Two Photon Laser Scanning Microscope for chronic in-vivo experiments

此处要求的用于活动小鼠慢性体内成像的双光子激光扫描显微镜将使Anna-Sophia Wahl博士-作为她在LMU慕尼黑神经解剖学副教授的任命的一部分-继续她的研究，她已经与她在苏黎世大学的小组开始了这项研究。显微镜应特别用于揭示脑损伤后神经元修复机制的基本原理。该小组在复杂的行为评估过程中使用小鼠的双光子钙成像作为一种核心方法，以揭示同一动物健康和病理条件下编码特定感觉运动和记忆功能的单个神经元的命运。我们的目标是了解和表征存活的神经元是如何被招募参与修复过程的。此外，通过使用光学操作对存活的神经元进行不同的刺激，将增强塑料重新布线以恢复脑损伤后丧失或受损的功能。与深度学习算法和化学遗传学相结合的相同实验装置也将与精神病学系的分子和行为神经生物学单元合作应用于其他神经和精神疾病模型。这里提出的仪器在其配置上是独特的，并且将是在LMU慕尼黑校园Innenstadt安装用于慢性体内成像的最先进的成像资源的关键仪器，该成像资源目前在那里不可用，并且对于加强校园Innenstadt的基础神经科学小组，特别是解剖学和精神病学系至关重要。此外，该显微镜应用于LMU慕尼黑卓越卡斯特“SyNergy”内的学生和博士后的合作和培训。

The here requested 2photon laser scanning microscope for chronic in-vivo imaging in active mice shall enable Dr. Anna-Sophia Wahl – as part of her appointment as associate professor for neuroanatomy at LMU Munich – to continue her research, which she has started with her group at the University of Zurich. The microscope shall in particularly be used to uncover fundamental principles of neuronal repair mechanisms after brain injury. The group uses 2photon calcium imaging in mice during sophisticated behavioral assessments as a central method to unveil the fate of individual neurons encoding specific sensorimotor and memory functions in the healthy and pathological condition within the same animal. The goal is to understand and characterize how surviving neurons are recruited to participate in repair processes. Furthermore, by distinct stimulation of surviving neurons using optical manipulation plastic rewiring shall be enhanced to restore lost or impaired function after brain injury. The same experimental set-up in combination with deep learning algorithms and chemogenetics will be also applied for other neurological and psychiatric disease models in collaboration with the Molecular and Behavioral Neurobiology Unit of the Department of Psychiatry. The here proposed instrument is unique in its configuration and will be a key instrument to install state-of the art imaging resources for chronic in-vivo imaging at the Campus Innenstadt of LMU Munich, which is currently not available there and will be essential to strengthen basic neuroscience groups at Campus Innenstadt, in particular at the Department of Anatomy and Psychiatry. Furthermore, the microscope shall be used for collaborations and training of students and postdocs within the Excellence Custer “SyNergy” at LMU Munich.