aptamer

通过固定在钻石上的短DNA和RNA适配子来检测特定的靶标。1)利用基于荧光染料的光子法和基于场效应晶体管(FET)的电位法两种方法对HIV-Tat蛋白(靶标)和血小板衍生生长因子(PDGF)的适体进行检测。通过两种不同的检测机制，讨论了核酸适体与靶分子的结合。2)三磷酸腺苷(ATP)在其适配子上有标记，但在ATP上无标记，检测到清晰。3)放置在微流道底部的FET阵列中的不同晶体管对PDGF进行了选择性检测。它引出了集成微生物系统的基础技术。

Specific targets were detected by short DNA and RNA aptamers immobilized on diamond. 1) HIV-TAT protein(target) and platelet derived growth factor (PDGF) were detected by their aptamers using two methods such as photonic method based on fluorescence dye and potentiometric method based on field effect transistor (FET). The bonding between aptamer and their targets were discussed by the two different detecting mechanisms. 2) Adenosine-triphosphate (ATP) has been clearly detected by without-marker on ATP, but with-marker on its aptamer. It shows the possibility of label free aptamer sensor, 3) PDGF were selectively detected by different transistors in FET arrays which are placed at the bottom of micro flow channels. It leads to the basic technology for integrated micro bio-systems.