A study for mechanism and treatment of aseptic loosening in total hip arthroplasty -Biclogical activity of wear particles, pathoicgical role of parathyroid hormone related peptide (PTHrP) and the therapeutical effect of bisphospham

我们的第一年研究表明，PTHrP在修订版期间联合流体中的总臀部动脉是集中在正常血清中的10个或10个时间超过10个或10个时间，而假的问题，有骨骼吸收的潜在作用，是由抗PTHrP抗体保留的。由于PTHrP具有骨骼吸收的促进功能，它被强烈怀疑与PTHrP的松弛和与全髋关节炎相关的骨质疏松症。根据本研究的结果，我们计划进行第二年的实验，以介绍PHTrP的调查因素。也有许多因素与骨质疏松症有关，与全髋关节炎、聚乙烯颗粒的脱落有关，这些因素被认为是最重要的因素。因此，我们已经决定从这方面进行调查。这是因为它被点出来了，因为服装颗粒的全部臀部颗粒的性质与工业界粉碎的颗粒不同。 ... More 是的，我们制定了一个使用Wear颗粒在整个臀部颗粒中生产的计划，并试着从半胱氨酸组织中提取聚乙烯颗粒。在尝试了各种提取方法之后，这些方法被描述为最实用和最简单的方法。该组织在Xylene中浸泡到溶解PMMA，然后在HNO中浸泡到D23-D2和HCL解决方案。这个解决方案是由缓冲区中性化的。聚乙烯粒子被过滤器聚集在一起。SEM分析中有两种类型的聚乙烯粒子。第一种类型是一个纤维状形状，长度为5.3微米，第二种类型是一个直径为0.2微米。也就是说，我们将对宏观物质进行研究，其中那些已经表征化了这些聚乙烯颗粒，被刺激并产生了PTHrP，样本数量的增加没有被收集到的原因是修订的琼脂糖的低发病率。我们现在正在收集一些样本供调查使用。Less（低）

Our first year study revealed that PTHrP in joint fluid taken during revision total hip arthroplasty was concentrated ten or hundred times higher than that in normal serum and that the pseudmenbranous tissue, which have potential role for bone absorption, was stained positive by anti-PTHrP antibody. Because PTHrP has promoting function for bone absorption, it is strongly suspected that PTHrP relates to the loosening and associated osteolysis of total hip arthroplasty. Based on the results of this study, we have planned the second year experiment to investigate factors that introduce PHTrP. Although many factors are considered to be related to osteolysis associated with the loosening of total hip arthroplasty, polyethylene particle is pointed out to be the most important factor. Therefore, we have decided to investigate from this aspect. Because it was pointed out that the property of the wear particles of total hip articulation is different from that of the industrially crushed particl … More es, we made a plan of experiment using wear particles produced in the total hip articulation, and tried to extract polyethylene particles from psudomenbranous tissue. After trying various methods of extraction, the method described below was revealed to be the most practical and simple. The tissue was soaked in Xylene to solve PMMA and then soaked in HNOィイD23ィエD2 and HCL solution. The solution was neutralized by buffer. Polyethylene particles were gathered by filter. There were two types of polyethylene particles in the tissue by SEM analysis. The first type is a fibril like shape with the length of 5.3 micron in the average and the second type is a spherical shape with the diameter of 0.2 micron in the average. Although we were going to investigate whether macrophages, which had phagocytized these polyethylene particles, were stimulated and produced PTHrP, adequate amount of sample had not been collected because of the low incidence of revision arthroplasty. We are now collecting samples for investigation. Less