Ultrasound therapy for soft tissue sarcoma with new quinolone antibiotics as a sonodynamic compound

使用新型喹诺酮抗生素作为声动力化合物对软组织肉瘤进行超声治疗

• 批准号: 16591469
• 负责人: OKADA Kyoji
• 金额: $ 0.96万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591469/
• 关键词: Sparfloxacin; Ultrasound; Antitumor effect; New Quinolone Antibiotics; Sonodynamic compound; Sarcoma

To determine if there is any synergistic antitumor effect of ultrasound (US) in the presence of new quinolone (NQ) antibiotics, 0.2 mM solution of lomefloxacin hydrochloride (LFLX), sparfloxacin (SPFX), ciprofloxacin hydrochloride (CPFX), and gatifloxacin hydrate (GFLX) were used as sonodynamic compound against sarcoma 180 cells in vitro. After US irradiation at 2 W/cm^2 for 30 and 60 seconds, the survival rates of tumor cells in NQ antibiotics added groups were significantly lower than those without (P<0.001). In May-Giemsa smear, most of tumor cells were maintained intact in control group. However, in 0.2 mM SPFX added group, the tumor cells were mostly broken into pieces. The synergistic antitumor effects of SPFX were also dose-dependent. Furthermore, when D-mannitol was used with SPFX, the survival rate of tumor cells after irradiation was comparable with that when SPFX alone was applied, but when L-histidine was used concurrently, the survival rate of tumor cells was significantly higher than that when SPFX alone was applied. These findings suggest that NQ antibiotics would be useful compounds for antitumor activities under US irradiation, and generation of singlet oxygen was involved in the in cell damage process.

为了确定在新的喹诺酮（NQ）抗生素存在下超声（US）是否有任何协同抗肿瘤作用，将盐酸洛美沙星（LFLX）、司帕沙星（SPFX）、盐酸环丙沙星（CPFX）和加替沙星水合物（GFLX）的0.2mM溶液用作体外抗肉瘤180细胞的声动力化合物。2 W/cm^2超声照射30、60 s后，加NQ抗生素组肿瘤细胞存活率明显低于不加NQ抗生素组（P<0.001）。May-Giemsa涂片显示对照组肿瘤细胞大部分保持完整。然而，在添加0.2mM SPFX的组中，肿瘤细胞大多破碎成碎片。SPFX的协同抗肿瘤作用也呈剂量依赖性。此外，当D-甘露醇与SPFX联合使用时，照射后肿瘤细胞的存活率与SPFX单独使用时相当，但当L-组氨酸同时使用时，肿瘤细胞的存活率显著高于SPFX单独使用时。这些结果表明，NQ抗生素在超声辐照下具有抗肿瘤活性，单线态氧的产生参与了细胞损伤过程。

项目成果

Ultrastructure of sarcoma 180 cells after ultrasound irradiation in the presence of sparfloxacin.

• 发表时间: 2004-05
• 期刊: Anticancer research
• 影响因子: 2
• 作者: Deqing Huang;K. Okada;Chiyo Komori;E. Itoi;K. Kawamura;Toshio Suzuki