Analysis of the mechanism of eukaryotic transcription initiation and its regulation based on the TATA box-binding factor TFIID

TATA盒结合因子TFIID在转录激活机制中起着至关重要的作用。我们通过多种方法阐明了TFIID的功能和结构在真核生物转录起始及其调控中的意义。果蝇TAF_<II bbb230与转录激活因子VP162竞争结合的转录激活机制人TFIID亚基之一TAF_<II bbb100特异性相互作用的功能分析3。组蛋白折叠基序参与人TAF_<II>80和TAF_<II>224相互作用的分析。转录延伸因子S-II家族成员在睾丸生殖细胞减数分裂期间和之后的限制性表达分析5。含WD40结构基序的Schizosaccharomyces pombe 72 kDa TFIID亚基的分子遗传学分析HIV-1-Tat相互作用蛋白Tip607组蛋白乙酰转移酶活性新底物特异性的阐明。TFIID亚基WD40重复基序突变诱导的分裂酵母细胞质分裂缺陷的研究8。组蛋白乙酰转移酶选择核心组蛋白赖氨酸残基的机制假说9。GBF的分离及初步鉴定一种新型的dna结合锌指蛋白，与HIV-1启动子富含gc的结合位点结合。Tip 60对核心组蛋白中6种特定赖氨酸的体外乙酰化分析[j]。从裂糖酵母pombe中分离出与酿酒酵母SSL1相对应的TFIIH亚基，对其进行分析，发现其存在保守的结构基序

TATA box-binding factor TFIID plays a crucial role in machanisms of transcription activation. We elucidated the significance of function and structure of TFIID in eukaryotic transcription initiation and its regulation by several methods.1. Mechanism of the transcriptional activation through the competitive binding of Drosophila TAF_<II>230 and the transcriptional activator VP162. Functional analysis of specific interactions of human TAF_<II>100, one of the TFIID subunit3. Analysis of the involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>224. Analysis of restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis5. Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif6. Elucidation of novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip607. Elucidation of defect in cytokinesis of fission yeast induced by mutation in the WD40 repeat motif of a TFIID subunit8. Hypothesis of mechanism of how histone acetyltransferases select lysine residues in core histones9. Isolation and initial characterization of GBF ; a novel DNA-binding zinc finger protein that binds to the GC-rich binding sites of the HIV-1 promoter10. Analysis of acetylation of six lysines of a specific class by Tip 60 in core histones in vitro11. Analysis of TFIIH subunit, through isolation of the gene from Schizosaccharomyces pombe corresponding to that of Saccharomyces cerevisiae SSL1, reveals the presence of conserved structural motifs