Molecular cloning and characterization of genes responsible for the diapause-initiation and the diapause-termination induced by cold in Bombyx mori eggs

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We cloned pnd-1 and-2 genes that have been known as genes initiating Bombyx mori embryonic diapause, using both procedures of positional cloning and RNA interference. We also cloned a strong candidate gene connected the diapause hormone signal to the expression of pnd genes, using DNA microarray analysis. We isolated a gene for heat shock transcription factor(HSF) as a factor transducing the 5℃signal. HSF was shown to bind to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities.