Molecular epidemiological study on lifestyles, inflammation and diabetes mellitus in a population-based cohort

在目前的研究项目中，我们在九州大学队列研究中进行了一项为期5年的后续调查，一项关于与生活有关的疾病的前瞻性研究，以及基于基线与血清高敏感性CRP和全球红蛋白（HbA 1c）关系的绩效交叉部门研究。福冈市东病房的12， 948名居民年龄为49-76人。关于他们， 12， 628人同意进行基因打字分析。总共有12， 694个被邀请参加对生活方式因素和发病率的邮件调查。居住在福冈市东区的居民也被要求捐献血液进行实验室测量。在2010年2月至2012年3月期间， 11， 606（91%）对邮件调查作出了回应，10， 427（82%）参与了血液采样。跨部门研究产生了以下的发现。吸烟和身体质量指数（BMI）是男性血清CRP最重要的决定因素，而BMI是女性的一个因素。一个健康的膳食模式特征是由蔬菜和水果的高强度与较低CRP水平相关的，但发现了一个相当小的贡献量。咖啡饮料与男性中较低的CRP水平有关，但与女性无关。作为表示HbA 1c，酒精使用显示了男女双方的反向关联，而没有与咖啡使用的关联。血清白萝卜素是强有力的，与血清CRP和HbA 1c每个相关。基因型分析以1， 500名男子和1， 500名妇女的随机样本进行了分析。如果我们选择了12种遗传性多态性，那么我们认为它们可能在与炎症的关系中发挥功能性。CRP基因（rs3091244和rs 1205）和APOE多态（E3> E4> E2）中的两种多态性与CRP水平密切相关。

In the present research project, we carried out a 5-year follow-up survey in the Kyushu University Cohort Study, a prospective study on lifestyle-related diseases, and performed cross-sectional studies based on the baseline survey in relation to serum high-sensitivity CRP and glycated hemoglobin(HbA1c). The cohort consists of 12, 948 residents aged 49-76 in the East Ward of Fukuoka City. Of them, 12, 628 persons gave consent to genotyping analysis. A total of 12, 694 subjects were invited to participate in the mail survey on lifestyle factors and morbidity. Residents who lived in the East Ward of Fukuoka City were also asked to donate venous blood for laboratory measurements. During the period from February 2010 to March 2012, 11, 606(91%) responded to the mail survey, and 10, 427(82%) participated in the blood sampling. Cross-sectional studies produced the following findings. Smoking and body mass index(BMI) were the most important determinants of serum CRP in men, and BMI was such a factor in women. A healthy dietary pattern characterized by high intakes of vegetables and fruits was associated with lower CRP levels, but the magnitude of the contribution was found to be very small. Coffee drinking was associated with lower CRP levels in men, but not in women. As regards HbA1c, alcohol use showed an inverse association in both men and women while there was no association with coffee use. Serum bilirubin was strongly, inversely associated with serum CRP and HbA1c each. Genotyping analysis was carried out in a random sample of 1, 500 men and 1, 500 women. We selected 12 genetic polymorphisms, which were considered to be potentially functional in relation to inflammation. Two polymorphisms in the CRP gene(rs3091244およびrs1205) and the APOE polymorphism(E3> E4> E2) were strongly associated with CRP levels.