Collaborative Research: Metabolic Manipulation of Taxus sp. Cell Cultures for Taxane Production

合作研究：红豆杉的代谢调控。

• 批准号: 9625405
• 负责人: Michael Shuler
• 金额: --
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9625405&HistoricalAwards=false
• 关键词: Collaborative; Research; Metabolic; Manipulation; Taxus

9625405 Shuler The Principal Investigators (PIs) propose to fill the need for a more rational approach to optimization of production conditions for new compounds derived from plant cell cultures. They plan to exploit evolving knowledge of signal- transduction pathways in plants with experiments using metabolic transport inhibitors, precursors, and signaling compounds (e.g. elicitors and plant hormones). This approach is to lead to working models of TAXOL synthesis, release, and degradation in Taxus sp. cell cultures. The PIs believe the proposed approach should be generalizable to compounds other than TAXOL that are derived from plant cell cultures, and provide biochemical engineers a tool to achieve rapid production of novel therapeutic compounds from plant cells. ***

9625405 Shuler首席研究员（pi）提出了一种更合理的方法来优化从植物细胞培养中提取的新化合物的生产条件。他们计划利用代谢运输抑制剂、前体和信号化合物（如激发子和植物激素）的实验来开发植物信号转导途径的不断发展的知识。该方法旨在建立红豆杉细胞培养中紫杉醇合成、释放和降解的工作模型。pi认为，所提出的方法应该推广到从植物细胞培养中提取的紫杉醇以外的化合物，并为生化工程师提供一种工具，以实现从植物细胞中快速生产新的治疗性化合物。＊＊＊