Development of Continuous Production Process for Anti-Cancer Drug by Plant Cell Culture with Medium Recycling

培养基回收植物细胞培养抗癌药物连续生产工艺开发

• 批准号: 09555249
• 负责人: SEKI Minoru
• 金额: $ 3.26万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09555249/
• 关键词: Plant Cell Culture; Taxus sp.; Taxol; Paclitaxel; Anti-Cancer Drug; Immobilization; Bioreactor; Bioprocess Engineering; タキソ-ル; 培地循環

Medium recycling as an operational strategy to increase secondary metabolite formation in plant cell culture was investigated for an anti-cancer drug, paclitaxel (Taxol【○!R.】) production using cultured cells of three Taxus spp. Using a continuous production system, paclitaxel productivity was increased by a factor of ten compared to batch operation. The continuous production of paclitaxel by immobilized cells was successfully continued for up to 40 days. A novel paclitaxel production process with medium recycling using plant cell culture of Taxus spp. is proposed. For paclitaxel recovery, the extraction with organic solvent and the adsorption with hydrophobic resins were coupled to the medium-recycling perfusion-culture system for paclitaxel production, which increased the paclitaxel productivity.

研究了抗癌药物紫杉醇（Taxol[○！R.]）使用三种红豆杉属的培养细胞生产。使用连续生产系统，紫杉醇生产率与分批操作相比提高了十倍。通过固定化细胞成功地连续生产紫杉醇长达40天。以红豆杉细胞为原料，采用培养基循环法生产紫杉醇。本文提出在紫杉醇回收方面，将有机溶剂萃取和疏水性树脂吸附耦合到紫杉醇生产的培养基循环灌注培养系统中，提高了紫杉醇的产率。

项目成果

Minoru Seki and Shintaro Furusaki: "Continuous Taxol Production with Medium Recycling"Book of Abstracts for 213rd ACS National Meeting, San Francisco. vol. 1. AGFD039 (1997)

Minoru Seki 和 Shintaro Furusaki：旧金山第 213 届 ACS 全国会议的“连续紫杉醇生产与介质回收”摘要书。

Minoru Seki, T. Ono and Shintaro Furusaki: "Comparison of Taxol productivity by Several Taxus Species"Chemical Engineering Symposium Series. 57. 161-164 (1997)

Minoru Seki、T. Ono 和 Shintaro Furusaki：“几种红豆杉属物种的紫杉醇生产力比较”化学工程研讨会系列。

Minoru Seki and Shintaro Furusaki: "Plant cell and Tissue Culture for the Production of Food Ingredients"Kluwer Academic/Plenum Press,New York. 300 (1999)

Minoru Seki 和 Shintaro Furusaki：“用于食品配料生产的植物细胞和组织培养”Kluwer 学术/Plenum 出版社，纽约。

Minoru Seki and Shintaro Furusaki: "14. Medium Recycling as an Operational Strategy to Increase Plant Secondary Metabolite Formation in Plant Cell and Tissue Culture for the Production of Food Ingredients, ed. by Fu, Singh and Curtis"Kluwer Academic/Plenu

Minoru Seki 和 Shintaro Furusaki：“14. 培养基回收作为一种操作策略，以增加植物细胞和组织培养中植物次生代谢物的形成，用于食品配料的生产，由 Fu、Singh 和 Curtis 编辑”Kluwer 学术/Plenu

Minoru Seki et al.: "Taxol Production using free and Immobilized cells of Taxus cuspidata"Biotechnology and Bioengineering. 53. 214-219 (1997)

Minoru Seki 等人：“使用东北红豆杉的游离和固定细胞生产紫杉醇”生物技术和生物工程。