Development of the basic technology for tooth regenerative therapy system

牙齿再生治疗系统基础技术开发

• 批准号: 20249078
• 负责人: TSUJI Takashi
• 金额: $ 31.12万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20249078/
• 关键词: 再生; 歯; 再生医療; 幹細胞; 歯胚

In current research on whole-tooth regenerative therapy, a basic strategy is being pursued in which a bioengineered tooth germ is induced to develop into a fully functional tooth. We developed a three-dimensional organ-germ culture method for the reconstitution a bioengineered organ germ. We successfully demonstrated that our bioengineered tooth germ could develop a fully functioning tooth, which has hardness for masticatory potential, the functional responsibility against a mechanical stress, and perceptive potentials of neural fibers. Furthermore, we demonstrated a further development in this regard in which a bioengineered tooth unit comprising mature tooth, periodontal ligament and alveolar bone, was successfully transplanted into a properly-sized bony hole in the alveolar bone through bone integration by recipient bone remodeling in a murine transplantation model system. These results showed that the bioengineered tooth germ and mature tooth unit could regenerate a fully functioning tooth and an organ replacement regenerative therapy might be feasible.

在当前关于全齿再生疗法的研究中，正在采用一种基本策略，其中诱导生物工程牙齿发育为功能齐全的牙齿。我们开发了一种三维器官培养方法，用于重建生物工程的器官种植。我们成功地证明了我们的生物工程牙齿可能会产生功能齐全的牙齿，该牙齿具有咀嚼电位的硬度，针对机械应力的功能责任以及神经纤维的感知潜力。此外，在这方面，我们证明了进一步的发展，在这种方面中，在鼠类移植模型系统中，受体重塑，通过受体重塑，通过受体骨重塑，成功地将牙齿，牙周韧带和肺泡骨包含成熟牙齿，牙周韧带和肺泡骨的生物工程装置。这些结果表明，生物工程的牙齿细菌和成熟的牙齿单位可能会再生功能齐全的牙齿，并且可能可行地替换器官的再生疗法。

项目成果

Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells In Vitro and In Vivo

• DOI: 10.1016/j.joen.2008.11.024
• 发表时间: 2009-03-01
• 期刊: JOURNAL OF ENDODONTICS
• 影响因子: 4.2
• 作者: Okamoto, Yosuke;Sonoyama, Wataru;Kuboki, Takuo
• 通讯作者: Kuboki, Takuo

The regulation of tooth morphogenesis is associated with epithelial cell proliferation and the expression of Sonic hedgehog through epithelial-mesenchymal interactions

• DOI: 10.1016/j.bbrc.2011.01.052
• 发表时间: 2011-02-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ishida, Kentaro;Murofushi, Mayumi;Tsuji, Takashi
• 通讯作者: Tsuji, Takashi

組織再生による口腔機能の再生へ向けて

通过组织再生实现口腔功能的再生

• 发表时间: 2010
• 作者: 谷口誠;有本隆文;五十嵐武;窪木拓男;園山亘
• 通讯作者: 園山亘

Tooth Regenera Therapy as a Future Organ Replacement Regenerative Therapy

牙齿再生疗法作为未来的器官替代再生疗法

• 发表时间: 2010
• 作者: Akiyama K;Ohga N;Hida Y;Kawamoto T;Sadamoto Y;Ishikawa S;Akino T;Kondoh M;Maishi N;Matsuda A;Inoue N;Shindoh M;Hida K;齋藤謙悟;山本則子,片倉直子,他;辻孝
• 通讯作者: 辻孝

再生歯による移植システムの開発(II)-再生歯ユニットによる歯槽骨と歯の生理機能の回復-

使用再生牙齿的移植系统的开发（II） -使用再生牙齿单元恢复牙槽骨和牙齿生理功能-

• 发表时间: 2011
• 作者: 大島正充;水野光政;今村彩;森田梨津子;小川美帆;山崎大道;山本照子;齋藤正寛;辻孝
• 通讯作者: 辻孝