Enhancement of Somatic Embryogenesis by a Novel Control Method of Phytohormone

通过植物激素的新控制方法增强体细胞胚胎发生

• 批准号: 09650867
• 负责人: SEKI Minoru
• 金额: $ 2.11万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650867/
• 关键词: Plant Cultured Cells; Phytohormone; Auxin; 2,4-D; Riboflavin; Somatic Embryo; Bioreactor; Bioprocess Engineering; 2.4-D

In this research, somatic embryogenesis was investigated for the enhanced regeneration efficiency from the engineering viewpoints.1) Effects of riboflavin (RF) addition in the culture systema) Decomposition rate of 2,4-D, a synthetic auxin, by riboflavin depended greatly on light irradiation intensity. FAD and EMN also showed the catalytic activity for the 2,4-D degradation, but lumichrome, the main product of 2,4-D decomposition, cannot.b) Riboflavin addition into carrot cells suspension culture increased the decrease rate of 2,4-D in the cells and the medium, also enhanced the regeneration efficiency.c) Riboflavin immobilized beads (RF-beads) were synthesized with a hydrophobic alkyl chain as a linker for the auxin decomposition.d) The carrot cell culture with RF-beads increased the efficiency of somatic embryo formation, however the excess amount of the beads inhibited the regeneration probably because the damage to the cells by activated oxygen generated through the 2,4-D degradation.c) A novel culture system with an auxin degradation vessel is proposed for the effective somatic embryogenesis.2) Analysis of gas-phase composition and gas-exchanging methoda) Effects of gas components in flask headspace of carrot cell cultures were investigated for somatic cmryogcnesis. Inhibition of ethylene gas and the suppressive effect of carbon dioxide gas on it were suggested.b) In the headspace of rice callus suspension culture, a small amount of ethanol and acetaldehyde etc. was detected, which could be an inhibitory factor for embryogenesis. However, there could not be observed any substances in the headspace gas that enhance embyogenesis.

在这项研究中，从工程学的角度研究了体细胞胚发生，以提高再生效率。1）核黄素在培养系统中添加的核黄素（RF）添加的影响2,4-D的分解速率为2,4-D，一种合成的生长素，核黄素，核黄素对光照射强度极大地依赖于光照射强度。 FAD and EMN also showed the catalytic activity for the 2,4-D degradation, but lumichrome, the main product of 2,4-D decomposition, cannot.b) Riboflavin addition into carrot cells suspension culture increased the decrease rate of 2,4-D in the cells and the medium, also enhanced the regeneration efficiency.c) Riboflavin immobilized beads (RF-beads) were synthesized with a hydrophobic烷基链作为生长素分解的连接器。d）带有RF珠的胡萝卜细胞培养提高了体细胞形成的效率，但是珠子的过量抑制了珠子的限制可能是因为再生的细胞对细胞的损害可能是通过2,4-D DEGRADITATION产生的活性氧气的氧化型，该胚胎是一种启动的动量。C）提出了一个新型培养基。研究气相组成和气体交换方法）研究了胡萝卜细胞培养物烧瓶顶空气体成分的作用，以进行体细胞cmryogcnesis。建议抑制乙烯气体及其对二氧化碳气体的抑制作用。但是，无法观察到顶空气中的任何物质可以增强弹性。