Enhanced antitumor effect of ultrasound in the presence of piroxicam in a mouse airpouch model.

在小鼠气袋模型中，在吡罗昔康存在下，超声波增强了抗肿瘤作用。

• 批准号: 11671414
• 负责人: OKADA Kyoji
• 金额: $ 1.02万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671414/
• 关键词: piroxicam; ultrasound; antitumor effect; soft tissue tumor; sarcoma; Echo; Air Pouch; Sarcoma

The antitumor effects of piroxicam, a non-steroidal anti-inflammatory drug, under ultrasonic irradiation on sarcoma 180 cells were examined in a mouse air pouch model. When piroxicam was added to sarcoma 180 suspension under the ultrasound irradiation (2 MHz, 10 W, 120 s), the mortality rate of tumor cells immediately after the irradiation and the survival rate of mice were significantly higher than those when ultrasound alone was applied, and these effects of piroxicam were dose-dependent. When Lhistidine was used with piroxicam, the mortality rate of the tumors cells after the irradiation was comparable with that when ultrasound alone was applied, and when D-mannitol was used concurrently, the antitumor effect was significantly greater than that when ultrasound alone was applied. Histological examinations of the air pouch including the surrounding tissue showed highly degenerative tumor tissue in the air pouch and normal appearance of surrounding tissue. The findings suggest that piroxicam enhances the antitumor effects of ultrasound in vivo by increasing the production of singlet oxygen without any damage to tissue surrounding the tumor.

采用小鼠气囊模型，观察了超声辐照下非甾体抗炎药吡罗昔康对肉瘤180细胞的抗肿瘤作用。吡罗昔康在2 MHz、10 W、120 s的超声辐照下加入肉瘤180悬液中，照射后即刻肿瘤细胞的死亡率和小鼠的存活率均显著高于单独超声辐照，且呈剂量依赖性。当L-组氨酸与吡罗昔康联合使用时，照射后肿瘤细胞的死亡率与单独应用超声时相当，而当D-甘露醇同时使用时，抗肿瘤作用明显大于单独应用超声时。对气囊（包括周围组织）进行的组织学检查显示，气囊中存在高度退行性肿瘤组织，周围组织外观正常。研究结果表明，吡罗昔康通过增加单线态氧的产生来增强体内超声的抗肿瘤作用，而不会对肿瘤周围组织造成任何损伤。

项目成果

Sakusabe N: "Enhanced sonodynamic antitumor effect of ultrasound in the presence of nonsteroidal anti-inflammatory drugs."Jpn.J.Cancer Res. 90. 1146-1151 (1999)

Sakusabe N：“在非甾体抗炎药存在下增强超声的声动力抗肿瘤作用。”Jpn.J.Cancer Res。

Kyoji Okada: "Low-grade intraosseous osteosarcoma in northern Japan. Advantage of AgNOR and MIB-1 staining in differential dignosis"Hum.Pathol. 31. 633-639 (2000)

Kyoji Okada：“日本北部的低度骨内骨肉瘤。AgNOR 和 MIB-1 染色在鉴别诊断中的优势”Hum.Pathol。

Kyoji Okada: "Low-grade intraosseous osteosarcoma in northern Japan Advantage of AgNOR and MIB-1 staining in differential diagnosis."Hum Pathol. 31. 633-639 (2000)

Kyoji Okada：“日本北部的低度骨内骨肉瘤 AgNOR 和 MIB-1 染色在鉴别诊断中的优势。”Hum Pathol。

Sakusabe N, Okada K, Sato K, Kamada S, Yoshida Y, Suzuki T.: "Enhanced sonodynamic antitumor effect of ultrasound in the prsence of nonsteroidal anti-inflammatory drugs."Jpn J Cancer Res. 90. 1146-1151 (1999)

Sakusabe N、Okada K、Sato K、Kamada S、Yoshida Y、Suzuki T.：“在存在非甾体抗炎药的情况下增强超声波的声动力抗肿瘤作用。”Jpn J Cancer Res。

Kyoji Okada, Jun Nishida, Tetsuro Morita, Hiroshi Kakizaki, Akira Ishikawa, Tetsuo Hotta.: "Low-grade intraosseous osteosarcoma in northern Japan : Advantage of AgNOR and MIB-1 staining in differential diagnosis."Hum Pathol. 31. 633-639 (2000)

Kyoji Okada、Jun Nishida、Tetsuro Morita、Hiroshi Kakizaki、Akira Ishikawa、Tetsuo Hotta.：“日本北部的低度骨内骨肉瘤：AgNOR 和 MIB-1 染色在鉴别诊断中的优势。”Hum Pathol。