Preparation of a model system for biological solutions and its industrial applications

生物溶液模型系统的制备及其工业应用

• 批准号: 09650995
• 负责人: KINOSHITA Takatoshi
• 金额: $ 1.92万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650995/
• 关键词: amphiphilic; polypeptides; poly (ethylene glycol); micelles; solubilization behaviors; orderly packed micelles; plane solutes; model system for biological solutions; ポリペプチド

Amphiphilic polypeptides, poly( gamma-methyl L-glutamate)-block-poly(ethylene glycol), PMLG-PEG, and poly(gamma -benzyl L-glutamate)-block-poly(ethylene glycol), PBLG-PEG, were prepared and their aggregation structure and solubilization behaviors were investigated. These amphiphilic polypeptides were soluble in water to form spherical aggregates in which the hydrophobic alpha -helical peptide tail was orderly packed in the hexagonal manner. The solubilization of a plane solute, 1-naphthol, into these aggregates was characterized by semi- equilibrium dialysis (SED) method. Values of the solubilization equilibrium constant(K) decrease as the mole fraction of 1-naphthol in the aggregate (X) increases, And the solubilization isotherm of PBLG-PEG-1-naphthol system is higher as compared with that of PMLG-PEG-1-naphthol system. That is, the aromatic-aromatic interaction in the former system may enhance the solubilization affinity between the aggregate and solute. It is shown, therefore, that the interaction between peptide side chains and solutes is the effective factor to control the solubilization behavior of micellar aggregates composed of peptide-based amphiphiles. Thus, we can confirm the selective solubilization behaviors by the peptide- based orderly packed micelles in aqueous solutions, which may be a simple model system of our blood solutions containing albumin for their anomalous solubilization behaviors.

制备了两亲性多肽聚谷氨酸γ-甲酯-聚乙二醇嵌段共聚物（PMLG-PEG）和聚谷氨酸γ-苄酯-聚乙二醇嵌段共聚物（PBLG-PEG），并研究了它们的聚集态结构和增溶行为。这些两亲性多肽可溶于水以形成球形聚集体，其中疏水性α-螺旋肽尾以六边形方式有序地堆积。用半平衡透析法研究了平面溶质1-萘酚对这些聚集体的增溶作用. PBLG-PEG-1-萘酚体系的增溶平衡常数K值随聚集体中1-萘酚摩尔分数X的增大而减小，且PBLG-PEG-1-萘酚体系的增溶等温线高于PMLG-PEG-1-萘酚体系。也就是说，在前一个系统中的芳香族-芳香族相互作用可以增强聚集体和溶质之间的增溶亲和力。因此，肽侧链与溶质之间的相互作用是控制肽基两亲物组成的胶束聚集体增溶行为的有效因素。因此，我们可以证实肽基有序堆积胶束在水溶液中的选择性增溶行为，这可能是我们的血液溶液中含有白蛋白的异常增溶行为的一个简单的模型系统。

项目成果

T.Kinoshita: "“Photoresponsive Membrane Systems"" Photochem.Photobiol., B : Biology. 42・1. 12-19 (1998)

T.Kinoshita：“光响应膜系统”Photochem.Photobiol.，B：生物学42・1（1998）。

A.Toyotama, s.Kugimiya, M.Yonese, T.Kinoshita and Y.Tsujita: ""Controllable Orientation of the Peptide-based Surfactant at Air-Water Interface"" Chem.Lett.No.5. 443-444 (1997)

A.Toyotama、s.Kugimiya、M.Yonese、T.Kinoshita 和 Y.Tsujita：“空气-水界面上基于肽的表面活性剂的可控方向”Chem.Lett.No.5。

M.Higuchi and T.Kinoshita: ""Photoresponsive behavior of self-assembling systems by amphiphilic alpha-helix with azobenzene unit"" Photochem.Photobiol., B : Biology. Vol.42, No.1. 143-150 (1998)

M.Higuchi 和 T.Kinoshita：“具有偶氮苯单元的两亲性 α 螺旋自组装系统的光响应行为” Photochem.Photobiol.，B：生物学。

H.Ihara 他6名: "Detection of Molecular-Shope Recognition for Polycyclic Aromatic Hydrocarbons by α-Helical Poly(L-Alanine)an Silica" Chem.Lett.1998・10. 963-964 (1998)

H.Ihara等6人：“利用α-螺旋聚(L-丙氨酸)二氧化硅检测多环芳香烃”Chem.Lett.1998・10(1998)。

A.Toyotama 他4名: "“Controllable Orientation of the Peptide-based Surfactant at Air-Water Interface"" Chem.Lett.1997・5. 443-444 (1997)

A.Toyotama 等 4 人：“空气-水界面上肽基表面活性剂的可控取向”，Chem.Lett.1997・5（1997）。