Contribution of the Cortical Neuro-Glial Network to Memory Processing during Sleep

皮质神经胶质网络对睡眠期间记忆处理的贡献

• 批准号: 450006891
• 负责人: Professorin Dr. Olga Garaschuk
• 金额: --
• 依托单位: Institut für experimentelle und klinische Pharmakologie und Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450006891?language=en
• 关键词: Contribution; Cortical; Neuro; Glial; Network

Learning of complex motor tasks modifies local network activity in the primary motor cortex (M1), is substantially rehearsed during subsequent sleep and is associated with an interregional (i.e. cortico-striato-hippocampal) reorganization of the memory trace. At the cortical level, in addition to neurons, this process critically depends on astrocytic calcium signaling. However, the learning-dependent in vivo patterns of coordinated neuro-glial activity involved in memory formation during subsequent wake and sleep remain unclear. Using chronic in vivo imaging we will monitor, in a brain-state specific manner, neuronal and astroglial calcium signaling in M1 before, during and immediately after the complex wheel learning task as well as during preceding and subsequent sleep. We will test how learning performance, underlying task-specific activity patterns and interregional local field potentials (LFPs) are influenced by acute sleep deprivation and how network activity is re-activated post learning in a similar but not identical task, requiring generalization process, i.e., gist abstraction. Taken together our data will provide an unprecedented insight into the in vivo interactions between neurons and neuroglia underlying complex motor learning and its rehearsal by sleep.

复杂运动任务的学习会改变初级运动皮层（M1）的局部网络活动，在随后的睡眠期间进行大量排练，并与记忆痕迹的区域间（即皮质-纹状体-海马）重组相关。在皮质水平上，除了神经元之外，这个过程还严重依赖于星形胶质细胞的钙信号传导。然而，在随后的清醒和睡眠期间参与记忆形成的协调神经胶质细胞活动的学习依赖性体内模式仍不清楚。使用慢性体内成像，我们将以大脑状态特定的方式监测复杂轮学习任务之前、期间和之后以及睡眠之前和之后的 M1 中的神经元和星形胶质细胞钙信号传导。我们将测试严重睡眠剥夺如何影响学习表现、潜在的特定任务活动模式和区域间局部场电位（LFP），以及在类似但不相同的任务中学习后网络活动如何重新激活，需要泛化过程，即要点抽象。总而言之，我们的数据将为复杂运动学习及其通过睡眠进行预演的神经元和神经胶质细胞之间的体内相互作用提供前所未有的见解。