Laminar Bone Foromation and Comparative Histology on Osteon-Remodeling in Some Species of Aritidactla

一些拟南芥属的板层骨形成和骨重塑的比较组织学

基本信息

批准号：
09670030
负责人：
MORI Ryoichi
金额：
$ 1.15万
依托单位：
Showa University School of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670030/
关键词：
Artiodactyla laminar bone bright line woven bone lamellar bone appearance of osteon comparative histology scanning electron microscopy 歯状骨四肢長骨組織学走査電顕・分析電顕骨形成ハバース層板骨

项目摘要

We investigated the limb long-bone diaphyses of various-aged bovines, goats, pigs, and sheep by transmitted light, scanning electron, transmitted elect'ron microscopy, and enegy-dispersive X-ray microanalysis.In a 3-rnonthfetus bovine, 3-4 concentric layers of laminar bone appeared around the bone marrow and 25-26 layers were seen in a new-born calf. In a 1-year-old ox, osteons appeared in the inner-third zone, however, the laminar bone was retained yet in a 12-year-old cow. The hypercalcified center of laminar bone, so-called a bright line, scattered relatively large granular and fine fibrillar structures. After formation of this line, woven bone and lamellar bone were formed on the line. The appositional volume was larger in the endosteum than the periosteum side. There were a few osteocytes in the bright line, but the cell lacunae were larger than the normal lacunae. Thus, the formation of bone matrix may somewhat differ from each other. From the bright line, larger amounts of Na and S elements were detected.In Van-Gieson staining for collagen fibrils, the bright line showed no staining. In H-E and AZAN staining, the line was distinguishable from the woven and lamellar bones, and also differed from them in the younger laminar bone stained with toluidin blue. However, we can not identified the organic elements of the granular and fibrillar structures found in the bright line.In the formation of limb-long bones in young goats, pigs, and sheep, laminar bone similar to that of bovines was also observed. The laminar bone was rapidly formed and osteons that should form Haversian bone did not appear in the laminar bone for a certain period of time after birth. The appearance was earlier seen in pigs and latest in bovines as a large animal.

我们通过传播光线，扫描电子，传输的选举显微镜和eNegy-dispertive X射线微分析的各种牛，山羊，猪和绵羊的肢体长骨diaphys。小牛。然而，在一只1岁的牛中，骨出现在内部第三区，但是，层状骨头仍保留在一只12岁的牛中。层状骨的超钙化中心，所谓的明亮线，相对较大的颗粒和细纤维结构。形成这条线后，在线上形成了编织的骨头和层状骨。在内接和骨膜侧，围位体积大于骨膜侧。明亮的线中有几个骨细胞，但是细胞空隙大于正常的lacunae。因此，骨基质的形成可能彼此有些不同。从明亮的线中，检测到大量的Na和S元素。在胶原原纤维的Van-Gieson染色中，明亮的线没有染色。在H-E和Azan染色中，该线与编织和层状骨骼可区分，并且在用甲苯甲蛋白蓝色染色的年轻层状骨中也有所不同。但是，我们无法鉴定出在明亮的线中发现的颗粒状和原纤维结构的有机元素。在幼山羊，猪和绵羊的肢体长骨形成中，还观察到了类似于牛的层骨。层状骨迅速形成，骨骨应在出生后的一段时间内出现在层状骨中。该外观早些时候在猪和最新的牛看来是一种大动物。