Functional interplay of reactive metabolites in diabetic organ damage in zebrafish

反应性代谢物在斑马鱼糖尿病器官损伤中的功能相互作用

• 批准号: 517361638
• 负责人: Professor Dr. Jens Kroll
• 金额: --
• 依托单位: Abteilung Vaskuläre Biologie und Tumorangiogenese
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/517361638?language=en
• 关键词: Functional; interplay; reactive; metabolites; diabetic

Loss of glyoxalase1 (glo1) in zebrafish leads to a partial methylglyoxal increase only and was accompanied by an impaired glucose tolerance. ALDH3a1, AKR1a1a, ALDH2.1, ALDH9a1b and ALDH3b1 were compensatory upregulated in glo1 mutants and they can detoxify different reactive metabolites. By using glo1/akr1a1a, glo1/aldh3a1, glo1/aldh2.1 double mutants as well as aldh9a1b and aldh3b1 single mutants, we will now test the accumulation of reactive metabolites in the different enzyme constellation and address how this leads to organ damages and if physical exercise will alter the enzyme activity and accumulation of reactive metabolites.

斑马鱼中乙二醛酶 1 (glo1) 的缺失仅导致甲基乙二醛部分增加，并伴有葡萄糖耐量受损。 ALDH3a1、AKR1a1a、ALDH2.1、ALDH9a1b 和 ALDH3b1 在 glo1 突变体中补偿性上调，它们可以解毒不同的反应性代谢物。通过使用 glo1/akr1a1a、glo1/aldh3a1、glo1/aldh2.1 双突变体以及 aldh9a1b 和 aldh3b1 单突变体，我们现在将测试不同酶群中反应性代谢物的积累，并探讨这如何导致器官损伤，以及体育锻炼是否会改变酶活性和反应性代谢物的积累。