Influence of distraction rate on regenerate formation during femoral callus distraction in the rat model

节段性骨缺损是骨科和创伤学面临的最大挑战之一，骨转移是最有效的治疗方法。虽然骨痂牵张已经建立很久了，而且尽管新的植入物不断发展，但治疗的持续时间可以是几个月到几年，因为研磨的最佳牵张速度大约为每天1 mm。因此，本动物实验的目的是质疑在分心阶段开始和结束时的最佳分心速度。实验计划用180只雄性布朗-挪威大鼠(德国苏兹菲尔德的查尔斯河)，随机分为3组。所有动物的分心距离均为9 mm。这些组根据传输速度的不同而不同(恒定与先快，然后慢与先慢，然后快。计划进行分子(qRT-聚合酶链式反应)、放射学(μ-CT-扫描)、生化(ELISA‘s)和组织学(丙烯酸酯组织学)测试，以调查再生质量。对于不同的分析，定义了四个时间点。

Segmental bone defects represent one of the greatest challenges in orthopedics and traumatology, with bone transport being the most effective treatment method. Although callus distraction has long been established and despite the constant development of new implants, the duration of treatment can be months to years, since an optimal distraction rate of approximately 1 mm per day has been taken for grated. Therefore, the aim of this animal experiment is to question the optimal distraction rate at the beginning and at the end of the distraction phase. The experiments are planned with 180 male Brown-Norway rats (Charles River, Sulzfeld, Germany), which will be randomly divided into 3 groups. Distraction distance is 9mm for all animals. The groups differ on the basis of the transport speed (constant vs. first fast, then slow vs. first slow, then fast. Molecular (qRT-PCR), radiological (μ-CT-Scans), biochemical (ELISA’s) and histological (Acrylat-histology) tests are planned to investigate the regenerate quality. For the different analyses four time points have been defined.