男性不妊精子の酸化的DNA障害とその病理学的意義
男性不育精子的氧化DNA损伤及其病理意义
基本信息
- 批准号:09671661
- 负责人:
- 金额:$ 2.05万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In 1997, we investigated whether a high level of oxidative DNA damage in spermatozoa occurs in infertile male patients, and examined the influence of antioxidant treatments on the levels of this damage. The levels of oxidative DNA damage in spermatozoa of infertile male (n= 19) and control patients (n= 17) were compared. In addition, 14 infertile males were given antioxidants for 2 months. The levels of 8-hydroxy-2'-deoxyguanosine, a form of oxidative damage, in the spermatozoa were determined using high performance liquid chromatography with electrochemical detection. The levels of 8-hydroxy-2'-deoxyguanosine in the sperm DNA were significantlyhigher in mate infertile patients than control (1.5*0.2 versus 1.0*0.1/10^5 deoxyguanosine), and were correlated with sperm concentrations in ejaculates. Antioxidant treatment resulted in significant positive effects on sperm concentrations, with a significant reduction in sperm 8-hydroxy-2'-deoxyguanosine levels (from 1.5*0.2 to 1.1*0.14/10^5 d … More eoxyguanosine).In 1998, we investigated the role of radical oxygen species in the testicular germ cell apoptosis induced by heat stress. Testicular cells isolated from immature rats were cultured with or without elevated temperature, and occurrence of apoptosis in these cells was defined by the appearance of DNA fragmentation Following agarose gel electrophoresis, and by the flow cytometric quantification of apoptotic cells. At 32.5゚C, < 1% of cells showed signs of apoptosis throughout the culture period, whereas, under heat stress, the proportion of apoptotic cells increased to 14 % after I h exposure at 43゚C followed by 23 h culture at 32.5 ゚C.Similar to the effect of heat stress, exogenously supplied oxygen free radicals also induced apoptosis. In contrast, treatment with catalase significantly attenuated heat stress-induced apoptosis. Furthermore, heat stress of testicular cells was associated with an increased intracellular peroxide level.Our studies demonstrate an association between the level of oxidative DNA damage in spermatozoa and male infertility, and indicate the involvement of radical oxygen species during testicular germ cell apoptosis induced by heat stress. Less
1997年,我们研究了不育男性患者精子中是否存在高水平的氧化性DNA损伤,并研究了抗氧化治疗对这种损伤水平的影响。比较不育男性(n= 19)和对照组(n= 17)精子中DNA氧化损伤水平。另外,14只不育雄鼠给予抗氧化剂2个月。采用高效液相色谱-电化学检测法测定了精子中8-羟基-2′-脱氧鸟苷(一种氧化损伤)的水平。雄性不育患者精子DNA中8-羟基-2′-脱氧鸟苷水平显著高于对照组(1.5*0.2 vs 1.0*0.1/10^5脱氧鸟苷),且与射精中精子浓度相关。抗氧化处理对精子浓度产生了显著的积极影响,精子8-羟基-2'-脱氧鸟苷水平显著降低(从1.5*0.2降至1.1*0.14/10^5 d…更多的环氧鸟苷)。1998年,我们研究了自由基氧在热应激诱导睾丸生殖细胞凋亡中的作用。对未成熟大鼠睾丸细胞进行高温或不高温培养,通过琼脂糖凝胶电泳后DNA片段的出现和凋亡细胞的流式细胞术定量来确定这些细胞是否发生凋亡。在32.5ºC下,在整个培养过程中,< 1%的细胞表现出凋亡迹象,而在热应激下,在43ºC下暴露1 h,在32.5ºC下培养23 h后,凋亡细胞的比例增加到14%。与热应激作用类似,外源性氧自由基也可诱导细胞凋亡。相反,过氧化氢酶处理能显著减弱热应激诱导的细胞凋亡。此外,睾丸细胞的热应激与细胞内过氧化水平的增加有关。我们的研究证明了精子DNA氧化损伤水平与男性不育之间的关联,并表明自由基氧参与了热应激诱导的睾丸生殖细胞凋亡。少
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kodama H, Ymaguchi R, Fukuda J, Kasai H, Tanaka T.: "Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients." Fertil Steril. 68. 519-524 (1997)
Kodama H、Ymaguchi R、Fukuda J、Kasai H、Tanaka T.:“不育男性患者精子中氧化脱氧核糖核酸损伤增加。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
熊谷 仁,斉藤暁子,池田雅人,福田 淳,兒玉,英也: "酸化ストレスと造精機能" 産科と婦人科. 65. 889-895 (1998)
Hitoshi Kumagai、Akiko Saito、Masato Ikeda、Jun Fukuda、Hideya Kodama:“氧化应激和生精功能”妇产科 65. 889-895 (1998)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kodama H,Yamaguchi R,Fukuda J,Kasai H,Tanaka T.: "Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients." Fertil Steril. 68. 519-524 (1997)
Kodama H、Yamaguchi R、Fukuda J、Kasai H、Tanaka T.:“不育男性患者精子中氧化脱氧核糖核酸损伤增加。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kodama H, Yamaguchi R, Fukuda J, et al.: "Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients." Fertil Steril. 68. 519-524 (1997)
Kodama H、Yamaguchi R、Fukuda J 等人:“不育男性患者精子中氧化脱氧核糖核酸损伤增加。”
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- 发表时间:
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- 影响因子:0
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KODAMA Hideya其他文献
KODAMA Hideya的其他文献
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{{ truncateString('KODAMA Hideya', 18)}}的其他基金
Fundamental studies for development of nursing interventions to improve autonomic neural functioning in the woman in late pregnancy
开发护理干预措施以改善妊娠晚期妇女自主神经功能的基础研究
- 批准号:
23593281 - 财政年份:2011
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
DNA damage of spermatozoa and its influence on embryonal development
精子DNA损伤及其对胚胎发育的影响
- 批准号:
11671592 - 财政年份:1999
- 资助金额:
$ 2.05万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似国自然基金
中国北方人群肺癌患者Cancer/Testis抗原表达谱绘制表位鉴定及功能性抗原特异性CTL制备研究
- 批准号:81673007
- 批准年份:2016
- 资助金额:54.0 万元
- 项目类别:面上项目
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