Raman Confocal Spectrometer

拉曼共焦光谱仪

• 批准号: 509895886
• 金额: --
• 依托单位: Rheinische Friedrich-Wilhelms-Universität Bonn
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/509895886?language=en
• 关键词: Raman; Confocal; Spectrometer

The confocal Raman Spectrometer applied for here will play a central role in experimental investigations of Bio-Spectro-Electrochemistry group, which was recently set up at the Institute for Physical and Theoretical Chemistry at the University of Bonn. The planned research work is divided into two overarching directions: I) operando studies of redox biocatalytic metalloproteins that catalyze key reactions in nature and can be harnessed for the development of new and sustainable biohybrid electroactive interfaces, II) mechanistic studies of complex trajectories during the structural organization of protein-nucleic acid and nucleic acid-nucleic acid complexes. This confocal Raman Spectrometer will be used to the detect Raman scattering in Resonance Raman (RR) and Surface Enhanced Resonance Raman (SERR) mode. These enhancement modes will ensure the selective detection of various chromophores within complex biological systems through the targeted choice of laser wavelength, and transfer of this selectivity to individual layers applied to electrodes. The latter allows the coupling to electrochemical methods in order to monitor e.g. the redox activity of such systems in situ enabling structure-function analysis of redox-active molecules. In order to be able to use the selectivity through the RR effect as well as the sensitivity through the surface amplification by means of SERR in the most efficient way, this spectrometer is equipped with 5 laser sources that emit monochromatic light at 266 nm, 405 nm, 488 nm, 532 nm and 591 nm, and two detectors with high quantum efficiency in the UV and VIS range. The UV source is essential to selectively probe aromatic amino acid residues or nucleotides, while the VIS sources target different metal-containing active sites. SERR in combination with electrochemistry will provide a new operando-spectroelectrochemical technique to the Chemistry Department of the University of Bonn that will be used for research goals in other working groups in collaboration.

这里应用的共聚焦拉曼光谱仪将在波恩大学物理与理论化学研究所最近成立的生物光谱-电化学小组的实验研究中发挥核心作用。计划的研究工作分为两个主要方向：1)氧化还原生物催化金属蛋白的操作蛋白研究，这些金属蛋白催化自然界中的关键反应，可以用于开发新的和可持续的生物杂交电活性界面；2)蛋白质-核酸和核酸-核酸复合物结构组织过程中复杂轨迹的机理研究。该共聚焦拉曼光谱仪将用于探测共振拉曼（RR）和表面增强共振拉曼（SERR）模式下的拉曼散射。这些增强模式将确保通过有针对性地选择激光波长来选择性地检测复杂生物系统中的各种发色团，并将这种选择性转移到应用于电极的各个层。后者允许耦合到电化学方法，以便监测例如这种系统的原位氧化还原活性，从而能够对氧化还原活性分子进行结构-功能分析。为了能够最有效地利用RR效应的选择性和SERR表面放大的灵敏度，该光谱仪配备了发射266、405、488nm、532nm和591nm单色光的5个激光源，以及在紫外和可见光范围内具有高量子效率的2个探测器。紫外源是选择性探测芳香氨基酸残基或核苷酸所必需的，而VIS源则针对不同的含金属活性位点。SERR与电化学相结合将为波恩大学化学系提供一种新的operando-spectroelectrochemical技术，该技术将用于其他合作工作组的研究目标。