aptamer

通过固定在金刚石上的短DNA和RNA适体来检测特异性靶标。1)分别采用基于荧光染料的光子法和基于场效应晶体管（FET）的电位法两种方法，利用核酸适体检测HIV-TAT蛋白（靶标）和血小板衍生生长因子（PDGF）。通过两种不同的检测机制讨论了适体与靶分子的结合情况。2)三磷酸腺苷（ATP）是一种无标记物，但有标记物的核酸适体。3）在微流通道的底部放置不同的FET阵列，通过不同的晶体管选择性地检测PDGF。它导致了集成微生物系统的基本技术。

Specific targets were detected by short DNA and RNA aptamers immobilized on diamond. 1) HIV-TAT protein(target) and platelet derived growth factor (PDGF) were detected by their aptamers using two methods such as photonic method based on fluorescence dye and potentiometric method based on field effect transistor (FET). The bonding between aptamer and their targets were discussed by the two different detecting mechanisms. 2) Adenosine-triphosphate (ATP) has been clearly detected by without-marker on ATP, but with-marker on its aptamer. It shows the possibility of label free aptamer sensor, 3) PDGF were selectively detected by different transistors in FET arrays which are placed at the bottom of micro flow channels. It leads to the basic technology for integrated micro bio-systems.