Influence of distraction rate on regenerate formation during femoral callus distraction in the rat model

大鼠股骨骨痂牵张过程中牵张率对再生形成的影响

• 批准号: 513898984
• 负责人: Professor Dr. Emmanouil Liodakis
• 金额: --
• 依托单位: Klinik für Unfallchirurgie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/513898984?language=en
• 关键词: Influence; distraction; rate; regenerate; formation

Segmental bone defects represent one of the greatest challenges in orthopedics and traumatology, with bone transport being the most effective treatment method. Although callus distraction has long been established and despite the constant development of new implants, the duration of treatment can be months to years, since an optimal distraction rate of approximately 1 mm per day has been taken for grated. Therefore, the aim of this animal experiment is to question the optimal distraction rate at the beginning and at the end of the distraction phase. The experiments are planned with 180 male Brown-Norway rats (Charles River, Sulzfeld, Germany), which will be randomly divided into 3 groups. Distraction distance is 9mm for all animals. The groups differ on the basis of the transport speed (constant vs. first fast, then slow vs. first slow, then fast. Molecular (qRT-PCR), radiological (μ-CT-Scans), biochemical (ELISA’s) and histological (Acrylat-histology) tests are planned to investigate the regenerate quality. For the different analyses four time points have been defined.

节段性骨缺损是骨科和创伤学中最大的挑战之一，骨转移是最有效的治疗方法。尽管骨痂牵引已经建立了很长时间，尽管新的种植体不断发展，治疗的持续时间可以是几个月到几年，因为最佳牵引率约为每天1 mm。因此，该动物实验的目的是在牵引阶段的开始和结束时询问最佳牵引速率。计划用180只雄性Brown-Norway大鼠（Charles River，Sulzfeld，德国）进行实验，将其随机分为3组。所有动物的牵引距离均为9 mm。各组根据运输速度不同（恒定vs.先快后慢vs.先慢后快）。计划进行分子（qRT-PCR）、放射学（μ-CT-扫描）、生物化学（ELISA）和组织学（丙烯酸酯-组织学）试验，以研究再生质量。对于不同的分析，定义了4个时间点。