Live-Cell Epifluorescence Microscope

活细胞落射荧光显微镜

• 批准号: 517270163
• 金额: --
• 依托单位: Philipps-Universität Marburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/517270163?language=en
• 关键词: Live; Cell; Epifluorescence; Microscope

At the Institute of Virology of the Philipps-University Marburg several groups investigate molecular mechanisms of infection, replication, and pathogenicity of highly pathogenic viruses that affect human health, including Ebola and Marburg virus, coronaviruses (SARS, MERS, and SARS-CoV-2), influenza A virus, different flaviviruses (e.g. Dengue, Zika, and West Nile), Lassa, and Nipah virus. The institute has laboratories up to the highest biosafety level that allow the study of these viruses. Many groups in the Institute utilize, among other techniques, high-end microscopy methods to study viral replication and the effects on the infected host cells to identify host cell machineries that are critical for virus entry, replication, and egress and to elucidate pathological alterations in infected cells. Beside the analysis of fixed samples of virus-infected cells, the working groups are interested in the dynamics of the formation of virus replication compartments and assembly and release of progeny virions. In addition, the interaction with different host cell organelles is another main focus of research. To study those processes, the institute has live-cell microscopes that are available in the BSL3 and BSL4 facilities, a new Nikon Ti2-E and a Leica DM6000 FS, respectively. However, the live cell microscope in the BSL2 facility is a 13-year-old Nikon TE-200E instrument. Due to its age the instrument frequently fails, the cameras are not state of the art and spare parts for repair are no longer available. To continue the highly successful research on these important human pathogens, the instrument has to be replaced as soon as possible.

在菲利普斯大学病毒学研究所，马尔堡的几个研究小组研究了影响人类健康的高致病性病毒的感染、复制和致病性的分子机制，包括埃博拉病毒和马尔堡病毒、冠状病毒（SARS、MERS和SARS-CoV-2）、甲型流感病毒、不同的黄病毒（如登革热、寨卡和西尼罗河病毒）、拉沙病毒和尼帕病毒。该研究所拥有最高生物安全级别的实验室，可以研究这些病毒。该研究所的许多小组利用高端显微镜方法研究病毒复制和对感染宿主细胞的影响，以确定对病毒进入，复制和外出至关重要的宿主细胞机制，并阐明感染细胞的病理变化。除了分析病毒感染细胞的固定样品外，工作组还对病毒复制区室形成的动力学以及子代病毒体的组装和释放感兴趣。此外，与不同宿主细胞器的相互作用是另一个主要的研究重点。为了研究这些过程，该研究所在BSL 3和BSL 4设施中分别配备了一台新的尼康Ti 2-E和一台徕卡DM 6000 FS活细胞显微镜。然而，BSL 2设施中的活细胞显微镜是一台有13年历史的尼康TE-200 E仪器。由于其年龄的仪器经常出现故障，相机不是最先进的，维修备件不再可用。为了继续对这些重要的人类病原体进行非常成功的研究，必须尽快更换仪器。