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Analysis of the mechanism of eukaryotic transcription initiation and its regulation based on the TATA box-binding factor TFIID

基于TATA盒结合因子TFIID分析真核转录起始及其调控机制

基本信息

批准号：
09480159
负责人：
HORIKOSHI Masami
金额：
$ 8.38万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480159/
关键词：
TATA-box binding factor TFIID TBP Transcription regulation Transcription activation Chromatin transcription Transcriptional regulatory factor Interaction between DNA and transcription factors

项目摘要

TATA box-binding factor TFIID plays a crucial role in machanisms of transcription activation. We elucidated the significance of function and structure of TFIID in eukaryotic transcription initiation and its regulation by several methods.1. Mechanism of the transcriptional activation through the competitive binding of Drosophila TAF_<II>230 and the transcriptional activator VP162. Functional analysis of specific interactions of human TAF_<II>100, one of the TFIID subunit3. Analysis of the involvement of the histone fold motifs in the mutual interaction between human TAF_<II>80 and TAF_<II>224. Analysis of restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis5. Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif6. Elucidation of novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip607. Elucidation of defect in cytokinesis of fission yeast induced by mutation in the WD40 repeat motif of a TFIID subunit8. Hypothesis of mechanism of how histone acetyltransferases select lysine residues in core histones9. Isolation and initial characterization of GBF ; a novel DNA-binding zinc finger protein that binds to the GC-rich binding sites of the HIV-1 promoter10. Analysis of acetylation of six lysines of a specific class by Tip 60 in core histones in vitro11. Analysis of TFIIH subunit, through isolation of the gene from Schizosaccharomyces pombe corresponding to that of Saccharomyces cerevisiae SSL1, reveals the presence of conserved structural motifs

TATA盒结合因子TFIID在转录激活机制中起着至关重要的作用。我们通过多种方法阐明了TFIID的功能和结构在真核生物转录起始及其调控中的意义.果蝇TAF_ 230与转录激活因子VP 162竞争性结合的转录激活机制<II>TFIID亚基之一的人TAF_ 100的特异性相互作用的功能分析<II>3.分析组蛋白折叠基序参与人TAF_<II>80和TAF_<II>224之间的相互作用。分析减数分裂期间和之后睾丸生殖细胞中转录延伸因子S-II家族成员的限制性表达5。粟酒裂殖酵母72 kDa TFIID亚基（包含WD 40结构基序6）的三联结构的分子遗传学阐明。HIV-1-达特相互作用蛋白Tip 607的组蛋白乙酰转移酶活性的新底物特异性的阐明TFIID亚基的WD 40重复基序突变诱导的裂殖酵母胞质分裂缺陷的阐明8。组蛋白乙酰转移酶如何选择核心组蛋白中的赖氨酸残基的机制假说9。GBF的分离和初步表征;一种新的DNA结合锌指蛋白，与HIV-1启动子的GC丰富结合位点结合10。分析乙酰化的六个赖氨酸的一个特定的类别的提示60核心组蛋白在体外11。通过从裂殖酵母中分离对应于酿酒酵母SSL 1的基因，对TFIIH亚基的分析揭示了保守结构基序的存在