An Analysis of Sex Steroid Receptors and p53 in Uterine Smooth Muscle Cell Tumors

子宫平滑肌细胞肿瘤中性类固醇受体和 p53 的分析

• 批准号: 09671671
• 负责人: SHIMIZU Motohiko
• 金额: $ 1.92万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671671/
• 关键词: smooth muscle tumors; leiomyosarcoma; Ki-67; cyclin E; cyclin A; cdk2; cdc2; survival analysis

Smooth muscle tumors of the uterus can be divided histologically into benign, malignant, and borderline types. The histological heterogeneity of these smooth muscle tumors suggests that there may be differences in growth potential as well as in growth mechanism among the various types. However, the molecular mechanisms involved in the growth regulation of these tumors are not fully understood. To investigate the cell cycle regulatory machineries involved in the growth mechanism. of smooth muscle tumors, we studied the expression of Ki-67, cyclins E and A, and their catalytic partners, the cyclin-dependent kinases cdk2 and cdc2 using tissue specimens from benign and malignant smooth muscle tumors. These included 20 cases of usual leiomyoma (UL), 18 of cellular leiomyoma (CL), 8 of bizarre leiomyoma (BL), 8 of uncertain malignant potential tumors (UMP.), and 20 of leiomyosarcorna (LMS). The proliferation rate detected by Ki-67 was low in normal myometrium and leiomyomas (UL, CL, and BL), but it was markedly increased in LMS.The expression of the cyclins (E and A), and cdks (cdk2 and cdc2) was also low in normal myometrium and leiomyomas. However, the expression of these factors was markedly increased in LMS.In addition, a survival analysis by Log-rank test revealed that those LMSs with positive staining for cyclin A and with diffusely staining for cyclin E were associated with significantly shorter survival. These results suggest that expression of cyclins and cdks may be involved in the growth control of uterine smooth muscle tumors.

子宫平滑肌肿瘤在组织学上可分为良性、恶性和交界性三种类型。这些平滑肌肿瘤的组织学异质性表明，可能有不同的生长潜力，以及在不同类型之间的生长机制。然而，参与这些肿瘤生长调节的分子机制尚未完全了解。探讨细胞周期调控机制在细胞生长机制中的作用。在平滑肌肿瘤中，我们研究了Ki-67，细胞周期蛋白E和A，以及它们的催化伙伴，细胞周期蛋白依赖性激酶cdk 2和cdc 2的表达，使用组织标本从良性和恶性平滑肌肿瘤。其中普通型平滑肌瘤（UL）20例，富于细胞型平滑肌瘤（CL）18例，奇异型平滑肌瘤（BL）8例，恶性潜能不明型平滑肌瘤（UMP）8例，平滑肌角（LMS）20例。Ki-67在正常子宫肌层和平滑肌瘤（UL、CL和BL）中的增殖率较低，而在LMS中增殖率明显增高，cyclins（E和A）和cdks（cdk 2和cdc 2）在正常子宫肌层和平滑肌瘤中的表达也较低。然而，这些因子的表达显着增加LMS。此外，生存分析的对数秩检验显示，那些LMS与阳性染色的细胞周期蛋白A和弥漫染色的细胞周期蛋白E与显着较短的生存。这些结果表明，细胞周期蛋白和cdks的表达可能参与子宫平滑肌肿瘤的生长控制。

项目成果

A Horiuchi, et al: "Reduced expression of Calponin hl in leiomyosarcoma of the uterus" Laboratory Investigation. 78,7. 839-846 (1998)

A Horiuchi 等人：“子宫平滑肌肉瘤中钙调蛋白 hl 的表达降低”实验室调查。

Ya-Li ZHAI et al: "Expression of cyclins and cyclin-dependent kinase in smooth muscle tumors of the uterus" International J Cancer. in press.

ZHAI ZHAI 等人：“子宫平滑肌肿瘤中细胞周期蛋白和细胞周期蛋白依赖性激酶的表达”国际癌症杂志。

A Horiuchi,et al: "Reduced expression of Calponin h1 in leiomyosarcoma of the uterus" Laboratory Investigation. 78,7. 839-846 (1998)

A Horiuchi 等人：“子宫平滑肌肉瘤中钙调蛋白 h1 的表达减少”实验室调查。

Ya-Li ZHAI et al: "Expression of steroid receptors,Ki-67,and p53 in uterine leiomyosarcomas" International J Gynecol Pathol. 18. 20-28 (1999)

ZHAI ZHAI 等：“子宫平滑肌肉瘤中类固醇受体、Ki-67 和 p53 的表达”国际妇科病理杂志。

Zhai YL,Nikaido T,Shiozawa T,Orii A,Fujii S: "Expression of cyclins and cyclin-dependent kinase in smooth muscle tumors of the uterus" International, J Cancer. (in press).

Zhai YL，Nikaido T，Shiozawa T，Orii A，Fujii S：“子宫平滑肌肿瘤中细胞周期蛋白和细胞周期蛋白依赖性激酶的表达”国际，癌症杂志。