Analysis of the function of transcription clongation factor S-II family based on the structural analyzes

基于结构分析的转录延伸因子S-II家族功能分析

• 批准号: 07307023
• 负责人: HORIKOSHI Masami
• 金额: $ 14.85万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07307023/
• 关键词: Transcriptional regulatory factor; Basal transcription factor; Interaction between DNA and transcription factors; Transcription elongation factors; Transcriptional regulation; domain structure; S-II; RT-PCR method; TFIIB

In order to elucidate the significance of function and structure of tissue-specific transcription clongation factor S-II family, we tried to isolate the various S-II family and to analyze the structure-function relationships by several methods.1) Isolation and characterization of tissue-specific S-II family from various organs.Human testis-specific S-II was isolated through the interaction with TFIIB by two-hybrid systems and three types of tissue-specific S-II family are obtained from mouse and rat by RT-RCR method. It was found that expression of mouse testis-specific S-II is restricted to testicular germ cells during and after meiosis in the course of spermatogenesis.2) Genetical analysis of structure and function of S-II family.To elucidate the in vivo function of S-II,we tried to set up S.pombe system. Moreover, it was found that there are three other S-II family members from S.cerevisiae genomes.

为了阐明组织特异性转录克隆因子S-II家族的功能和结构的意义，我们尝试用几种方法分离不同的S-II家族并分析其结构与功能的关系：1）从不同器官中分离和鉴定组织特异性S-II家族。通过RT-RCR方法从小鼠和大鼠中获得特异性S-II家族。结果表明，小鼠睾丸特异性S-II基因在精子发生过程中的表达仅限于减数分裂前后的睾丸生殖细胞。2）S-II基因家族结构与功能的遗传学分析为了阐明S-II基因的体内功能，我们尝试建立粟酒裂殖酵母系统。此外，发现存在来自酿酒酵母基因组的其他三个S-II家族成员。

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