Mechanism of prevention of lung metastasis or sarcoma by tumor-associated macrophage

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Antitumor effect of the intra-tumoral injection of Cepharanthin<encircledR> and staphylococcal enterotoxin B was evaluated using F 344 male rats bearing transplantable rat-osteosarcoma, S-SLM.A macroscopic lung metastatic nodule of the tumor was transplanted into subcutaneous back space, and 0.5 mg Cepharanthin and 2 pg staphylococcal enterotoxin B were injected into the tumor on day 12, 13 and 14. On day 28, all animals were euthanized in overdose of pentobarbital sodium, and the transplanted tumor and lung were examined. The wet weight of the lung of the rats treated with Gepharanthin and staphylococcal enterotoxin B was significantly lower, and the number of apoptosis of the lung metastatic nodules was significantly higher than those of the control and treated with only Cepharanthin or staphylococcal enterotoxin B.In the transplanted tumors, an infiltration of TRAP (tartrate-resistant acid phosphatase)-positive multinucleated giant cells was prominent in the rats treated with Cepharanthin and staphylococcus enterotoxin B.These findings indicate that intra-tumoral injection of Cepharanthin and staphylococcal enterotoxin B induced infiltration of TRAP-positive multinucleated giant cells within the transplanted rat-osteosarcoma, and prevent their lung metastasis.