机会与PITA互动。