Physiological and Biochemical Study of Gastrointestinal Functions.

胃肠功能的生理生化研究。

• 批准号: 09306007
• 负责人: AOYAMA Yoritaka
• 金额: $ 20.03万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09306007/
• 关键词: 1,2-diacylglycerol; orotic acid; acetyl CoA-carboxylase ihibitor; abberant crypt foci; colon cancer; activin; DMH誘発大腸ガン; aberrant crypt foci; α-グルコシダーゼ阻害物質; ダイエタリーファイバー; 胆膵液除去; 6-ヒドロキシフラボン; apoA-IV mRNA

(1) When rats were fed an orotic acid diet, liver lipid level of rats fed a diet containing casein was significantly higher than that of a diet containing egg protein or soybean protein. The addition of an orotic acid to a diet containing casein caused to decrease liver superoxide dismutase activity, to increase serum ornithine carbamoyl transferase activity, to increase the hepatic concentration of 1,2-diacylglycerol. Liver damage by administration of an orotic acid might be due to the increased concentration of 1,2-diaclglycerol and decreased activity of hepatic superoxide dismutase.(2) 9-Oxooctadeca 10,12-dienoic acid as acetyl CoA carboxylase inhibitor from red pepper was found. Its activity was 50 fold higher as compared with the normal fatty acids.(3) Dietary beet fiber had a role to supprees the formation of the aberrant crypt foci induced by 1,2-dimethylhydrazine. Furthermore, it was found that the formation of the aberrant crypt foci induced by nu -ray irradiation was suppressed by the injection of anti-asialo GMI, but not by the addition of beet fiber.(4) Apoptosis and p53 level in epithelial cell of colon was induced by the administration of 1,2-dimethylhydrazine. Further observation was increased level of activin mRNA in colon. This is the first finding that activin is a factor to stop the cell cycle in apoptosis.

(1)当大鼠喂食乳清酸饮食时，喂食含有酪蛋白的饮食的大鼠的肝脏脂质水平显著高于喂食含有鸡蛋蛋白或大豆蛋白的饮食的大鼠的肝脏脂质水平。乳清酸添加到含有酪蛋白的饮食中导致肝脏超氧化物歧化酶活性降低，血清鸟氨酸氨基甲酰基转移酶活性增加，肝脏1，2-二酰基甘油浓度增加。乳清酸引起的肝损害可能是由于1，2-二酰基甘油浓度升高和肝超氧化物歧化酶活性降低所致。(2)从红辣椒中发现了一种乙酰辅酶A羧化酶抑制剂9-氧代十八碳10，12-二烯酸，其活性是普通脂肪酸的50倍。(3)甜菜膳食纤维对偏二甲肼诱发的异常隐窝灶的形成有抑制作用。此外，还发现，通过注射抗脱唾液酸GMI而不是通过添加甜菜纤维来抑制由nu射线照射诱导的异常隐窝灶的形成。(4)用1，2-二甲基肼诱导结肠上皮细胞凋亡和p53表达。进一步观察到结肠中激活素mRNA水平增加。这是第一次发现激活素是细胞凋亡中阻止细胞周期的因子。

项目成果

Ishizuka S: "Suppression of the number of aberrant crypt foci of rat colorectnm by ingestion of sugar beet fiber regardless of administration of anti-asialo GM" Cancer Letters. 121(1). 39-43 (1997)

Ishizuka S：“无论是否施用抗唾液酸 GM，通过摄入甜菜纤维均能抑制大鼠结肠直肠异常隐窝病灶的数量”《癌症快报》。

Sonoyama K: "Stimulating effect of ileal pancreaticobiliary secretion on ileal apolipoprotein A-IV mRNA expression in fasted rats." Biosci.Biotechnol.Biochem.61(5). 887-889 (1997)

Sonoyama K：“回肠胰胆分泌对禁食大鼠回肠载脂蛋白 A-IV mRNA 表达的刺激作用。”

Nishioka T: "Baicalen, an alpha-glucosidase inhibitor in scutellaria baicalensis." J.Nat.Prod.61 (11). 1413-1415 (1998)

Nishioka T：“Baicalen，一种黄芩中的 α-葡萄糖苷酶抑制剂。”

Watanabe,J: "9-Oxooctadeca-10,12-dienoic Acids as Acetyl-CoA Carboxylase Inhibitors from Red Pepper (Capsicum annuum L.)" Biosci.Biotechnol.Biochem.(in press). (1999)

Watanabe,J：“9-Oxooctadeca-10,12-dienoic Acids as Acetyl-CoA Carboxylase Inhibitors from Red Pepper (Capsicum annuum L.)”Biosci.Biotechnol.Biochem.（出版中）。

Watanabe J: "Isolation and Identification of Acetyl-CoA Carboxylase Inhibitors from Green Tea(Camellia sinensis)" Biosci.Biotechnol.Biochem.62(3). 532-534 (1998)

Watanabe J：“绿茶（Camellia sinensis）中乙酰辅酶A羧化酶抑制剂的分离和鉴定”Biosci.Biotechnol.Biochem.62(3)。