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Potencial of fermented milk for anti-adhesion therapy

发酵乳用于抗粘连治疗的潜力

基本信息

批准号：
09660284
负责人：
TOBA Takahiro
金额：
$ 1.79万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660284/
关键词：
Lactobacillus crispatus S-layer protein gene amino acid sequence adhesion anti-adhesion therapy laminin enetotoxigenic Escherichia coli

项目摘要

Before this project had started, we had shown that Lactobacillus crispatus JCM 5810 well adhered to submucosal model and its 43-kDa S-layer protein(CbsA) had been identified as an adhesin.In the year 1997 primary structure of CbsA was determined by DNA sequencing. Alignment of CbsA and S-layer protein(SIpA) of non-adhesive Lactobacillus acidophilus 1CM 1132(=ATCC 4356) indicated that signal sequence of 30 amino acids was identical except one residue. The identity of two proteins was 77% in C-terminal sequence and 30% in N-terminal region. These results suggested binding region might be located in the N-terminal region.In the year 1998 deletion and point mutation was incorporated into CbsA gene and the expressed His-tag fusion protein was evaluated for binding to type IV collagen. The results suggested that 1-2 87 amino acid sequence was necessary to bind type IV collagen. Lb. crispatus JCM 5810 and its S-layer protein inhibited the adherence of enterotoxigenic Escherichia coli on Matrigel and immobilized laminin, used as an submucosal model.The above results suggested S-layer-bearing lactobacillar strains and their S-layer protein have the potential to apply anti-adhesion theraputic agent for intestinal infection disease to inhibite collonization of pathogens.

在这个项目开始之前，我们已经证明卷曲乳杆菌JCM 5810能够很好地粘附在粘膜下模型上，并且它的43-kDa S层蛋白（CbsA）已被鉴定为粘附素。1997年通过DNA测序确定了CbsA的一级结构。非粘附性嗜酸乳杆菌1CM 1132(=ATCC 4356)的CbsA和S层蛋白(SIpA)的比对表明，除1个残基外，30个氨基酸的信号序列相同。 The identity of two proteins was 77% in C-terminal sequence and 30% in N-terminal region.这些结果表明结合区域可能位于N末端区域。1998年，CbsA基因中被引入缺失和点突变，并评估表达的His标签融合蛋白与IV型胶原的结合。 The results suggested that 1-2 87 amino acid sequence was necessary to bind type IV collagen.磅。卷曲菌 JCM 5810 及其 S 层蛋白抑制产肠毒素大肠杆菌在基质胶和固定化层粘连蛋白上的粘附，用作粘膜下模型。上述结果表明带有 S 层的乳杆菌菌株及其 S 层蛋白有潜力应用于肠道感染疾病的抗粘附治疗剂以抑制定植病原体。