Function of dietary cyclodextrins on some copper proteins

目的：本研究旨在探讨日粮中添加环糊精（CD）对肝脏金属硫蛋白（MT）、铜锌超氧化物歧化酶（SOD）、铜蓝蛋白和含铜细胞色素C氧化酶mRNA水平的影响。方法：雄性Wistar大鼠，体重75或85 g，分别饲喂基础饲料、5%α-CD、5%β-CD和5%γ-CD，连续7 d。检测肝脏MT、SOD、铜蓝蛋白和细胞色素C氧化酶mRNA水平。此外，还测定了肝脏MT蛋白含量和SOD、铜蓝蛋白和细胞色素C氧化酶的活性。结果：β-CD组大鼠摄食量明显低于对照组，但体重增加无显著性差异。膳食环糊精对每100 g体重的肝脏重量没有影响。利弗铜业公司 关于我们 β-CD组大鼠肝组织中β-CD含量增加或有增高趋势。肝脏MT和Cu，Zn-SOD mRNA水平均显著升高。β-CD和γ-CD均可增加肝脏MT蛋白含量。α-CD可提高肝脏Cu，Zn-SOD活性。而β-CD有增加肝脏细胞色素C氧化酶mRNA水平的趋势，γ-CD显著增加肝脏细胞色素C氧化酶mRNA水平。日粮γ-CD显著提高肝脏细胞色素C氧化酶活性。饲料γ-CD显著增加肝脏铜蓝蛋白mRNA水平。肝脏铜含量随着β-CD的添加而增加。而肝脏中的锌则不受膳食CD的影响。结论：β-CD和γ-CD可诱导某些含铜蛋白mRNA水平的升高。此后，据推测，某些含铜酶的活性可能会发生变化。少

Objective : This study was performed to document the effects of dietary cyclodextrins (CDs) on mRNA levels of metallothionein (MT), Cu, Zn-superoxide dismutase (SOD), ceruloplasmin and cytochrome C oxidase containing copper in the liver. Furthermore, the protein content of MT, and the enzyme activity of SOD, ceruloplasmin, and cytochrome C oxidase were estimated.Methodology : Male Wistar growing rats weighing 75 or 85 g were fed the basal, 5%α-, 5%β- or 5%γ-CD diet for 7 days. mRNA levels MT, SOD, ceruloplasmin and cytochrome C oxidase were estimated in the liver. Furthermore protein content of MT and the enzyme activities of SOD, ceruloplasmin, and cytochrome C oxidase in the liver were estimated. Copper and zinc content in the liver were also measured.Results : Feeding rats with β-CD resulted in lower food intake, but the significant difference in body weight gain was not observed among groups. Dietary cyclodextrins had no effect on liver weight per 100 g body weight. Liver copper co … More ntents in the liver of rats fed the β-CD increased or tended to be higher. Hepatic mRNA levels of MT and Cu, Zn-SOD significantly increased in rats fed either β- or γ-CD. The feeding with either β- or γ-CD increased the protein contents of MT in the liver. Dietary α-CD increased the hepatic activity of Cu, Zn-SOD. While, dietary β-CD tended to increase and dietary γ-CD significantly increased hepatic mRNA levels of cytochrome C oxidase. Dietary γ-CD significantly increased hepatic activity of cytochrome C oxidase. Dietary γ-CD significantly increased hepatic mRNA levels of ceruloplasmin. Liver copper was increased by feeding the β-CD. While zinc in the liver was not changed by the dietary CDs. mRNA levels of MT in the kidney increased by feeding dietary α-,β- or γ-CDs.Conclusion : Over all results indicate that dietary β- or γ-CD induces the increase in mRNA levels of some proteins containing copper. Thereafter it is surmised that the activities of some enzymes containing copper might be changed. Less