Membrane excitability and celluar Ca2+ homeostasis in intact muscle cells from deep sea fish salvaged during an open sea expedition in 2007: elucidating mechanismus that correlate with adaption to ambient elevated pressure at depth

2007 年一次公海探险期间打捞上来的深海鱼完整肌肉细胞的膜兴奋性和细胞 Ca2 稳态：阐明与适应深海环境高压相关的机制

• 批准号: 51176385
• 负责人: Professor Dr. Oliver Friedrich
• 金额: --
• 依托单位: Lehrstuhl für Medizinische Biotechnologie (MBT)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/51176385?language=en
• 关键词: Membrane; excitability; celluar; Ca2+; homeostasis

Life under pressure is still a mystery although 99% of the earth’s biosphere resides in the deep ocean where ambient pressure can increase to levels up to 110MPa. High pressure bioscience is a growing research field gaining more and more attention from studying the effects of high pressures on cellular functions. In the last years, many projects dealt with pressure effects on nervous system and muscle in mammals or amphibians and showed marked alterations in function under pressure. However, not much is known about mechanisms that must have evolved to enable animals that reside in the deep sea to circumvent such restrictions. Studying deep sea fish is complicated as they may not survive decompression upon salvage and can hardly be kept in aquariums. On-site experimentation during open-sea expeditions offers an alternative to study freshly salvaged samples. In the present project, the applicant aims to investigate membrane excitability and Ca2+-homeostasis in muscle samples from deep sea fish during an expedition on the German research vessel ’ Sonne‘ in July 2007 that has never been attempted in this way before. The results from such an approach might provide valuable insights into how cells functionally have separated from terrestrian counterparts to withstand high pressure.

尽管地球生物圈 99% 位于深海，那里的环境压力可高达 110 兆帕，但压力下的生命仍然是一个谜。高压生物科学是一个不断发展的研究领域，通过研究高压对细胞功能的影响而受到越来越多的关注。在过去的几年里，许多项目研究了压力对哺乳动物或两栖动物神经系统和肌肉的影响，并显示出压力下功能的显着改变。然而，对于那些必须进化才能使居住在深海的动物能够规避这些限制的机制知之甚少。研究深海鱼类很复杂，因为它们可能无法在打捞时减压，并且很难保存在水族馆中。远海探险期间的现场实验提供了研究新鲜打捞样本的另一种选择。在本项目中，申请人的目标是在 2007 年 7 月德国科考船“Sonne”号探险期间，研究深海鱼类肌肉样本中的膜兴奋性和 Ca2+ 稳态，此前从未尝试过这种方式。这种方法的结果可能会为细胞如何在功能上与陆地对应物分离以承受高压提供有价值的见解。