Construction of Micro/Nano-Reactor Array Using Low-Volume Liquid Dispenser

利用小容量液体分配器构建微纳反应器阵列

• 批准号: 16310101
• 负责人: SEKI Minoru
• 金额: $ 10.05万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310101/
• 关键词: Microfluidic Device; Micro; Nano Dispenser; Micro-reactor Array; Microfluidics; Polydimethylsiloxane; Accumulation of Micro-particles; Contact Angle; Local Surface Modification; シランカップリング

The purpose of this research is to develop a micro-dispenser that enables the quantitative handling with multiple ultra-low volume liquid in a simple procedure and to construct a micro/nano reactor array by multiplying the developed dispensers with a volume of nano-liter to pico-liter. The main results are as follows :1.Qualification of Developed Micro Liquid DispenserIn this research, we have proposed a novel dispensing principle for a low-volume liquid, that is, a simple micro-channel network without any mechanical elements. This microchannel structure has both the narrow sections that function as a fluid valve and the multiple branching points that can quantitatively meter the small liquid volume. The former valve-like structure is generally called as a "passive valve", that requires the narrow and hard-wet region, i.e., the contact angle of the handling liquid for the channel surface is more than 90 degree. It has revealed that our newly proposed channel structure has a clear valve … More function either for a wettable or hard to wet liquid, even though that depends on the fabrication accuracy.2.Local Surface Modification for the Dispenser ImprovementThe surface modification of the channel inner wall improves the valve performance, especially for the narrow region of the dispenser. For this purpose, various silane coupling reagents were effective for PDMS microchannel modification. We have also proposed a novel local surface modification method utilizing the accumulation of small polymer particles at the specific area on a microchannel inner wall and the melting of the particles after the accumulation.3.Construction of Micro/Nano Reactor Array with Multiple Micro-DispensersWe have also developed the various types of micro/nano reactor arrays by integrating multiple micro-dispensers, that are, a 2D-microreactor array that enables the three droplets mixing at each chamber for the multiple drug resistance screening, a micro-gel compartment array using Ca-alginate for cell screening, and a chamber array that easily prepares the concentration gradient by exchanging two kinds of liquids. Less

本研究的目的是开发一种微分配器，能够在一个简单的程序中定量处理多种超低容量液体，并通过将开发的分配器与纳升至皮升的体积相乘来构建微/纳反应器阵列。主要研究结果如下：1.微型液体分配器的研制在本研究中，我们提出了一种新的小容量液体分配原理，即一个简单的微通道网络，没有任何机械元件。该微通道结构具有用作流体阀的狭窄部分和可以定量计量小液体体积的多个分支点。前一种阀状结构通常被称为“被动阀”，其需要狭窄且硬湿的区域，即，处理液体与通道表面的接触角大于90度。结果表明，我们新提出的通道结构具有清晰的阀门 ...更多信息 用于润湿或难以润湿的液体，即使这取决于制造精度。2.用于分配器的局部表面改性通道内壁的表面改性改善了阀的性能，特别是对于分配器的狭窄区域。为此，各种硅烷偶联剂是有效的PDMS微通道改性。我们还提出了一种新的局部表面改性方法，利用小聚合物颗粒在微通道内壁上特定区域的积聚和积聚后颗粒的熔融。3.具有多个微分配器的微/纳米反应器阵列的构建我们还通过集成多个微分配器开发了各种类型的微/纳米反应器阵列，即，一个二维微反应器阵列，使三个液滴在每个腔室混合，用于多重耐药性筛选，一个微凝胶隔室阵列，使用海藻酸钙细胞筛选，和一个腔室阵列，很容易通过交换两种液体制备浓度梯度。少