Development of Continuous Production Process for Anti-Cancer Drug by Plant Cell Culture with Medium Recycling

研究了抗癌药物紫杉醇（Taxol[○！R.]）使用三种红豆杉属的培养细胞生产。使用连续生产系统，紫杉醇生产率与分批操作相比提高了十倍。通过固定化细胞成功地连续生产紫杉醇长达40天。以红豆杉细胞为原料，采用培养基循环法生产紫杉醇。本文提出在紫杉醇回收方面，将有机溶剂萃取和疏水性树脂吸附耦合到紫杉醇生产的培养基循环灌注培养系统中，提高了紫杉醇的产率。

Medium recycling as an operational strategy to increase secondary metabolite formation in plant cell culture was investigated for an anti-cancer drug, paclitaxel (Taxol【○!R.】) production using cultured cells of three Taxus spp. Using a continuous production system, paclitaxel productivity was increased by a factor of ten compared to batch operation. The continuous production of paclitaxel by immobilized cells was successfully continued for up to 40 days. A novel paclitaxel production process with medium recycling using plant cell culture of Taxus spp. is proposed. For paclitaxel recovery, the extraction with organic solvent and the adsorption with hydrophobic resins were coupled to the medium-recycling perfusion-culture system for paclitaxel production, which increased the paclitaxel productivity.