Development of Continuous Production Process for Anti-Cancer Drug by Plant Cell Culture with Medium Recycling

以紫杉醇(Taxol[0！R])为研究对象，研究了在植物细胞培养中增加次生代谢物形成的操作策略--基质循环利用。使用连续生产系统，紫杉醇的生产率比间歇操作提高了10倍。固定化细胞连续生产紫杉醇成功地持续了40天。利用红豆杉植物细胞培养生产紫杉醇的新工艺。是提议的。为了回收紫杉醇，将有机溶剂提取和疏水树脂吸附相结合，建立了紫杉醇生产的介质循环灌流培养系统，提高了紫杉醇的产率。

Medium recycling as an operational strategy to increase secondary metabolite formation in plant cell culture was investigated for an anti-cancer drug, paclitaxel (Taxol【○!R.】) production using cultured cells of three Taxus spp. Using a continuous production system, paclitaxel productivity was increased by a factor of ten compared to batch operation. The continuous production of paclitaxel by immobilized cells was successfully continued for up to 40 days. A novel paclitaxel production process with medium recycling using plant cell culture of Taxus spp. is proposed. For paclitaxel recovery, the extraction with organic solvent and the adsorption with hydrophobic resins were coupled to the medium-recycling perfusion-culture system for paclitaxel production, which increased the paclitaxel productivity.