Mechanism of prevention of lung metastasis or sarcoma by tumor-associated macrophage

以f344例移植大鼠骨肉瘤（S-SLM）雄性大鼠为实验对象，观察瘤内注射头黄素<环抱dr >和葡萄球菌肠毒素B的抗肿瘤作用。将肉眼可见的肿瘤肺转移结节移植至皮下后腔，于第12、13、14天向肿瘤内注射头孢菌素0.5 mg和葡萄球菌肠毒素B 2 pg。第28天给予戊巴比妥钠过量安乐死，检查移植瘤及肺组织。与对照组和单纯头孢蓝素或单纯葡萄球菌肠毒素B组相比，应用头孢蓝素和葡萄球菌肠毒素B组肺湿重明显降低，肺转移结节细胞凋亡数量明显增加。在大鼠骨肉瘤移植瘤内注射头孢蓝素和葡萄球菌肠毒素B后，TRAP（酒石酸抗性酸性磷酸酶）阳性的多核巨细胞明显浸润，提示瘤内注射头孢蓝素和葡萄球菌肠毒素B可诱导TRAP阳性的多核巨细胞浸润，防止骨肉瘤肺转移。

Antitumor effect of the intra-tumoral injection of Cepharanthin<encircledR> and staphylococcal enterotoxin B was evaluated using F 344 male rats bearing transplantable rat-osteosarcoma, S-SLM.A macroscopic lung metastatic nodule of the tumor was transplanted into subcutaneous back space, and 0.5 mg Cepharanthin and 2 pg staphylococcal enterotoxin B were injected into the tumor on day 12, 13 and 14. On day 28, all animals were euthanized in overdose of pentobarbital sodium, and the transplanted tumor and lung were examined. The wet weight of the lung of the rats treated with Gepharanthin and staphylococcal enterotoxin B was significantly lower, and the number of apoptosis of the lung metastatic nodules was significantly higher than those of the control and treated with only Cepharanthin or staphylococcal enterotoxin B.In the transplanted tumors, an infiltration of TRAP (tartrate-resistant acid phosphatase)-positive multinucleated giant cells was prominent in the rats treated with Cepharanthin and staphylococcus enterotoxin B.These findings indicate that intra-tumoral injection of Cepharanthin and staphylococcal enterotoxin B induced infiltration of TRAP-positive multinucleated giant cells within the transplanted rat-osteosarcoma, and prevent their lung metastasis.