Antitumor effect of acridine orange under ultrasonic irradiation in vitro

超声辐照下吖啶橙的体外抗肿瘤作用

• 批准号: 18591621
• 负责人: OKADA Kyoji
• 金额: $ 1.96万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591621/
• 关键词: Acridine orange; Ultrasound; Sarcoma; U1trasound; sarcoma

Background: Sonodynamic synergistic antitumor therapy is a promising new methodology for cancer treatment.Materials and Methods: To determine the antitumor effects of ultrasound (US) in the presence of acridine orange (AO), 1.0 μg/ml solution of AO was tested as a sonodynamic compound against sarcoma 180 cells in vitro.Results: After US irradiation at 2.0 W/cm^2 for 60 sec, the survival rate of tumor cells in the presence of AO was significantly lower than in its absence (p<0.001). In the AO-treated group, the tumor cells were mostly fragmented. When D-mannitol or L-histidine was used along with the AO, the survival rate of tumor cells after irradiation was significantly higher than that when AO alone was applied.Conclusion: AO could exhibit useful antitumor activity under US irradiation, and the generation of singlet oxygen and hydroxyl radicals is involved in the processes of tumor cell damage.

背景资料：声动力协同抗肿瘤治疗是一种很有前途的肿瘤治疗新方法。材料与方法：以吖啶橙子（AO）为声动力复合物，测定1.0 μg/ml AO对肉瘤180细胞的体外抗肿瘤作用。结果：AO与超声联合作用后，肿瘤细胞的生长速度明显加快。在2.0 W/cm ^2的超声照射60秒后，AO存在时肿瘤细胞的存活率显著低于AO不存在时（p<0.001）。在AO处理组中，肿瘤细胞大多是碎片。结论：AO在超声辐照下具有抗肿瘤活性，其作用机制可能与产生单线态氧和羟基自由基有关。

项目成果

Acridine Orange is one of the promising sonodynamic compounds-Clinical application for cases with huge soft tissue sarcoma

吖啶橙是一种有前景的声动力化合物——巨大软组织肉瘤的临床应用

• 发表时间: 2007
• 作者: Okada K;Suzuki N;Chida S;Komori C
• 通讯作者: Komori C

Acridine Orange is one of the promising sonodynamic compounds-Clinical application for cases with huge soft tissue sarcoma-

吖啶橙是一种有前途的声动力化合物-巨大软组织肉瘤病例的临床应用-

• 发表时间: 2007
• 作者: Okada K;Suzuki N;Chida S;Komori C.
• 通讯作者: Komori C.

Antitumor effect of acridine orange under ultrasonic irradiation in vitro.

• 发表时间: 2007-11
• 期刊: Anticancer research
• 影响因子: 2
• 作者: Norio Suzuki;K. Okada;Shuichi Chida;Chiyo Komori;Y. Shimada;Toshio Suzuki

Acridine Orange is one of the promising sonodynamic compounds - Clinical application for cases with huge soft tissue sarcoma-

吖啶橙是一种有前途的声动力化合物 - 巨大软组织肉瘤病例的临床应用 -

• 发表时间: 2007
• 作者: Okada;K;Suzuki;N;Chida;S;Komori;C.
• 通讯作者: C.