Raman Confocal Spectrometer

共焦拉曼光谱仪应用于这里将在生物光谱电化学组，这是最近成立的物理和理论化学研究所在波恩大学的实验研究中发挥核心作用。计划的研究工作分为两个总体方向：I）氧化还原生物催化金属蛋白的操作性研究，催化自然界中的关键反应，并可用于开发新的和可持续的生物混合电活性界面，II）蛋白质-核酸和核酸-核酸复合物结构组织过程中复杂轨迹的机理研究。该共焦拉曼光谱仪将用于检测共振拉曼（RR）和表面增强共振拉曼（SERR）模式下的拉曼散射。这些增强模式将确保通过有针对性地选择激光波长来选择性地检测复杂生物系统中的各种发色团，并将这种选择性转移到施加到电极的各个层。后者允许耦合到电化学方法，以监测例如原位的氧化还原活性，使得能够进行氧化还原活性分子的结构-功能分析。为了能够以最有效的方式利用通过RR效应的选择性以及通过SERR的表面放大的灵敏度，该光谱仪配备了5个发射266 nm、405 nm、488 nm、532 nm和591 nm单色光的激光源，以及两个在UV和维斯范围内具有高量子效率的检测器。UV源对于选择性地探测芳香族氨基酸残基或核苷酸是必不可少的，而维斯源针对不同的含金属活性位点。SERR与电化学相结合将为波恩大学化学系提供一种新的操作光谱电化学技术，该技术将用于其他工作组的合作研究目标。

The confocal Raman Spectrometer applied for here will play a central role in experimental investigations of Bio-Spectro-Electrochemistry group, which was recently set up at the Institute for Physical and Theoretical Chemistry at the University of Bonn. The planned research work is divided into two overarching directions: I) operando studies of redox biocatalytic metalloproteins that catalyze key reactions in nature and can be harnessed for the development of new and sustainable biohybrid electroactive interfaces, II) mechanistic studies of complex trajectories during the structural organization of protein-nucleic acid and nucleic acid-nucleic acid complexes. This confocal Raman Spectrometer will be used to the detect Raman scattering in Resonance Raman (RR) and Surface Enhanced Resonance Raman (SERR) mode. These enhancement modes will ensure the selective detection of various chromophores within complex biological systems through the targeted choice of laser wavelength, and transfer of this selectivity to individual layers applied to electrodes. The latter allows the coupling to electrochemical methods in order to monitor e.g. the redox activity of such systems in situ enabling structure-function analysis of redox-active molecules. In order to be able to use the selectivity through the RR effect as well as the sensitivity through the surface amplification by means of SERR in the most efficient way, this spectrometer is equipped with 5 laser sources that emit monochromatic light at 266 nm, 405 nm, 488 nm, 532 nm and 591 nm, and two detectors with high quantum efficiency in the UV and VIS range. The UV source is essential to selectively probe aromatic amino acid residues or nucleotides, while the VIS sources target different metal-containing active sites. SERR in combination with electrochemistry will provide a new operando-spectroelectrochemical technique to the Chemistry Department of the University of Bonn that will be used for research goals in other working groups in collaboration.