Analysis of the function of transcription clongation factor S-II family based on the structural analyzes

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In order to elucidate the significance of function and structure of tissue-specific transcription clongation factor S-II family, we tried to isolate the various S-II family and to analyze the structure-function relationships by several methods.1) Isolation and characterization of tissue-specific S-II family from various organs.Human testis-specific S-II was isolated through the interaction with TFIIB by two-hybrid systems and three types of tissue-specific S-II family are obtained from mouse and rat by RT-RCR method. It was found that expression of mouse testis-specific S-II is restricted to testicular germ cells during and after meiosis in the course of spermatogenesis.2) Genetical analysis of structure and function of S-II family.To elucidate the in vivo function of S-II,we tried to set up S.pombe system. Moreover, it was found that there are three other S-II family members from S.cerevisiae genomes.