Laminar Bone Foromation and Comparative Histology on Osteon-Remodeling in Some Species of Aritidactla

对不同年龄的牛、山羊、猪和绵羊的四肢长骨骨干进行了透射光、扫描电子显微镜、透射电子显微镜和X射线能谱显微分析。在一头1岁的牛身上，内第三区出现了骨质，然而，在一头12岁的牛身上，板状骨仍然保留着。钙化中心的板层状骨，即所谓的亮线，散布着较大的颗粒状和细小的纤维状结构。该线形成后，形成编织骨和板层骨。骨内膜的相对体积大于骨膜侧。亮线内有少量骨细胞，但细胞陷窝较正常陷窝大。因此，骨基质的形成可能会有所不同。从亮线中检测到较多的Na和S元素，而在胶原纤维的Van-Gieson染色中，亮线未见染色。在H-E和Azan染色中，这条线与编织骨和板层骨相区别，也不同于甲苯胺蓝染色的较年轻的板层骨。在幼年山羊、猪和绵羊四肢长骨的形成过程中，也观察到了与牛相似的片状骨。板层骨形成迅速，出生后一段时间内未出现本应形成哈弗斯骨的骨小节。这种现象最早出现在猪身上，最近出现在牛身上，是一种大型动物。

We investigated the limb long-bone diaphyses of various-aged bovines, goats, pigs, and sheep by transmitted light, scanning electron, transmitted elect'ron microscopy, and enegy-dispersive X-ray microanalysis.In a 3-rnonthfetus bovine, 3-4 concentric layers of laminar bone appeared around the bone marrow and 25-26 layers were seen in a new-born calf. In a 1-year-old ox, osteons appeared in the inner-third zone, however, the laminar bone was retained yet in a 12-year-old cow. The hypercalcified center of laminar bone, so-called a bright line, scattered relatively large granular and fine fibrillar structures. After formation of this line, woven bone and lamellar bone were formed on the line. The appositional volume was larger in the endosteum than the periosteum side. There were a few osteocytes in the bright line, but the cell lacunae were larger than the normal lacunae. Thus, the formation of bone matrix may somewhat differ from each other. From the bright line, larger amounts of Na and S elements were detected.In Van-Gieson staining for collagen fibrils, the bright line showed no staining. In H-E and AZAN staining, the line was distinguishable from the woven and lamellar bones, and also differed from them in the younger laminar bone stained with toluidin blue. However, we can not identified the organic elements of the granular and fibrillar structures found in the bright line.In the formation of limb-long bones in young goats, pigs, and sheep, laminar bone similar to that of bovines was also observed. The laminar bone was rapidly formed and osteons that should form Haversian bone did not appear in the laminar bone for a certain period of time after birth. The appearance was earlier seen in pigs and latest in bovines as a large animal.