PREOPERATIVE CYTOLOGICAL DIAGNOSIS FOR BONE AND SOFT TISSUE SARCOMAS USING IN SITUHYBRIDIZATION AND IN SITUPCR.

使用原位杂交和原位 PCR 对骨和软组织肉瘤进行术前细胞学诊断。

• 批准号: 09671476
• 负责人: HOTTA Tetsuo
• 金额: $ 1.86万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671476/
• 关键词: in situ hybrisization; in situ PCR; cell culture; bone and soft tissue tumors; aspiration biopsy cytology; in situ hybridization法

1. We have developed a nonel DNA in situ. hybridization system with digoxigenin (DIG) PCR labeled DNA probes for human specific "Alu" and mouse specific "mouse L1 (m-L1)" genes. We clarified the origins of bone-forming cells in human osteosarcoma transplanted into nude mice using this system. This system also revealed the origin of histiocyte-like cells and mulinucleated giant cells in malignant fibrous histiocytoma.2. Certain chromosomal translocations involved in Ewing sarcoma and synovial sarcoma. We have established a new synovial sarcoma cell line with SYT-SSX2 translocation. Using this cell line and Ewing sarcama cell lines, we have establised in vivo tumor model for in situ hybridization and in situ PCR, We investigated these model to develop in situ hybridization and in situ PCR system for clinical application.

1.我们已经开发了一种原位导爆管DNA。用地高辛（DIG）PCR标记的人特异性“Alu”和小鼠特异性“小鼠L1（m-L1）”基因的DNA探针的杂交系统。我们利用该系统阐明了人骨肉瘤裸鼠移植瘤中成骨细胞的来源。该系统还揭示了恶性纤维组织细胞瘤中组织细胞样细胞和多核巨细胞的起源.尤文肉瘤和滑膜肉瘤涉及的某些染色体易位。我们建立了一个新的滑膜肉瘤细胞系SYT-SSX 2易位。利用该细胞系和尤文肉瘤细胞系建立了原位杂交和原位PCR的体内肿瘤模型，并对这些模型进行了研究，以建立原位杂交和原位PCR体系，为临床应用奠定基础。

项目成果

Hatano H: "The mechanism of bone formation in human osteosarcoma analyzed by in situ hybridization." Transactions of the 43rd Annual Meeting Orthopedic Research Society.USA. 22. 319 (1997)

Hatano H：“通过原位杂交分析人类骨肉瘤的骨形成机制。”

畠野 宏史: "ヒト骨肉腫における骨形成細胞の起源-腫瘍性骨形成か反応性骨形成か?-" 日本整形外科学会雑誌. 71(8). S1372- (1997)

Hiroshi Hatano：“人类骨肉瘤中骨形成细胞的起源 - 肿瘤性骨形成或反应性骨形成？”日本骨科学会杂志 71（8）。

Hatano H: "Expression of Cbfa1 gene in human malignant tumors : A specific marker of osteoblastic tumor." Transactions of the 44th Annual Meeting Orthopedic Research Society. USA. 23. 564 (1998)

Hatano H：“Cbfa1 基因在人类恶性肿瘤中的表达：成骨细胞肿瘤的特异性标志物。”

Hiroshi Hatano: "The mechanism of bone formation in human osteosarcoma analyzed by in situ hybridization" Transactions of the 43rd Annual Meeting Orthopaedic Research Society. 22. 319- (1997)

Hiroshi Hatano：“通过原位杂交分析人类骨肉瘤的骨形成机制”第43届骨科研究会年会汇刊。

Hatano H: "Expression of Cbfa1 gene in human malignant tumors : A specific marker of osteoblastic tumor." Transactions of the 44th Annual Meeting Orthopedic Research Society.USA. 23. 564 (1998)

Hatano H：“Cbfa1 基因在人类恶性肿瘤中的表达：成骨细胞肿瘤的特异性标志物。”