Identification of an avirulence gene island in Pyriculazia chromosomes and analyses of its transposition and disorganization

Pyriculazia染色体无毒基因岛的鉴定及其转座和解体分析

• 批准号: 16380036
• 负责人: TOSA Yukio
• 金额: $ 9.66万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380036/
• 关键词: Magnaporthe; Pyricularia; avirulence gene

The significance of AVR1-CO39, an avirulence gene of the blast fungus corresponding to Pi-CO39(t) in rice cultivars, during the evolution and differentiation of the blast fungus was evaluated by studying its function and distribution in Pyricularia spp. When the presence/absence of AVR1-CO39 was plotted on a dendrogram constructed from rDNA sequences, a perfect parallelism was observed between its distribution and the phylogeny of Pyricularia isolates. AVR1-CO39 homologs were exclusively present in one species, P.oryzae, suggesting that AVR1-CO39 appeared during the early stage of evolution of P.oryzae. Transformation assays showed that all the cloned homologs tested are functional as an avirulence gene, indicating that selection has maintained their function. Nevertheless, Oryza isolates (isolates virulent on Oryza spp.) in P. oryzae were exceptionally non-carriers of AVR1-CO39. All Oryza isolates suffered from one of the two types of known rearrangements at the Avr1-CO39 locus, i.e., G type and J type. These types were congruous to the two major lineages of Oryza isolates from Japan determined by MGR586 and MAGGY. These results indicate that AVR1-CO39 was lost during the early stage of evolution of the Oryza-specific subgroup of P.oryzae. Interestingly, its corresponding resistance gene, Pi-CO39(t) is not widely distributed in Oryza spp.Homologs of AVR-Pita, an avirulence gene corresponding to Pita, were widely distributed not only in P.oryzae, but also in P.grisea and P.spp. Interestingly, the chromosomes with the homologs were different from isolates to isolates. Analyses of flanking regions of these homologs suggested that the insertion of transposable elements into their flanking regions gave them mobility.

短句来源通过研究稻瘟病菌中Pi-CO39(t)对应的无毒基因AVR1-CO39在稻瘟病菌进化和分化过程中的功能和分布，评价了AVR1-CO39在稻瘟病菌进化和分化过程中的重要意义。AVR1-CO39同源物仅存在于一个物种p.r oryzae中，表明AVR1-CO39出现在p.r oryzae进化的早期阶段。转化实验表明，所有克隆的同源物都具有无毒基因的功能，表明选择保持了它们的功能。然而，稻瘟病菌中的稻瘟病菌（对稻瘟病菌有毒力的稻瘟病菌）异常地不携带AVR1-CO39。所有的Oryza分离株在Avr1-CO39位点上都存在两种已知重排类型中的一种，即G型和J型。这些类型与日本稻系MGR586和MAGGY鉴定的两个主要系一致。这些结果表明，AVR1-CO39在P.oryzae的oryza特异性亚群的进化早期丢失。有趣的是，其对应的抗性基因Pi-CO39(t)在稻瘟病属中分布并不广泛，而与Pita对应的无毒基因AVR-Pita的同源基因不仅在稻瘟病属中广泛分布，而且在稻瘟病属和稻瘟病属中也广泛分布。有趣的是，具有同源物的染色体在不同的分离株之间是不同的。对这些同源物侧翼区域的分析表明，将转座因子插入到它们的侧翼区域使它们具有流动性。

项目成果

Evolution of an avirulence gene AVR1-CO39 concomitant with the evolution and differentiation of Magnaporthe oryzae

无毒基因AVR1-CO39的进化伴随着稻瘟病菌的进化和分化

• 发表时间: 2005
• 期刊: Molecular Plant-Microbe Interactions 18(11)
• 作者: Hondo;D.;Hase;S.;Kanayama;Y.;Yoshikawa;N.;Takenaka;S.;Takahashi;H.;Yukio TOSA
• 通讯作者: Yukio TOSA

Evolution of an avirulence gene AVR1-C039 concomitant with the evolution and differentiation of Magnaporthe oryzae

伴随着稻瘟病菌进化和分化的无毒基因AVR1-C039的进化

• 发表时间: 2005
• 期刊: Molecular Plant-Microbe Interactions 18(11)
• 作者: Hondo;D.;et al.;Yukio TOSA
• 通讯作者: Yukio TOSA