Metabolic changes in hepatic cholesterol metabolism by dietary amino acid

膳食氨基酸对肝脏胆固醇代谢的代谢变化

• 批准号: 06660154
• 负责人: AOYAMA Yoritaka
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660154/
• 关键词: Cystine; HMG-CoA reductase; Hypercholesterolemia; LDL receptor; Serum lipoproteins; HMG-CoA reductase mRNA; ^<125>I-LDL; リポたん白質

In order to clarify the mechanism for the hypercholesterolemia induced by feeding the cystine rich diet, liver HMG-CoA reductase, liver HMG-CoA reductase mRNA,LDL receptor mRNA and, the disappearance rate of ^<125>I-LDL injected into blood were estimated. The following were found.1.Serum cholesterol was increased within 3-5 days after feeding the cystine rich diet.2.Hepatic HMG-CoA reductase activity was increased within 3 days after feeding the cystine rich diet diet.3.Hepatic HMG-CoA reductase mRNA was increased 1.6-1.8 folds after feeding the cystine rich diet for 2 days.4.LDL receptor mRNA in the liver was increased after feeding the cystine rich diet for 3 days.5.The disappearance rate from the blood of ^<125>I-LDL injected into blood was increased in the rats fed the cystine rich diet.From these results described above, it is surmised that the hypercholesterolemia induced by feeding teh cystin rich diet might be due to the enhanced synthesis of cholesterol in the liver.

为了阐明高胱氨酸饲料引起高胆固醇血症的机制，测定了肝脏HMG-CoA还原酶、肝脏HMG-CoA还原酶mRNA、LDL受体mRNA<125>和注射入血的~（131）I-LDL的消失率。结果表明：1.高胱氨酸饲料喂养3-5天后，血清胆固醇升高; 2.高胱氨酸饲料喂养3天后，肝脏HMG-CoA还原酶活性升高; 3.肝脏HMG-CoA还原酶mRNA水平升高1.6- 1.8倍，肝脏HMG-CoA还原酶mRNA水平升高1.6- 1.8倍。高胱氨酸饲料喂养2d后，肝脏LDL受体mRNA表达增加，肝脏LDL受体mRNA从<125>上述结果推测，高胱氨酸饮食引起的高胆固醇血症可能是由于肝脏中胆固醇合成增加所致。

项目成果

青山頼孝: "シスチンによる肝脂質の蓄積" 必須アミノ酸研究. No.139. 6-8 (1994)

Yoritaka Aoyama：“胱氨酸引起的肝脏脂质积聚”必需氨基酸研究第 139 期（1994 年）。

Aoyama, Y., Kojima, K.and Yoshida, A.: "Hypercholesterolemia induced by feeding a cystine rich diet." Reports of the Research Committee of Essential Amino Acids (Japan). No.142. 31-36 (1995)

Aoyama, Y.、Kojima, K. 和 Yoshida, A.：“喂养富含胱氨酸的饮食诱发高胆固醇血症。”

青山頼孝: "大豆たん白質へのシスチン添加による血清脂質、リポたん白質代謝変動" 大豆たん白質研究会会誌. 15. 81-84 (1994)

Yoritaka Aoyama：“由于在大豆蛋白中添加胱氨酸而导致的血清脂质和脂蛋白代谢的变化”，《大豆蛋白研究会杂志》15. 81-84 (1994)。

Aoyama, Y., Hisanaga, N., Nishii, T.and Yoshida, A.: "Effects of some potassium compounds on liver lipid accumulation induced by excess dietary lysine." Comparative Biochemistry and Physiology. 107A. 227-231 (1994)

Aoyama, Y.、Hisanaga, N.、Nishii, T. 和 Yoshida, A.：“某些钾化合物对过量饮食赖氨酸引起的肝脏脂质积累的影响。”

青山 頼孝: "大豆たん白質へのシスチン添加による血清リポたん白質代謝の変動" 大豆たん白質研究会会誌. 14. 60-64 (1993)

Yoritaka Aoyama：“由于在大豆蛋白中添加胱氨酸而导致的血清脂蛋白代谢的变化”，《大豆蛋白研究会杂志》14. 60-64 (1993)。