Effect of diabetes mellitus on total amount of bone resorption in denture supporting tissue under continuous pressure

糖尿病对持续压力下义齿支持组织骨吸收总量的影响

• 批准号: 09671987
• 负责人: SATO Takashi
• 金额: $ 0.13万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671987/
• 关键词: Diabetes mellitus; Molar region of palate; Histomorphometry; Amount of bone resorption; Rat; 臼歯部口蓋骨組織; 骨動態

Effect of diabetes mellitus on bone dynamics in denture supporting tissue has not been investigated enough. The purpose of this study was to investigate the effect of diabetes mellitus on bone resorption in the tissues under denture base caused by continuous pressure exerted through denture base by using bone histomorphometry.Two-hundreds and fifty streptozotocin-induced diabetic male rats (n=50 X 5 groups) of Wistar strain were used in this study. Denture base was applied to the molar region of the hard palate at 22 weeks of age. In 3 loaded groups, the denture bases were designed to load a continuous pressure of 1.0, 10.0 or 20.0 kPa to the palate, respectively. In covering groups, the denture base was designed to contact with the palate without any mechanical pressure. The non-denture-wearing group received no intentional treatment. The cleansing of the palate and removable denture base was carried out at 3 or 4 days intervals. Palatal tissue block was excised from 5 rats of each experimental group after the denture insertion at one week interval. The specimens were stained with Villanueva bone stain, embedded in methyl-methacrylate, and ground to 50mum thickness. Eroded surface and osteoclast surface were calculated by using two dimensional graphic analysis system. Amount of bone resorption was calculated as (A) - (B) ; (A) Formed bone tissue during 12 weeks prior to the denture insertion in covering group, (B) Remained bone tissue after the end of bone resorption in each bone resorbed group at each experimental period.Bone resorption was observed in 10.0 and 20.0 kPa groups until 3 and 4 weeks after the denture insertion, respectively. Total amount of bone resorption was 60*16mum in 10.0 kPa group and 87*17mum in 20.0 kPa group. Comparing these results with results in normal rats in same condition as this study, it was showed that diabetes mellitus prolongs the term of bone resorption and increases total amount of bone resorption.

糖尿病对义齿支持组织中骨动力学的影响尚未得到足够的研究。为探讨糖尿病对义齿基托持续压力引起的基托下组织骨吸收的影响，选用Wistar系糖尿病雄性大鼠250只（n=50 × 5组）。22周时，将义齿基托应用于硬腭的磨牙区域。在3个加载组中，义齿基托分别被设计成向腭部施加1.0、10.0和20.0 kPa的持续压力。在覆盖组中，义齿基托被设计成与腭接触而没有任何机械压力。不戴义齿组不接受任何治疗。每隔3或4天进行腭和可摘义齿基托的清洁。实验组和对照组各取5只大鼠，每隔1周，于义齿戴入后切取腭部组织块。标本用Villanueva骨染色剂染色，包埋在甲基丙烯酸甲酯中，并研磨至50 μ m厚。用二维图形分析系统计算侵蚀面和破骨细胞面。骨吸收量按（A）-（B）计算：（A）覆盖组戴入义齿前12周内形成的骨组织，（B）各实验阶段各骨吸收组骨吸收结束后的剩余骨组织，10.0和20.0 kPa组分别观察到戴入义齿后3周和4周的骨吸收。10.0kPa组骨吸收总量为60 × 16 μ m，20.0kPa组为87 × 17 μ m。与正常大鼠在相同条件下的实验结果比较，表明糖尿病延长了骨吸收的时间，增加了骨吸收的总量。