Analysis of the function of transcription clongation factor S-II family based on the structural analyzes

为了阐明组织特异性转录延长因子S-II家族的功能和结构意义，我们尝试分离不同的S-II家族，并通过多种方法分析其结构-功能关系。1)各器官组织特异性S-II家族的分离与鉴定。通过双杂交系统与TFIIB相互作用分离出人睾丸特异性S-II，并通过RT-RCR方法从小鼠和大鼠中获得3种组织特异性S-II家族。在精子发生过程中，小鼠睾丸特异性S-II的表达仅限于睾丸生殖细胞。2) S-II家族结构与功能的遗传分析。为了阐明S-II在体内的功能，我们尝试建立S.pombe系统。此外，从酿酒酵母基因组中还发现了3个S-II家族成员。

In order to elucidate the significance of function and structure of tissue-specific transcription clongation factor S-II family, we tried to isolate the various S-II family and to analyze the structure-function relationships by several methods.1) Isolation and characterization of tissue-specific S-II family from various organs.Human testis-specific S-II was isolated through the interaction with TFIIB by two-hybrid systems and three types of tissue-specific S-II family are obtained from mouse and rat by RT-RCR method. It was found that expression of mouse testis-specific S-II is restricted to testicular germ cells during and after meiosis in the course of spermatogenesis.2) Genetical analysis of structure and function of S-II family.To elucidate the in vivo function of S-II,we tried to set up S.pombe system. Moreover, it was found that there are three other S-II family members from S.cerevisiae genomes.