Assessment of human myocardial metabolism by nuclear magnetic resonance spectroscopy using proton excitation

使用质子激发的核磁共振波谱评估人体心肌代谢

• 批准号: 09670712
• 负责人: MITSUNAMI Kenichi
• 金额: $ 2.05万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670712/
• 关键词: nuclear magnetic resonance spectroscopy; human myocardium; proton MRS; STEAM method; PRESS method; creatine; プロトン・デカップリング; 核オーバーハウザ-効果; リンMRS

Proton (ィイD11ィエD1H) nuclear magnetic resonance (NMR) spectroscopy (MRS) was performed on a clinical (1.5-T) magnetic-resonance-imaging system. A receive-only flexible surface coil was placed over the left ventricle with subjects in the supine position. Voxels were localized to 8 ml with stimulated echo acquisition mode (STEAM) or point-resolved spectroscopy (PRESS) at a repetition time of 1.5 s to keep relaxation distortions to a minimum. We collected 8 free induction decays (FID) for water signal without water suppression before acquiring 256 water-suppressed FID's for creatine signal. Total creatine was identified and measured based on its N-methyl resonance at around 3.0 parts per million (ppm) taking water signal as a reference at 4.75 ppm. To measure total myocardial creatine we used the tissue-water signal in the unsuppressed ィイD11ィエD1H MRS spectrum as a concentration reference. Normalization of creatine signal by the water signal from the same voxel eliminates the systematic effects that result from differences in voxel size, location, and heterogeneity in the receiver-coil reception. Creatine resonance appeared at 3.06±0.07 ppm by STEAM method and at 3.18±0.07 ppm by PRESS. Total myocardial creatine content in a scarred interventricular septum was 25.7 μmol/g wet weight by STEAM. These findings suggest that quantitative human myocardial ィイD11ィエD1H MRS is feasible both by STEAM and by PRESS and that the two methods yield similar myocardial creatine content values.

在临床（1.5-T）磁共振成像系统上进行质子（H2O D11 H2O D1 H）核磁共振（NMR）光谱（MRS）。将仅接收柔性表面线圈放置在受试者仰卧位的左心室上。以1.5 s的重复时间用受激回波采集模式（STEAM）或点分辨光谱（PRESS）将体素定位到8 ml，以使弛豫失真保持最小。我们收集了8个无水抑制的水信号的自由感应衰减（FID），然后获得256个肌酸信号的水抑制FID。总肌酸基于其N-甲基共振在约百万分之3.0（ppm）下鉴定和测量，以4.75ppm下的水信号作为参考。为了测量总的心肌肌酸，我们使用未抑制的肌酸激酶D11肌酸激酶D1 H MRS频谱中的组织水信号作为浓度参考。通过来自相同体素的水信号对肌酸信号进行归一化消除了由体素大小、位置和接收器线圈接收中的异质性的差异引起的系统效应。STEAM法和PRESS法分别在3.06±0.07 ppm和3.18±0.07 ppm处出现肌酸共振。STEAM法测得瘢痕室间隔心肌肌酸总含量为25.7 μmol/g湿重。这些结果表明，定量的人心肌肌酸D11肌酸D1 H MRS是可行的两个蒸汽和压力，这两种方法产生类似的心肌肌酸含量值。

项目成果

矢部 隆宏: "ヒト心筋三次元CSIの試みと臨床応用上の問題点" 日本臨牀. 55・7. 1839-1843 (1997)

Takahiro Yabe：“人体心肌三维CSI的尝试和临床应用中的问题”Nippon Rinsho 55・7 1839-1843（1997）。

Atsushi Takaoka, Ichiro Nakae, Kenichi Mitsunami, et al.: "Renal ischemia/reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via adenosine receptors in rabbits : effects of "remote" preconditioning""Journal of American C

Atsushi Takaoka、Ichiro Nakae、Kenichi Mitsunami 等人：“肾缺血/再灌注通过兔子的腺苷受体远程改善心肌缺血期间的心肌能量代谢：“远程”预处理的效果”“美国 C 杂志

Atsushi Takaoka, Ichiro Nakae, Kenichi Mitsunami, Takahiro Yabe, Shigehiro Morikawa, Toshiro Inubushi, Masahiro Kinoshita: "Renal ischemia/reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via adenosine receptors in rab

Atsushi Takaoka、Ichiro Nakae、Kenichi Mitsunami、Takahiro Yabe、Shigehiro Morikawa、Toshiro Inubushi、Masahiro Kinoshita：“肾缺血/再灌注通过 rab 中的腺苷受体远程改善心肌缺血期间的心肌能量代谢

Atsushi Takaoka,Ichiro Nakae,Kenichi Mitsunami et al.: "Renal ischemial reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via adenosine receptors in rabbits: effects of "remote preconditioning""Journal of American Colle

Atsushi Takaoka、Ichiro Nakae、Kenichi Mitsunami 等人：“肾缺血再灌注通过腺苷受体远程改善兔子心肌缺血期间的心肌能量代谢：“远程预处理”的效果”美国大学杂志

Mamoru Okada, Kenichi Mitsunami, Toshiro Inubushi, Masahiko Kinoshita: "Influence of aging or left ventricular hypertrophy on the human heart : Contents of phosphorus metabolites measured by ィイD131ィエD1P MRS"Magn. Reson. Med.. 39-5. 772-782 (1998)

Mamoru Okada、Kenichi Mitsunami、Toshiro Inubushi、Masahiko Kinoshita：“衰老或左心室肥大对人类心脏的影响：通过 D131D1P MRS 测量的磷代谢物的含量”Magnet.. 772-782 (1998) ）