Contribution of the cortico-hippocampal neuro-glial network to memory processing during sleep

皮质海马神经胶质网络对睡眠期间记忆处理的贡献

• 批准号: 517536780
• 负责人: Professorin Dr. Olga Garaschuk
• 金额: --
• 依托单位: Institut für experimentelle und klinische Pharmakologie und Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/517536780?language=en
• 关键词: Contribution; cortico; hippocampal; neuro; glial

Learning of complex motor tasks modifies local network activity in the primary motor cortex (M1), is substantially rehearsed during subsequent sleep and is associated with an interregional (i.e., cortico-striato-hippocampal) reorganization of the memory trace. In addition to neurons, this process critically depends on astrocytic calcium signalling. However, the learning-dependent in vivo patterns of coordinated neuro-glial activity, involved in memory formation during subsequent wake and sleep, remain unclear. Using chronic in vivo imaging this project studies, in a brain-state specific manner, neuronal and astroglial calcium signalling in M1 and the CA1 area of the hippocampus before, during and immediately after the complex wheel learning task as well as during preceding and subsequent sleep. The framing hypothesis is that the different brain states, i.e., wakefulness, NonREM and REM sleep, are characterized by distinct activity patterns of the neuro‐glial network which are changed by prior motor learning. Specifically, we test how learning performance, underlying task-specific activity patterns and interregional local field potentials (LFPs) are influenced by acute post-training sleep and sleep deprivation, and how network activity is re-activated post-training in a similar but not identical task, requiring generalization as a form of gist abstraction. Taken together our data shall provide an unprecedented insight into the in vivo interactions between neurons and neuroglia underlying complex motor learning and its consolidation by sleep.

复杂运动任务的学习改变了初级运动皮层（M1）中的局部网络活动，在随后的睡眠中基本上被排练，并且与区域间（即，皮质-纹状体-海马）记忆痕迹的重组。除神经元外，这一过程还严重依赖于星形胶质细胞的钙信号。然而，在随后的清醒和睡眠过程中参与记忆形成的协调神经胶质细胞活动的学习依赖性体内模式仍不清楚。使用慢性体内成像，该项目研究，在大脑状态特定的方式，神经元和星形胶质细胞的钙信号在M1和海马CA 1区之前，期间和之后立即复杂的车轮学习任务，以及在之前和之后的睡眠。框架假设是不同的大脑状态，即，非快速眼动睡眠和快速眼动睡眠的特征在于神经胶质细胞网络的不同活动模式，这些活动模式被先前的运动学习改变。具体来说，我们测试学习成绩，潜在的特定任务的活动模式和区域间局部场电位（LFPs）是如何受到急性训练后睡眠和睡眠剥夺的影响，以及网络活动如何在类似但不相同的任务中重新激活训练后，需要概括作为一种形式的要点抽象。总之，我们的数据将提供一个前所未有的洞察神经元和神经胶质细胞之间的相互作用，在体内的基础复杂的运动学习和巩固睡眠。